A5038876397- Genetics, Aging, and Longevity in Model Organisms
- DNA Repair Mechanisms
- Lysosomal Storage Disorders Research
- Colorectal Cancer Treatments and Studies
- Cancer Genomics and Diagnostics
- Genomics, phytochemicals, and oxidative stress
- Gastric Cancer Management and Outcomes
- Mitochondrial Function and Pathology
- Biomedical Research and Pathophysiology
- Calcium signaling and nucleotide metabolism
- Epigenetics and DNA Methylation
- Metabolism, Diabetes, and Cancer
- CRISPR and Genetic Engineering
- bioluminescence and chemiluminescence research
- Redox biology and oxidative stress
- Radiation Therapy and Dosimetry
- Glutathione Transferases and Polymorphisms
- Boron Compounds in Chemistry
- Nuclear Structure and Function
- Advanced biosensing and bioanalysis techniques
- Glycogen Storage Diseases and Myoclonus
- Genetic and rare skin diseases.
- Spaceflight effects on biology
- Particle physics theoretical and experimental studies
- Nuclear Physics and Applications
Kawasaki Medical School
2021-2024
Nagasaki University
2019-2023
University of Tsukuba
1999-2022
Kyoto University
2012-2021
Kyoto University Research Reactor Institute
2016-2017
Toyota Memorial Hospital
2004
University of California, Los Angeles
2001
The lysosome is an acid organelle that contains a variety of hydrolytic enzymes and plays significant role in intracellular degradation to maintain cellular homeostasis. Genetic variants lysosome-related genes can lead severe congenital diseases, such as lysosomal storage diseases. In the present study, we investigated impact depleting lipase A (LIPA), esterase metabolizes esterified cholesterol or triglyceride, on function. Under nutrient-rich conditions, LIPA gene knockout (LIPAKO) cells...
Human oxidation resistance 1 (OXR1) functions in protection against oxidative damage and its homologs are highly conserved eukaryotes examined so far, but function still remains uncertain. In this study, we identified a homolog (LMD-3) of human OXR1 the nematode Caenorhabditis elegans (C. elegans). The expressed LMD-3 was able to suppress mutator phenotypes E. coli mutMmutY mutT mutants. Purified did not have enzymatic activity 8-oxoG, superoxide dismutase (SOD), or catalase activities....
Abstract Vesicular transport driven by membrane trafficking systems conserved in eukaryotes is critical to cellular functionality and homeostasis. It known that homotypic fusion vacuole protein sorting (HOPS) class C core endosomal tethering (CORVET) interact with Rab-GTPases SNARE proteins regulate vesicle transport, fusion, maturation autophagy endocytosis pathways. In this study, we identified two novel “Hybrid” complexes mammalian cells which one of the subunits HOPS or CORVET replaced...
Excessive generation of reactive oxygen species within cells results in oxidative stress. Furthermore, accumulation has been shown to reduce cell longevity. Many dietary supplements are believed have anti-aging effects. The herb mixture KPG-7 contains several components with antioxidant activity. We aim clarify the mechanisms responsible for activity and establish whether an effect. examined supplementation could provide protection against stress, extend lifespan, delay aging Caenorhabditis...
Tirapazamine (TPZ) is an anticancer drug with highly selective cytotoxicity toward hypoxic cells. TPZ converted to a radical intermediate under conditions, and this interacts intracellular macromolecules, including DNA. has been reported indirectly induce DNA double-strand breaks (DSBs) through the formation of various lesions conditions. Although topoisomerase II-DNA complex identified as one these intermediates, other have not yet defined. In order obtain deeper understanding mechanisms...
Abstract Background Inflammation has been demonstrated to promote cancer metastasis. Due the well-known systemic inflammatory responses (SIR) after major surgery, it is critical investigate and attenuate SIR-induced tumor metastasis of patients suffering surgical procedures. Methods C57BL/6 mice were intravenously injected with Lewis lung cells at 6, 24, 72 h induction intestinal ischemia/reperfusion (I/R) injury. We found that number nodules significantly increased in lungs 6 but not 24 I/R...
Mucopolysaccharidoses (MPS) are rare lysosomal storage disorders (LSD) characterized by the excessive accumulation of glycosaminoglycans (GAG). Conventional MPS, caused inborn deficiencies enzymes involved in GAG degradation, display various multisystemic symptoms-including progressive neurological complications, ophthalmological disorders, hearing loss, gastrointestinal and hepatobiliary issues, cardiorespiratory problems, bone joint abnormalities, dwarfism, coarse facial features....
We report a measurement of charge symmetry breaking in the NNη system. have measured ratio differential cross sections charge-symmetric reactions π+d→ppη and π−d→nnη energy region η threshold. Our result is R≡dσ(π+d→ppη)/dσ(π−d→nnη)=0.938±0.009 after phase-space correction made for difference threshold energies two reactions. The deviation R from unity an indication breaking, which mostly due to π0−η mixing. A theoretical model production includes mixing was used fit data yields angle...
Mucopolysaccharidosis-plus syndrome (MPSPS) is an autosomal-recessive disorder caused by c.1492C>T (p.R498W) in the VPS33A gene. MPSPS a severe that causes short lifespan patients. Currently, there no specific treatment for The Yakut population more prone to this disease than others. Diagnosing relies on clinical manifestations, and genetic testing (GT) used confirm diagnosis. In research, we examined two pregnancy cases, one of which involved prenatal diagnosis MPSPS. Notably, neither...
DNA mismatch repair (MMR) contributes to genome integrity by correcting errors of polymerase and inducing cell death in response damage. Dysfunction MMR results increased mutation frequency cancer risk. Clinical researches revealed that abnormalities induce cancers non-dividing tissues, such as kidney liver. However, how suppresses tissues is not understood. To address mechanism, we analyzed the roles cells using Caenorhabditis elegans (C. elegans), which all somatic are adult stage. In this...
Abstract Coffin-Siris syndrome (CSS) is a congenital disorder that characterized by an absent/hypoplastic fifth distal phalanx, psychomotor developmental delay, and coarse facial features. One of the causative genes, ARID1B (AT-rich interactive domain-containing protein 1B), encodes components BAF chromatin remodeling complexes. Here, we report case 3-year 8-month-old male with novel nonsense variant (NM_001374820.1:c.4282C > T, p.(Gln1428*)) in gene, which was identified whole-exome...
Purpose To evaluate the effect of oxygen pressure during incubation with a 10B-carrier on 10B uptake capacity cultured p53 wild-type and mutated tumor cells.Materials methods Cultured human head neck squamous cell carcinoma line transfected mutant TP53 (SAS/mp53), or neo vector as control (SAS/neo) was incubated L-para-boronophenylalanine-10B (BPA) sodium mercaptoundecahydrododecaborate-10B (BSH) at concentration 60 ppm for 24 h under aerobic (20.7% oxygen) hypoxic (0.28% conditions....
Metformin is a biguanide drug that widely used in the treatment of diabetes. Epidemiological studies have indicated metformin exhibits anti-cancer activity. However, molecular mechanisms underlying this activity currently remain unclear. We hypothesized cytotoxic tumor-specific environment such as glucose deprivation and/or low oxygen (O2) tension. herein demonstrated was highly under glucose-depleted, but not hypoxic (2% O2) conditions. In order to elucidate selective cytotoxicity, we...
Introduction . Ataxia‐telangiectasia‐mutated (ATM) kinase is a master regulator of the DNA damage response and directly activated by reactive oxygen species (ROSs) in addition to double‐stranded breaks. However, physiological function ROSs not understood. Purpose In present study, we investigated how ATM responds Caenorhabditis elegans (C. elegans) Materials Methods First, measured sensitivities larvae DNA‐damaging agents ROSs. Next, analyzed drug fully matured adult worms, which consist...
Radiation damages many cellular components and disrupts functions, was previously reported to impair locomotion in the model organism Caenorhabditis elegans. However, response even higher doses is not clear. First, investigate effects of high-dose radiation on C. elegans, we investigated dose range that reduces whole-body or leads death. Irradiation performed 0–6 kGy. In crawling analysis, motility decreased after irradiation a dose-dependent manner. Exposure 6 kGy affected agar immediately...
Oxidatively damaged bases in DNA can cause cell death, mutation and/or cancer induction. To overcome such deleterious effects of base oxidation, cells are equipped with excision repair (BER) initiated by glycosylases. Endonuclease III (Nth), a major glycosylase, mainly excises oxidatively pyrimidines from DNA. The aims this study were to obtain an overview the mechanism and elucidate function BER maintaining genome stability during embryogenesis development. In study, we used ascidian Ciona...
Abstract Background Peroxiredoxin 1 (PRDX1) is a member of ubiquitous family thiol peroxidases that catalyze the reduction peroxides, including hydrogen peroxide. It functions as an antioxidant enzyme, similar to catalase and glutathione peroxidase. PRDX1 was recently shown act sensor reactive oxygen species (ROS) play role in ROS-dependent intracellular signaling pathways. To investigate its physiological functions, conditionally disrupted chicken DT40 cells present study. Results The...
DNA-protein cross-links (DPCs) are generated by internal factors such as cellular aldehydes that during normal metabolism and external environmental mutagens. A nucleoside analog, 5-aza-2'-deoxycytidine (5-azadC), is randomly incorporated into the genome DNA replication binds methyltransferase 1 (DNMT1) covalently to form DNMT1-DPCs without inducing strand breaks. Despite recent progress in understanding mechanisms of DPCs repair, how repaired unclear. The metalloprotease SPRTN has been...
Abstract Background It is well known that kidney injury vital organ damage in Fabry disease (FD). Renin–angiotensin system (RAS) inhibitors are to reduce proteinuria patients with chronic (CKD) by dilating the glomerular export arteries and reducing intraglomerular pressure. This improvement pressure, although lowering filtration rate, thought prevent renal be renoprotective long term. RAS may effective FD progression of disease, however, degree which they used clinical practice unknown....
Abstract We report a Japanese patient with tall stature, dolichocephaly, prominent forehead, narrow nasal ridge, mild retrognathia, subcutaneous fat reduction, bilateral entropion of both eyelids, high arched palate, long fingers, and hyperextensible finger joints as case Marfanoid-progeroid-lipodystrophy syndrome. Genetic investigation revealed heterozygous variant NC_000015.10(NM_000138.5):c.8226+5G>A in the FBN1 gene. Skipping exon 65 escaping nonsense-mediated decay followed by...