A5067471720- Cardiomyopathy and Myosin Studies
- Cardiovascular Effects of Exercise
- Genetics and Physical Performance
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Genetics and Biotechnology
- Heat shock proteins research
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- Protein Structure and Dynamics
- Force Microscopy Techniques and Applications
- Nuclear Structure and Function
Max Perutz Labs
2014-2020
University of Vienna
2014-2020
Vienna Biocenter
2014
Cervantes Institute
2008
University of Crete
2008
The spectrin superfamily of proteins plays key roles in assembling the actin cytoskeleton various cell types, crosslinks filaments, and acts as scaffolds for assembly large protein complexes involved structural integrity mechanosensation, well signaling. α-actinins particular are major crosslinkers muscle Z-disks, focal adhesions, stress fibers. We report a complete high-resolution structure 200 kDa α-actinin-2 dimer from striated explore its functional implications on biochemical cellular...
Gene clusters encoding various type III secretion system (T3SS) injectisomes, frequently code downstream of the conserved atpase gene for small hydrophilic proteins whose amino acid sequences display a propensity intrinsic disorder and coiled-coil formation. These properties were confirmed experimentally member this class, HrpO protein from T3SS Pseudomonas syringae pv phaseolicola: exhibits high alpha-helical content with characteristics, strikingly low melting temperature, structural that...
Significance Actin is one of the most abundant proteins in eukaryotic cells. filaments together with a large number actin-binding are critical players many cellular functions, ranging from cell motility and muscle contraction to maintenance shape transcription regulation. α-Actinin—a member spectrin superfamily—is an archetypical F-actin–binding –bundling protein. It known that Ca 2+ inhibits α-actinin capacity bundle F-actin. We present structure -regulated propose mechanism for its uncover...
α-Actinin is the major component of Z-disk, where it cross-links actin filaments from adjacent sarcomeres. It an antiparallel dimer 200 kDa, containing in each subunit N-terminal binding domain (ABD), a central rod assembled spectrin-like repeats that mediate assembly, and C-terminal calmodulin-like (CaM-like) with 4 EF-hand motifs. Additionally to filaments, α-actinin binds multiple other cytoskeletal signalling proteins. In striated muscle, tightly defined numbers crosslinks between at...