A5075362296- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Chromatography in Natural Products
- Protein purification and stability
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Chemical Sensor Technologies
- Analytical Methods in Pharmaceuticals
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Sensors
- Synthesis and Reactions of Organic Compounds
- Sulfur-Based Synthesis Techniques
- Synthesis of heterocyclic compounds
- Metabolomics and Mass Spectrometry Studies
- Synthesis and Catalytic Reactions
- Computational Drug Discovery Methods
- Electrochemical Analysis and Applications
- Estrogen and related hormone effects
- Organic Chemistry Cycloaddition Reactions
- Synthesis and Biological Activity
- Phenothiazines and Benzothiazines Synthesis and Activities
- Synthesis and Biological Evaluation
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Crystallization and Solubility Studies
- Structural and Chemical Analysis of Organic and Inorganic Compounds
- Advanced Synthetic Organic Chemistry
University of Strathclyde
2012-2025
Shimadzu (United Kingdom)
2019-2025
The Open University
2022-2024
Shimadzu (China)
2022-2023
Loughborough University
2001-2012
AstraZeneca (United Kingdom)
2000-2012
AstraZeneca (Brazil)
2004-2008
Leicester Royal Infirmary
2008
University of Manchester
1981-1991
University of London
1985-1990
Capillary electrochromatography (CEC) has seen a resurgence of interest during the 1990s, despite having origins in 1970s. The technique combines desirable features both high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE): separation process is based on differential interactions between stationary mobile phases, whilst electroosmotic flow transports phase through capillary. Thus, it demonstrated advantages over HPLC CE, which are yet to be fully exploited wide...
The operational parameters and factors which control the performance of capillary electrochromatography (CEC) using commercially available CEC instrumentation are evaluated discussed. neutral or ion-suppressed acidic analytes shows marked advantages such as increased column efficiency reduced analysis times compared to conventional high-performance liquid chromatography (HPLC). Development method C-18 stationary phases follows similar guidelines that HPLC and, due high efficiencies obtained,...
Abstract A range of ten perfluorophenyl and perfluoroalkyl stationary phases has been evaluated using standard chromatographic tests probes. Principal Component Analysis the data indicated that can be divided into distinct groupings. Extending dataset to include alkyl phenyl provided further interpretation support orthogonal selectivity claims made for perfluorinated phases. The analysis a basic analytes showed an unusual extended retention hydrophilic with perfluorophases. Furthermore,...
The separation of ionized bases by reversed-phase liquid chromatography with alkyl silica columns often leads to severely tailed bands that are highly detrimental. Band shape and its dependence on sample mass notably different when mobile-phase pH is changed, this behavior has not been previously explained. Ionized silanols present in the stationary phase have credited a role determining peak shape. In study, separations two polymer were compared those obtained phases. Because absent from...
Abstract The practical effects of gradient time and flow rate on the peak capacities a range analytes differing molecular weights (MWs) physico‐chemical properties have been evaluated using ultra high pressure LC instrumentation with sub‐2 μm superficially porous particle phases. Optimum capacity, in RP LC, for small molecules, including typical pharmaceutical drugs peptides MWs up to 1300, was demonstrated at maximum given ( i.e . 40 min). Flow rates significantly higher than optimum van...
Capillary electrochromatography (CEC) has proved to be applicable the separation of neutral and acidic (chromatographed in their ion-suppressed mode) compounds but a major factor holding back its further development been perceived inability analyse basic compounds. In this paper we demonstrate that addition competing base, such as triethylamine (TEA) or triethanolamine (TEOA), low pH buffer can achieve excellent CEC analysis range strong pharmaceutical bases. Acceptable peak symmetry...
Rapid and highly efficient separations of pharmaceutical compounds can be achieved by capillary electrochromatography (CEC) in analyses which are quantitative, reproducible robust. Columns prepared packing with a supercritical fluid were gave repeatable, quantitative sensitive separations. Pressurization the system control column temperature necessary to prevent bubble formation. The concept isoeluotropic strength was shown apply CEC. Applications described.
Abstract Eight commercially available sub‐2 μm octadecyl silane columns (C18 columns) have been characterised by the Tanaka protocol. The can be grouped into two groups that display large differences in selectivity and peak shape due to hydrophobicity, degree of surface coverage silanol activity. Measurements particle size distributions were made using automated microscopy electrical sensing zone measurements. Only a weak correlation could found between efficiency size. Large column...