ABSTRACT Interspecific polymorphisms of the 16S rRNA gene (rDNA) are widely used for species identification mycobacteria. rDNA sequences, however, do not vary greatly within a species, and they either indistinguishable in some example, Mycobacterium kansasii M. gastri , or highly similar, malmoense szulgai . We determined 16S-23S internal transcribed spacer (ITS) sequences 60 strains genus representing 13 ( avium conspicuum genavense marinum shimoidei simiae triplex ulcerans xenopi ). An...

Abstract Background Human brucellosis is an infectious zoonotic disease caused by Brucella spp. It one of the most public health problems that remains largely neglected in developing counties, including Saudi Arabia. Brucellosis particularly prevalent among rural people who have constant contact with livestock. Methods A cross-sectional sero-epidemiological study conducted Aseer Central Hospital, South Arabia, between 2014 and 2018 7567 patients. Serum samples were analyzed for antibodies...

Microbial infections of the vagina in pregnant women are health problems that lead to serious medical complications and consequences. This study aimed investigate determine antimicrobial susceptibilities causative agents vaginal women.A cross-sectional (n = 200) was conducted between August December 2008 at Omdurman Maternity Hospital, Khartoum, Sudan. Vaginal cervical swabs were obtained from each subject processed for isolation identification pathogenic microorganisms using standard...

To identify and to determine the antimicrobial susceptibility of Acinetobacter baumannii (A. baumannii) clinical isolates from ICU at Aseer Central Hospital. The study was conducted in Intensive Care Unit, Hospital, Saudi Arabia over 13 months period (2014–2015). species (n = 105) were isolated various samples. Isolates identified using selected phenotypic criteria confirmed Vitek 2 automated system. This system used susceptibilities 21 agents. Patients, drug data analyzed SPSS statistical...

Nosocomial infections caused by Klebsiella pneumoniae and other Gram-negative organisms have emerged as a significant health problem especially in intensive care units (ICU). This study aims to examine K. the ICU of Aseer Central Hospital determine their antimicrobial susceptibility relationship patients' clinical outcomes. is retrospective observational done tertiary center region Saudi Arabia. The spanned from January 2018 December 2019. Demographic, microbiologic, patient outcomes were...

the emergence of antibiotic resistance pathogens is an important health risk. Usually Gram negative bacteria acquire to beta-lactam antibiotics by beta-lactamase production. The objectives this study was assess prevalence ESBL and detect frequency blaTEM, blaSHV blaCTX-M genotypes among producing Enterobacteriaceae isolates from patients in Khartoum, Sudan.a total 171 were recovered hospitals Sudan (2014 -2015) used production using disc diffusion method. genes investigated PCR based methods...

This study aims to analyze the importance of professional laws and codes ethics for members National Police Republic Indonesia (Polri). The code is a conduct that must be followed by every member in carrying out their duties responsibilities. examines legal aspects govern ethics, as well evaluating challenges its implementation police practice. Data was collected through literature studies, analysis regulations, in-depth interviews with Police. results show effective enforcement can...

Post-kala-azar dermal leishmaniasis (PKDL) is a neglected tropical disease that can develop after treatment of leishmaniasis. It causes significant health risks and serves as reservoir, perpetuating transmission. Current information on PKDL characteristics crucial for effective management control. This study aimed to describe clinical epidemiological patients in eastern Sudan. A retrospective cross-sectional was conducted suspected ( N = 37) at tertiary hospital Blood samples were tested...

Rapid dissemination of Klebsiella pneumoniae carbapenemase (KPC) is a leading cause treatment failure, significantly increasing morbidity and mortality rates among inpatients, particularly in the intensive care unit (ICU). This study aimed to detect occurrence carbapenemase- carbapenem-resistant-encoding genes K. isolates from COVID-19 positive negative patients, assess their impact on patient outcomes. A prospective analysis was conducted at tertiary hospital Saudi Arabia, collecting 97...

Background: Visceral leishmaniasis (VL) is a neglected tropical disease common in Sudan. Treatment outcomes are affected by factors such as drug resistance. Despite the accessibility of antileishmanial drugs, vary regionally. Identification epidemiology, clinicopathology, and contributing important for informed treatment decision-making. Aim: This retrospective study aimed to investigate clinical manifestations VL evaluate first- secondline outcomes. Methods: The included 363 patients with...

Eight actinomycete strains, isolated from 8 out of 400 sputum samples examined, taken patients with pulmonary diseases at the Chest Unit Khartoum Teaching Hospital in Sudan, were provisionally assigned to genus Nocardia according morphological criteria. These isolates studied further order establish their taxonomic status. They found have and chemical properties typical nocardiae formed a monophyletic clade 16S ribosomal DNA tree together vaccinii. The strains showed unique pattern...

The study aims to determine the prevalence of multidrug-resistant A. baumannii in Aseer Region, Kingdom Saudi Arabia.This evaluated antibiotic susceptibility ninety-four (n = 94) clinical isolates baumannii. were collected from south region Arabia, and notably during period 15 October 2014 January 2015. tentatively identified as by routine bench tests confirmed using VITEK® 2 Compact. latest instrument was used identify these isolates.Antibiotic this showed that 69% strains. Moreover, they...

This study aimed to determine the prevalence and assess antimicrobial susceptibility of extended- spectrum β-lactamase-producing Escherichia coli isolated from clinical specimens patients at hospitals in Khartoum State, Sudan.During April August 2011, a total 232 E. isolates were collected various patients. Isolates identified, tested for screened ESBL production as per standard methods. The double-disk diffusion method was used confirm using disks ceftazidime (30 μg), cefotaxime with or...

Summary One‐hundred‐and‐twenty caseous lesions collected from slaughtered cattle at selected slaughterhouses in Sudan were processed for the detection of acid‐fast bacteria (AFB). Sixty‐four 120 samples showed AFB on microscopic examination after staining with Ziehl–Neelsen method. Accordingly, it was estimated that 64 (53.3%) (purulent) among due to whereas 56 (46.7%) other causes. Growth Lowenstein‐Jensen slants obtained 54 samples. The isolated tentatively identified using and cultural...

16S ribosomal DNA (rDNA) and 16S-23S internal transcribed spacer rDNA sequence analyses were performed on Mycobacterium farcinogenes M. senegalense strains 26 of other rapidly growing mycobacteria to investigate the phylogenetic structure bovine farcy within fortuitum complex. indistinguishable in their 5"-end but showed both considerable interspecies divergence a high level intraspecies stability. A rapid detection assay using PCR hybridization with species-specific probes was developed....

SUMMARY: Two hundred and fifty-two representatives of the genera Corynebacterium, Gordona, Mycobacterium, Nocardia, Rhodococcus Tsukamurella were degraded by alkaline hydrolysis their mycolic acids extracted as methyl esters following phase-transfer-catalysed esterification. When acid treated with a mixture acetonitrile toluene all mycobacterial mycolates formed copious white precipitates whereas but 5 out 106 non-mycobacterial remained in solution. The precipitated dried soluble compared...

Mangifera indica L. is a species of mango in the Anacardiaceae family. It found wild tropical regions and cultivated varieties have been introduced to other warm world. This present study aimed investigate vitro antimicrobial activities methanol ethanol extracts seed against 25 representatives gram positive, negative, acid fast bacteria fungi. Mango fruit were extracted by Soxhlet using as solvents. The tested microorganisms disc diffusion method at different concentrations: 5 mg/mL, 3.75...

Highlights•The diagnosis of tuberculosis (TB) in children is challenging due to insufficient specimen material and the scarcity bacilli specimens.•This study aimed evaluate diagnostic methods for diagnosing childhood TB Sudan.•The situation regarding pediatric Sudan its prevalence are discussed, since known as one developing countries that suffers from emergence such fatal disease lack policies regulations control outbreaks community-based infections.SummaryObjectivesThe specimens. This...

Background Beta-lactamase enzymes-producing Enterobacteriaceae have emerged in many hospital settings resulting poor treatment outcomes. We aimed to determine resistant patterns of enzymes among collected from referral hospitals Khartoum state, Sudan. Methods A total 168 recovered clinical samples patients during May 2014 February 2015. Identification and susceptibility testing the isolates were performed as per standard methods. Double-disk synergy test was applied presence...