A5008400928- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Chromatin Remodeling and Cancer
- Eicosanoids and Hypertension Pharmacology
- Cancer Research and Treatments
- Diet and metabolism studies
- Molecular Biology Techniques and Applications
- Cancer Mechanisms and Therapy
- Protein Degradation and Inhibitors
- Virus-based gene therapy research
- Cell death mechanisms and regulation
- Transgenic Plants and Applications
- Metabolism and Genetic Disorders
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Viral gastroenteritis research and epidemiology
- Peptidase Inhibition and Analysis
- Antimicrobial Peptides and Activities
- Autoimmune Bullous Skin Diseases
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- Viral Infections and Immunology Research
- Genomics and Chromatin Dynamics
- Drug-Induced Adverse Reactions
Salk Institute for Biological Studies
2006-2008
Scripps Research Institute
2008
Swiss Group For Clinical Cancer Research
2001-2004
p53 can adopt two forms in vitro , a latent form that binds naked DNA poorly and an active well. Conversion of the to is thought occur by allosteric mechanism induced phosphorylation acetylation. Despite large differences affinity produced regulatory modifications mutation putative sites has not correspondingly effects on transcription target genes vivo . To determine whether genotoxic stress regulates binding we have performed quantitative chromatin immunoprecipitation (ChIP) assays tumor...
The composition of chromatin-remodeling complexes dictates how these enzymes control transcriptional programs and cellular identity. In the present study we investigated SWI/SNF in embryonic stem cells (ESCs). contrast to differentiated cells, ESCs have a biased incorporation certain paralogous subunits with low levels BRM, BAF170, ARID1B. Upon differentiation, expression increases, resulting higher diversity compositionally distinct enzymes. We also identified BRD7 as novel component...
ABSTRACT Vaginal inflammation (vaginitis) is the most common disease caused by human-pathogenic fungus Candida albicans . Secretory aspartyl proteinases (Sap) are major virulence traits of C. that have been suggested to play a role in vaginitis. To dissect mechanisms which Sap this role, Sap2, dominantly expressed member family and putative constituent an anti- vaccine, was used. Injection full-length Sap2 into mouse vagina local neutrophil influx accumulation inflammasome-dependent...
Phosphorylation of mouse p53 at Ser18 occurs after DNA damage. To determine the physiological roles this phosphorylation event in p53-dependent damage responses, a to Ala missense mutation was introduced into germline mice. Thymocytes and fibroblasts from knock-in mice show reduced transactivation many target genes following protein stabilization binding are similar wild type mice, but C-terminal acetylation defective, consistent with role for recruitment transcriptional co-activators. The...
We have used a lentiviral vector to stably express p53 at physiological level in knockout HCT116 cells. Cells transduced with wild type responded genotoxic stress by stabilizing and expressing target genes. The reconstituted cells underwent G1 arrest or apoptosis appropriately depending on the of stress, albeit less efficiently than parental Compared exogenous p53, apoptotic response 5-fluorouracil (5FU) was >50% reduced S15A S20A mutant even more combined mutation serines 6, 9, 15, 20, 33,...
Conference Abstract| June 01 2001 Mitochondrial AAA proteases: Control of biogenesis and maintenance respiratory chain complexes K. Leonhard; Leonhard 1Institut fuer Physiologische Chemie, Universitaet Muenchen, Goethestr. 33, 80336 Muenchen Search for other works by this author on: This Site PubMed Google Scholar M. Kaeser; Kaeser Kambacheld; Kambacheld C. Klanner; Klanner L. Young; Young T. Langer Biochem Soc Trans (2001) 29 (3): A52. https://doi.org/10.1042/bst029a052a Views Icon Article...