A5008124146- Pancreatic function and diabetes
- Diabetes Management and Research
- Diabetes and associated disorders
- Diabetes Treatment and Management
- Zebrafish Biomedical Research Applications
- Angiogenesis and VEGF in Cancer
- Tendon Structure and Treatment
- Congenital heart defects research
- Mesenchymal stem cell research
- Orthopedic Surgery and Rehabilitation
- Cannabis and Cannabinoid Research
- Electrolyte and hormonal disorders
- RNA modifications and cancer
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Cancer Mechanisms and Therapy
- Genetics and Neurodevelopmental Disorders
- Diet and metabolism studies
- Nutrition and Health in Aging
- Diet, Metabolism, and Disease
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Adenosine and Purinergic Signaling
- Pluripotent Stem Cells Research
- Metabolism, Diabetes, and Cancer
- Shoulder Injury and Treatment
Vanderbilt University
2014-2025
Vanderbilt University Medical Center
2014-2024
Boston Children's Hospital
2009-2023
Joslin Diabetes Center
2023
Harvard University
2021
Boston Children's Museum
2011
Metastatic tumors can prepare a distant site for colonization via the secretion of factors that act in systemic manner. We hypothesized non- or weakly metastatic human tumor cells may an opposite fashion by creating microenvironment tissues is refractory to colonization. By comparing cell lines with different potential, we have identified tumor-secreted inhibitor metastasis, prosaposin (Psap), which functions paracrine and endocrine stimulating expression thrombospondin-1 (Tsp-1) fibroblasts...
Neurovascular alignment is a common anatomical feature of organs, but the mechanisms leading to this arrangement are incompletely understood. Here, we show that vascular endothelial growth factor (VEGF) signaling profoundly affects both vascularization and innervation pancreatic islet. In mature islets, nerves closely associated with capillaries, islet process during embryonic organogenesis significantly precedes innervation. Although simple neuronal meshwork interconnects developing...
Umbilical cord blood (UCB) is recognized as an enriched source of endothelial progenitor cells (EPCs) with potential therapeutic value. Because cryopreservation the only reliable method for long-term storage UCB cells, clinical application EPCs depends on our ability to acquire them from cryopreserved samples; however, feasibility doing so remains unclear. In this study we demonstrate that can be isolated UCB-derived mononuclear (MNCs). The number outgrowth EPC colonies emerged in culture...
Numerous compounds stimulate rodent β-cell proliferation; however, translating these findings to human β-cells remains a challenge. To examine proliferation in response such compounds, we developed medium-throughput vitro method of quantifying adult markers. This is based on high-content imaging dispersed islet cells seeded 384-well plates and automated cell counting that identifies fluorescently labeled with high specificity using both nuclear cytoplasmic β-Cells from each donor were...
Endogenous β cell regeneration could alleviate diabetes, but proliferative stimuli within the islet microenvironment are incompletely understood. We previously found that recovery following hypervascularization-induced loss involves interactions with endothelial cells (ECs) and macrophages (MΦs). Here we show ECs modulate MΦ infiltration phenotype during loss, recruited MΦs essential for recovery. Furthermore, VEGFR2 inactivation in quiescent accelerates vascular regression leads to...
ABSTRACT Human endocrine cell differentiation and islet morphogenesis play critical roles in determining mass function, but the events timeline of these processes are incompletely defined. To better understand early human development maturation, we collected 115 pediatric pancreata mapped morphological spatiotemporal changes from birth through first ten years life. Using quantitative analyses a combination complementary tissue imaging approaches, including confocal microscopy whole-slide...
Obesity is a worsening global health crisis leading to increased risk of long-term complications such as type 2 diabetes (T2D), nonalcoholic steatohepatitis (NASH), dyslipidemia and cardiovascular disease. Identifying novel molecular mechanisms regulating the connection between obesity, hepatic insulin resistance progression NASH critical develop new therapeutic options for these patients. Previous work has shown that reversible N6-methyladenosine (m6A) RNA methylation patterns are altered...
Background: Education and management for new onset diabetes (NOD) does not always require hospitalization. A pathway outpatient of pediatric NOD patients after initial evaluation long-acting insulin dose in the emergency department was developed at a tertiary academic medical center. Methods: multidisciplinary team identified key eligibility criteria including absence DKA, age ≥3 years, beta-hydroxybutyrate (BOHB) <1 mmol/L. Data tracking, chart review, endocrine provider surveys...
ABSTRACT Endogenous β cell regeneration could alleviate diabetes, but proliferative stimuli within the islet microenvironment are incompletely understood. We previously found that recovery following hypervascularization-induced loss involves interactions with endothelial cells (ECs) and macrophages (MΦs). Here we show ECs modulate MΦ infiltration phenotype during loss, recruited MΦs essential for recovery. Furthermore, VEGFR2 inactivation in quiescent accelerates vascular regression leads to...