A5082240607- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Aquaculture disease management and microbiota
- Plant Virus Research Studies
- Crustacean biology and ecology
- Biological Research and Disease Studies
- Bacteriophages and microbial interactions
- Aquaculture Nutrition and Growth
- Coastal wetland ecosystem dynamics
Universiti Putra Malaysia
2016-2018
Serdang Hospital
2017
Macrobrachium rosenbergii nodavirus (MrNV) is a pathogen of freshwater prawns that poses threat to food security and causes significant economic losses in the aquaculture industries many developing nations. A detailed understanding MrNV virion structure will inform development strategies control outbreaks. The capsid has also been engineered display heterologous antigens, thus knowledge its atomic resolution benefit efforts develop tools based on this platform. Here, we present an...
Abstract White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses shrimp farms and hatcheries poses a threat to food-security many developing countries. Outbreaks of nodavirus ( Mr NV), causative agent white (WTD) are associated with up 100% mortality rates. There no interventions available treat or prevent NV however. Here we show structure virus-like particles (VLPs) produced by recombinant expression capsid protein, using cryogenic...
Macrobrachium rosenbergii nodavirus (MrNV) is a virus native to giant freshwater prawn. Recombinant MrNV capsid protein has been produced in Escherichia coli, which self-assembled into virus-like particles (VLPs). However, this recombinant unstable, degrading and forming heterogenous VLPs. In study, was insect Spodoptera frugiperda (Sf9) cells through baculovirus system. Dynamic light scattering (DLS) transmission electron microscopy (TEM) revealed that the by highly stable, homogenous VLPs...
Abstract Macrobrachium rosenbergii nodavirus ( Mr NV) is a pathogen of freshwater prawns that poses threat to food-security and causes significant economic losses in the aquaculture industries many developing nations. A detailed understanding NV virion structure will inform development strategies control outbreaks. The capsid has also been engineered display heterologous antigens, thus knowledge its atomic resolution benefit efforts develop tools based on this platform. Here we present an...
Macrobrachium rosenbergii nodavirus (MrNv) causes white tail disease (WTD) in giant freshwater prawns, which leads to devastating economic losses the aquaculture industry. Despite extensive research on MrNv, there is still no antiviral agent treat WTD. Thus, main aim of this study was identify potential anti-MrNv molecules. A 12-mer phage-displayed peptide library biopanned against MrNv virus-like particle (VLP). After four rounds biopanning, two dominant phages harbouring amino acid...
Abstract White tail disease in the giant freshwater prawn Macrobrachium rosenbergii causes significant economic losses shrimp farms and hatcheries poses a threat to food-security many developing countries. Outbreaks of nodavirus ( Mr NV), causative agent white (WTD) are associated with up 100% mortality rates. Recombinant expression capsid protein NV insect cells leads production VLPs closely resembling native virus. We have investigated structure by cryogenic electron microscopy,...