A5077987869- DNA Repair Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Chromatin Dynamics
- Photosynthetic Processes and Mechanisms
- Microtubule and mitosis dynamics
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Effects of Radiation Exposure
- Carcinogens and Genotoxicity Assessment
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- Parasitic Diseases Research and Treatment
- Parasitic Infections and Diagnostics
- Parasite Biology and Host Interactions
- Wheat and Barley Genetics and Pathology
- Insect Resistance and Genetics
- Developmental Biology and Gene Regulation
- Plant Genetic and Mutation Studies
- Fungal and yeast genetics research
- Plant Molecular Biology Research
Stanford University
2013-2022
Sorbonne Université
2013-2016
Université Paris Sciences et Lettres
2016
Institut Curie
2010-2016
Centre National de la Recherche Scientifique
2010-2013
During meiotic prophase, a structure called the synaptonemal complex (SC) assembles at interface between aligned pairs of homologous chromosomes, and crossover recombination events occur their DNA molecules. Here we investigate inter-relationships these two hallmark features program in nematode C. elegans, revealing dynamic properties SC that are modulated by recombination. We demonstrate incorporates new subunits switches from more highly dynamic/labile state to stable as germ cells...
It is widely accepted that morphogenetic gradients determine cell identity by concentration-dependent activation of target genes. How precise each step in the gene expression process acts downstream morphogens, however, remains unclear. The Bicoid morphogen a transcription factor directly activating its genes and provides thus simple system to address this issue quantitative manner. Recent studies indicate gradient precisely established Drosophila embryos after eight nuclear divisions (cycle...
During meiosis, chromosomes adopt a specialized organization involving assembly of cohesin-based axis along their lengths, with DNA loops emanating from this axis. We applied novel, quantitative, and widely applicable cytogenetic strategies to elucidate the molecular bases using Caenorhabditis elegans. Analyses wild-type (WT) de novo circular minichromosomes revealed that meiosis-specific HORMA-domain proteins assemble into cohorts in defined numbers co-organize together 2 functionally...
Meiotic recombination plays dual roles in the evolution and stable inheritance of genomes: Recombination promotes genetic diversity by reassorting variants, it establishes temporary connections between pairs homologous chromosomes that ensure their future segregation. is initiated generation double-strand DNA breaks (DSBs) conserved topoisomerase-like protein Spo11. Despite strong conservation Spo11 across eukaryotic kingdoms, auxiliary complexes interact with to promote DSB formation are...
Significance Double-strand breaks (DSBs) are deleterious DNA lesions, and impairment of the DSB repair machinery can lead to devastating diseases, such as Nijmegen Breakage Syndrome (NBS). During meiosis, DSBs represent a “necessary evil”: they required promote formation crossovers between homologous chromosomes. Crossovers, in turn, ensure correct chromosome inheritance during gamete formation, which is essential for viability normal development embryos. numerous actively created, so...
Abstract Meiotic recombination is initiated by the programmed induction of double-strand DNA breaks (DSBs), lesions that pose a potential threat to genome. A subset DSBs induced during meiotic prophase become designated be repaired pathway specifically yields interhomolog crossovers (COs), which mature into chiasmata temporarily connect homologs ensure their proper segregation at meiosis I. The remaining must other mechanisms restore genomic integrity prior divisions. Here we show HIM-6,...
Meiotic chromosome segregation requires pairwise association between homologs, stabilized by the synaptonemal complex (SC). Here, we investigate factors contributing to synapsis investigating meiosis in polyploid worms. We devised a strategy, based on transient inhibition of cohesin function, generate derivatives virtually any Caenorhabditis elegans strain. exploited this strategy contribution recombination tetraploid and triploid In otherwise wild-type polyploids, chromosomes first sort...
Reduction in ploidy to generate haploid gametes during sexual reproduction is accomplished by the specialized cell division program of meiosis. Pairing between homologous chromosomes and assembly synaptonemal complex at their interface (synapsis) represent intermediate steps meiotic that are essential form crossover recombination-based linkages homologs, which turn enable segregation homologs opposite poles meiosis I division. Here, we challenge mechanisms pairing synapsis C. elegans...
Proper partitioning of homologous chromosomes during meiosis relies on the coordinated execution multiple interconnected events: Homologs must locate, recognize, and align with their correct pairing partners. Further, homolog be coupled to assembly synaptonemal complex (SC), a meiosis-specific tripartite structure that maintains stable associations between axes aligned homologs regulates formation crossovers DNA molecules create linkages enable segregation. Here, we identify HAL-3 (Homolog...
In eukaryotic cells, the organization of genomic DNA into chromatin regulates many biological processes, from control gene expression to regulation chromosome segregation. The proper maintenance this structure upon cell division is therefore prime importance during development for identity and genome stability. assembly factor 1 (CAF-1) involved in H3-H4 histone dimers on newly synthesized a higher order structure, heterochromatin, through an interaction its large subunit with...
Abstract During meiotic prophase, a structure called the synaptonemal complex (SC) assembles at interface between aligned pairs of homologous chromosomes, and crossover recombination events occur their DNA molecules. Here we investigate inter-relationships these two hallmark features program in nematode C. elegans , revealing dynamic properties SC that are modulated by recombination. We demonstrate incorporates new subunits switches from more highly dynamic/labile state to stable as germ...
ABSTRACT During meiosis, chromosomes adopt a specialized organization involving assembly of cohesin-based axis along their lengths, with DNA loops emanating from this axis. We applied novel, quantitative and widely applicable cytogenetic strategies to elucidate the molecular bases using C. elegans . Analyses WT de novo circular mini-chromosomes revealed that meiosis-specific HORMA-domain proteins assemble into cohorts in defined numbers co-organize together two functionally-distinct cohesin...
Abstract Faithful inheritance of genetic information through sexual reproduction relies on the formation crossovers between homologous chromosomes during meiosis, which in turn and repair numerous double-strand DNA breaks (DSBs). As DSBs pose a potential threat to genome, mechanisms that ensure timely error-free DSB are crucial for successful meiosis. Here we identify NBS-1, Caenorhabditis elegans ortholog NBS1 subunit conserved MRE11-RAD50-NBS1/Xrs2 (MRN) complex, as key mediator via...
Abstract Meiotic recombination plays dual roles in the evolution and stable inheritance of genomes: promotes genetic diversity by reassorting variants, it establishes temporary connections between pairs homologous chromosomes that ensure for their future segregation. is initiated generation double-strand DNA breaks (DSBs) conserved topoisomerase-like protein Spo11. Despite strong conservation Spo11 across eukaryotic kingdoms, auxiliary complexes interact with to promote DSB formation are...
Abstract Proper partitioning of homologous chromosomes during meiosis relies on the coordinated execution multiple interconnected events: Homologs must locate, recognize and align with their correct pairing partners. Further, homolog be coupled to assembly synaptonemal complex (SC), a meiosis-specific tripartite structure that maintains stable associations between axes aligned homologs regulates formation crossovers DNA molecules create linkages enable segregation. Here we identify HAL-3 ( H...
A complex series of interconnected events during meiotic prophase creates the physical connections between homologous chromosomes essential to ensure their proper partitioning first division. HIM-19 is an important factor that regulates progression in C. elegans , but its molecular function(s) and localization have remained unclear. We show here tagged expressed from endogenous locus exhibits dynamic germ cell nuclei support proposed role as a regulator CHK-2 protein kinase.