A5091243070- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Methane Hydrates and Related Phenomena
- Marine Biology and Ecology Research
- Genomics and Phylogenetic Studies
- Lipid Membrane Structure and Behavior
- Wastewater Treatment and Nitrogen Removal
- Protist diversity and phylogeny
- Geology and Paleoclimatology Research
- Coastal wetland ecosystem dynamics
- Infant Nutrition and Health
- Innovative Teaching Methods
- Paleontology and Stratigraphy of Fossils
- Ocean Acidification Effects and Responses
- Environmental DNA in Biodiversity Studies
- Experimental Learning in Engineering
- Drug Transport and Resistance Mechanisms
- Arctic and Antarctic ice dynamics
- Field-Flow Fractionation Techniques
- Cephalopods and Marine Biology
- Freezing and Crystallization Processes
- Nanoparticle-Based Drug Delivery
- Glycosylation and Glycoproteins Research
- Phase Equilibria and Thermodynamics
- Groundwater and Isotope Geochemistry
University of Southern Denmark
2013-2023
Oregon State University
2007-2014
Czech Academy of Sciences, Institute of Microbiology
2011
University of Bergen
2005
Technical University of Darmstadt
2002-2005
Summary Mesophilic crenarchaeota are frequently found in terrestrial and marine habitats worldwide, but despite their considerable abundance the physiology of these as yet uncultivated archaea has remained unknown. From a 1.2 Gb large‐insert environmental fosmid library calcareous grassland soil, 43 kb genomic fragment was isolated with ribosomal RNA that shows its affiliation to group 1.1b repeatedly soils. The insert encoded homologue copper‐containing nitrite reductase an unusual...
Significance The relationship between the origin of animals and oxygen content atmosphere is a pressing issue in historical geology. Here we challenge widely held view that low levels atmospheric delayed up until 850–542 million years ago. We provide experimental evidence suggesting last common ancestor could have thrived as 0.5% to 4% present levels, which were likely met on Earth well before evolved. This was achieved by observing survival sponges, basal similar earliest metazoans, under...
Summary Acidobacteria have been established as a novel phylum of Bacteria that is consistently detected in many different habitats around the globe by 16S rDNA‐based molecular surveys. The phylogenetic diversity, ubiquity and abundance this group, particularly soil habitats, suggest an important ecological role extensive metabolic versatility. However, genetic physiological information about scarce. In order to gain insight into genome structure, evolution diversity these microorganisms we...
Potential benefits of simulations and virtual laboratory exercises in natural sciences have been both theorised studied recently. This study reports findings from a pilot on student attitude, motivation self-efficacy when using the programme Labster. The allows interactive learning about workflows procedures biological biochemical experiments, operation relevant apparatuses, including ability to adjust parameters, production results. was used as supplement mandatory two undergraduate courses...
Summary Molecular phylogenetic surveys based on the characterization of 16S rRNA genes have revealed that soil is an environment particularly rich in microbial diversity. A clade crenarchaeota (archaea) has frequently been detected among many other novel lineages uncultivated bacteria. In this study we initiated a genomic approach for microorganisms from soil. We developed procedure two‐phase electrophoresis technique allows fast and reliable purification concentrated clonable, high...
Summary Complex genomic libraries are increasingly being used to retrieve complete genes, operons or large fragments directly from environmental samples, without the need cultivate respective microorganisms. We report on construction of three large‐insert fosmid in total covering 3 Gbp community DNA two different soil a sandy ecosystem and mixed forest soil. In end sequencing approach including 5376 sequence tags ≈ 700 bp length, we show that mostly bacterial and, much lesser extent,...
In a 2.5-year-long environmental engineering experiment in the By Fjord, surface water was pumped into deepwater where frequency of renewals increased by factor 10. During experiment, became long-term oxic, and nitrate dominating dissolved inorganic nitrogen component. The amount phosphate column decreased 5 due to increase flushing reduction leakage from sediments when sediment oxidized. Oxygenation did not toxic metals organic pollutants. bacterial community first show changes after...
The functionality of the plasma membrane is essential for all organisms. Adaption to high growth temperatures imposes challenges and Bacteria, Eukarya, Archaea have developed several mechanisms cope with these. Hyperthermophilic archaea earlier been shown synthesize tetraether lipids an increased number cyclopentane moieties at higher temperatures. Here we used shotgun lipidomics study this effect as well influence phase on lipidomes Sulfolobus islandicus tokodaii first time. Both species...
The molecular structures, biosynthetic pathways and physiological functions of membrane lipids produced by organisms in the domain Archaea are poorly characterized as compared with that counterparts Bacteria Eukaryota. Here we report on use high-resolution shotgun lipidomics to characterize, for first time, lipid complement archaeon Sulfolobus islandicus. To support identification S. islandicus, compiled a database ether species previously ascribed Archaea. Next, analyzed islandicus Fourier...
Anaerobic ammonium oxidizing (anammox) bacteria own a central position in the global N-cycle, as they have ability to oxidize NH4+ N2 under anoxic conditions using NO2-. They are responsible for up 50% of all released from marine ecosystems into atmosphere and thus indispensible balancing activity N-fixing completing N-cycle. The contribution, diversity, impact anammox freshwater ecosystems, however, is largely unknown, confounding assessments their role Here we report diversity world's...
For oral drug delivery the stability of liposomes against intestinal bile salts is key importance. Here, asymmetric flow field-flow fractionation (AF4) coupled to multi-angle laser light scattering (MALLS) and a differential refractive index (dRI) detector was employed monitor structural re-arrangement upon exposure model salt taurocholate. comparison, conventional assay using hydrophilic marker size exclusion chromatography (SEC) separate released from liposome-entrapped dye....
Evidence for recent volcanic eruptions along the fast spreading East Pacific Rise (EPR) crest near 9°50′N spanning about 4 to 5 months of activity was discovered in April and May 2006 as a result studies related U.S. National Science Foundation' (NSF) Ridge2000 (R2K) program. In April, during routine recovery redeployment ocean‐bottom seismometers (OBS) at EPR R2K Integrated Study Site (ISS) 9°50′N, eight 12 OBS could not be recovered [ Tolstoy et al, 2006]. Anomalous turbidity temperature...
Understanding bacterioplankton community dynamics in coastal hypoxic environments is relevant to global biogeochemistry because hypoxia increasing worldwide. The temporal of communities were analysed throughout the illuminated water column Devil's Hole, Bermuda during 6-week annual transition from a strongly stratified with suboxic and high-pCO2 bottom waters fully mixed ventilated state 2008. A suite culture-independent methods provided quantitative spatiotemporal characterization changes,...
Coastal areas have become more prone to flooding with seawater due climate-change-induced sea-level rise and intensified storm surges. One way cope this issue is by "managed coastal realignment", where low-lying are no longer protected instead flooded seawater. How impacts soil microbiomes the biogeochemical cycling of elements poorly understood. To address this, we conducted a microcosm experiment using cores collected at nature restoration project site Gyldensteen Strand (Denmark), which...
Abstract. Permanent flooding of low-lying coastal areas is a growing threat due to climate change and related sea-level rise. An increasingly common solution protect lying below sea level intentional by "managed realignment". However, the biogeochemical implications agricultural soils with seawater are still not well understood. We conducted 1-year mesocosm experiment investigate microbial carbon degradation processes in flooded seawater. Agricultural were sampled on northern coast island...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 79:85-99 (2017) - DOI: https://doi.org/10.3354/ame01821 Nitrogen cycling and bacterial community structure of sinking aging diatom aggregates Ann Sofie Birch Lundgaard1,*, Alexander H. Treusch1, Peter Stief1, Bo Thamdrup1, Ronnie N. Glud1,2,3 1Department Biology Nordic Center for Earth Evolution, University Southern...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 64:15-25 (2011) - DOI: https://doi.org/10.3354/ame01504 Bacterial diversity in bottom boundary layer of inner continental shelf Oregon, USA A. D. Bertagnolli1, H. Treusch1, O. U. Mason2, Stingl4, K. L. Vergin1, F. Chan3, B. Beszteri1, S. J. Giovannoni1,* 1Department Microbiology, 2College Oceanic and Atmospheric...