A5032835961- Biosensors and Analytical Detection
- Pancreatic and Hepatic Oncology Research
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Pancreatitis Pathology and Treatment
- SARS-CoV-2 detection and testing
- Nanoparticle-Based Drug Delivery
- COVID-19 Clinical Research Studies
- Gold and Silver Nanoparticles Synthesis and Applications
- SARS-CoV-2 and COVID-19 Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Chemical Sensor Technologies
- Pancreatic function and diabetes
- Spectroscopy Techniques in Biomedical and Chemical Research
Purdue University West Lafayette
2019-2021
While identifying acute HIV infection is critical to providing prompt treatment HIV-positive individuals and preventing transmission, existing laboratory-based testing methods are too complex perform at the point of care. Specifically, molecular techniques can detect RNA within 8-10 days transmission but require laboratory infrastructure for cold-chain reagent storage extensive sample preparation performed by trained personnel. Here, we demonstrate our point-of-care microfluidic rapid...
Reusable, low-power, low-cost, printed microheaters developed for practical use in in-field and point-of-care diagnostics.
Two-dimensional paper networks (2DPNs) have enabled the use of paper-based platforms to perform multistep immunoassays for detection pathogenic diseases at point-of-care. To date, however, has required user provide multiple signal enhancement solutions and been limited protein targets. We solve these challenges by using mathematical equations guide device design a novel 2DPN, which leverages fluidic inputs apply fully dried hydrogen peroxide, diaminobenzidine, horseradish peroxidase reagents...
Abstract Early Human Immunodeficiency Virus (HIV) testing is critical to preventing transmission and providing treatment HIV-positive individuals, yet an estimated 30% of individuals do not know their status because barriers early diagnosis. Readily accessible, highly sensitive, rapid diagnostic tests would enable patients’ prompt with anti-retroviral therapies reduce transmission. However, existing HIV technologies either detect stages infection or require multiple days laboratory...
The classification of pancreatic cyst fluids can provide a basis for the early detection cancer while eliminating unnecessary procedures. A candidate biomarker, gastricsin (pepsin C), was found to be present in potentially malignant mucinous fluids. activity assay using magnetic bead-based platform has been developed immobilized peptide substrates selective bearing dimeric rhodamine dye. unique dye structure allows quantitation enzyme-cleaved product by both fluorescence and surface enhanced...
617 Background: Pancreatic cysts are rarely symptomatic, and, consequently, detected incidentally during routine imaging analyses for unrelated disorders. Guidelines diagnosis of pancreatic rely on and supportive fluid extracted endoscopic ultrasound with fine-needle aspiration (EUS-FNA). Unfortunately, currently available protein markers assays not sufficiently accurate to rule-out patients in whom a non-mucinous cyst has formed. Since seldom progress cancer, this patient population is at...
With the current state of COVID-19 changing from a pandemic to being more endemic, priorities diagnostics will likely vary rapid detection stratification for treatment most vulnerable patients. Such patient can be facilitated using multiple markers, including SARS-CoV-2-specific viral enzymes, like 3CL protease, and viral-life-cycle-associated host proteins, such as ACE2. To enable future explorations, we have developed fluorescent Raman spectroscopic SARS-CoV-2 protease assay that run...
The incidental detection of pancreatic cysts, an opportunity for the early cancer, is increasing, owing to aging population and improvements in imaging technology. classification cystic precursors currently relies on cyst fluid evaluations, including cytology protein genomic analyses. However, there are persistent limitations that obstruct accuracy quality information clinicians, limited volume complex, often acellular, proteinaceous milieu comprises fluid. constraints available clinical...
Abstract Background Current efforts to expand the pool of available markers that can be analyzed in clinical settings is hampered by limited volumes biomaterial-rich fluids and low concentrations certain analytes, coupled with poor sensitivity existing detection techniques. Although progress has been made using advanced sample preparation or assay techniques such as miRNeasy ELISA for genomic protein assays, respectively, little research focused on adapting new optical methods modes clinic....
4151 Background: One impact of medical imaging technology has been an approximately 3-fold increase in the incidental detection pancreatic cysts during routine clinical examinations. To reduce burden on healthcare system and patients, clinicians desire accurate classification into benign non-mucinous or potentially malignant, mucinous populations. Using EUS-FNA, fluid from these can provide molecular biomarkers predictive value. However, current vitro diagnostics lack desired sensitivity...