The aims of this study were to test the essential oil from Lippia sidoides Cham. for antifungal activity, in vitro, against Candida spp. and Microsporum canis, evaluate its acute subchronic toxicological effects, vivo, determine chemical constituents. was initially evaluated by agar-well diffusion technique, MIC minimum fungicidal concentration (MFC) determined broth microdilution method. effects mice rats, respectively. composition gas chromatography coupled mass spectroscopy. obtained L....

The present study had the aim of testing hexane and methanol extracts avocado seeds, in order to determine their toxicity towards Artemia salina, evaluate larvicidal activity Aedes aegypti investigate vitro antifungal potential against strains Candida spp, Cryptococcus neoformans Malassezia pachydermatis through microdilution technique. In tests on from seeds showed LC50 values 2.37 24.13 mg mL-1 respectively. Against larvae, results obtained were 16.7 for extract 8.87 seeds. tested also...

This study aimed to determine the minimum inhibitory concentration (MIC) of kaempferol and quercetin against planktonic biofilm forms Candida parapsilosis complex. Initially, nine C. sensu stricto, orthopsilosis metapsilosis strains were used. Planktonic susceptibility was assessed. Growing mature biofilms then exposed flavonoids at MIC or 10xMIC, respectively, theywere also analyzed by confocal laser scanning microscopy. The ranges 32-128 µg ml−1 for 0.5-16 quercetin. Kaempferol decreased...

To find new antifungal agents among essential oils from Brazilian Croton species.Plant leaves were steam distilled and the obtained analyzed by gas chromatography/mass spectroscopy. The main constituents estragole anethole for zehntneri, methyl-eugenol bicyclogermacrene nepetaefolius spathulenol argyrophylloides. activity of was evaluated against Candida albicans, tropicalis Microsporum canis agar-well diffusion method minimum inhibitory concentration (MIC) broth microdilution method....

The objective of this study was to evaluate the antifungal activity farnesol and its interaction with traditional antifungals against drug-resistant strains Candida species. To do so, we studied minimum in vitro inhibitory concentration (MIC) amphotericin B (AMB), fluconazole (FLC), itraconazole (ITC), caspofungin (CAS) 45 isolates spp., i.e., 24 C. albicans, 16 parapsilosis 5 tropicalis through use broth microdilution method. Then, were tested combination plus drugs which they previously...

Difficulties in the treatment of Candida spp. invasive infections are usually related to formation biofilms. The aim this study was determine effects molecular weight (MW) chitosan (using high (HMW), medium (MMW) and low (LMW) chitosan) on albicans, tropicalis parapsilosis sensu stricto. deacetylation degree (DD) M were measured by potentiometric titration viscosimetry, respectively. planktonic shape activity quantified broth microdilution, against biofilm metabolic through XTT...

Histoplasmosis is a serious infectious disease in humans caused by Histoplasma spp. (Onygenales), whose natural reservoirs are thought to be soil enriched with bird and bat guano. The true global burden of histoplasmosis underestimated frequently the pulmonary manifestations misdiagnosed as tuberculosis. Molecular data on epidemiology still scarce, even though there increasing recognition recent years areas distant from traditional endemic regions Americas. We used multi-locus sequence...

The aim of the study was to produce and characterize chitosan microparticles loaded with essential oils (CMEOs), evaluate oil (EO) release profile CMEOs' anti-Candida activity. (CMs) lemongrass (LEO) geranium (GEO) were produced by spray-drying method characterized regarding CMEO morphological physicochemical parameters EO encapsulation efficiency (EE) profile. planktonic activity quantified broth microdilution, against biofilm biomass formation measurement. LEO GEO compositions analyzed gas...

The ocular microflora in dogs has not been established north-east Brazil. Thus, the main aim of this research was to determine bacterial microorganisms conjunctival sac clinically normal and with ulcerative keratitis Fortaleza, Ceará, Brazil.This study included 60 healthy dogs, 15 unilateral corneal ulcer, three bilateral ulcers. Procedure Samples were taken by a calibrated platinum loop (1 microL) placed directly onto on sterile blood agar. clinical specimens incubated at 37 degrees C an...

The aim of this study was to perform a retrospective analysis cases candidemia in Brazilian hospital the city Fortaleza, Ceará. A total 50 blood cultures were analyzed from 40 candidemic patients. mycological diagnosis based on phenotypical and patients' data recorded appropriate files. most frequent species Candida parapsilosis (n = 18), followed by C. albicans 14), tropicalis 8), guillermondii 6), glabrata 2), spp. 2). detailed descriptive undertaken with 21 patients whose medical records...

Cockatiels are the world's second most popular psittacine pet bird, but no data characterizing their gastrointestinal microbiota have been found. Thus, aim of this work was to characterize yeast cockatiels and evaluate relevance as carriers potentially pathogenic yeasts. A total 60 cockatiels, from 15 different premises, were assessed. thorough clinical examination performed with each samples collected oral cavity, crop cloaca. The stools cages where birds kept. isolates identified according...

<h3>Objective:</h3> This study aimed to determine the frequency of CNS infection by dengue virus (DENV) in individuals with fatal outcomes. <h3>Methods:</h3> Samples 150 suspect an disease and outcomes were investigated for evidence presence DENV. The sampling was made up CSF, 120 tissue samples, 109 blood specimens. tests used viral isolation, reverse transcriptase PCR, immunohistochemistry, nonstructural 1 antigen, immunoglobulin M detection. <h3>Results:</h3> Out studied patients, 84...

Candida tropicalis has been associated with invasive candidiasis, being the first or second most common non-Candida albicans species isolated in humans candidemia and candiduria, as well frequently from healthy animals. This study aimed to characterize C. isolates (n = 64) obtained several animal regarding antifungal susceptibility production of virulence factors. The were microbiota animals (goats, n 25; sheep, 6; psittacines, 14; rheas, horses, 2; sirenians, 5; shrimp, 1), aquatic mammals...

This study contains a descriptive analysis of histoplasmosis in AIDS patients between 2006 and 2010 the state Ceará, Brazil. Additionally, vitro susceptibility Histoplasma capsulatum isolates obtained during this period was assessed. We report 208 cases with AIDS, describing epidemiological, clinical, laboratory therapeutic aspects. The antifungal test carried out by microdilution method, according to Clinical Laboratory Standards Institute, H. filamentous yeast phases, against antifungals...

ABSTRACT There have recently been significant increases in the prevalence of systemic invasive fungal infections. However, number antifungal drugs on market is limited comparison to available antibacterial drugs. This fact, coupled with increased frequency cross-resistance, makes it necessary develop new therapeutic strategies. Combination drug therapies become one most widely used and effective strategies alleviate this problem. Amiodarone (AMD) classically for treatment atrial fibrillation...

Heat-shock proteins (Hsps) are chaperones required for the maintenance of cellular homeostasis in different fungal pathogens, playing an important role infectious process. This study investigated effect pharmacological inhibition Hsp90 by radicicol on Cryptococcus neoformans/Cryptococcus gattii species complex--agents most common life-threatening infection amongst immunocompromised patients. The influence was regarding vitro susceptibility to antifungal agents planktonic and sessile cells,...

Tyrosol is a quorum-sensing molecule of Candida albicans able to induce hyphal development in the early and intermediate stages biofilm growth. In present study, we evaluated effect high concentrations exogenous tyrosol on planktonic cells biofilms C. (n = 10) tropicalis 10), investigated whether could be synergic antifungals that target cellular ergosterol. Antifungal susceptibility drug interaction against were by broth microdilution method. was inhibit cells, with MIC values ranging from...

The aim of this study was to evaluate the in vitro and ex vivo biofilm-forming ability dermatophytes on a nail fragment.Initially, four isolates Trichophyton rubrum, six tonsurans, three mentagrophytes, ten Microsporum canis gypseum were tested for production biomass by crystal violet assay. Then, one strain per species presenting best biofilm chosen further studies optical microscopy (Congo red staining), confocal laser scanning (LIVE/DEAD staining) electron (secondary electron)...

Rabies is an endemic disease in Brazil, where it considered a serious public health problem. Although the number of human and dog-transmitted cases has declined recent decades, rabies wildlife emerged considerably. Among sylvatic animals, wild canids have been important hosts virus. We performed retrospective study reported victims Ceará state (Northeast Brazil) during 2003 to 2013. Information was provided by governmental laboratories involved detection Ministry Health. From January...

This study aimed to evaluate the in vitro antifungal activity of terpinen-4-ol, tyrosol, and β-lapachone against strains Coccidioides posadasii filamentous phase (n=22) Histoplasma capsulatum both (n=40) yeast phases (n=13), using broth dilution methods as described by Clinical Laboratory Standards Institute, determine minimum inhibitory concentration (MIC) fungicidal (MFC) these compounds. The mechanisms action compounds were also investigated analyzing their effect on cell membrane...