A5023595240- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Marine and coastal ecosystems
- Microbial Metabolic Engineering and Bioproduction
- Microbial Community Ecology and Physiology
- Diatoms and Algae Research
- Plant Reproductive Biology
- Photoreceptor and optogenetics research
- Advanced Proteomics Techniques and Applications
- GABA and Rice Research
- Plant Molecular Biology Research
- Insect-Plant Interactions and Control
- Marine Bivalve and Aquaculture Studies
- Plant Virus Research Studies
- Magnetic and Electromagnetic Effects
- Spectroscopy and Quantum Chemical Studies
- Molecular spectroscopy and chirality
- Marine and coastal plant biology
- Plant Stress Responses and Tolerance
- Calcium Carbonate Crystallization and Inhibition
- Agriculture, Soil, Plant Science
- Thermodynamic properties of mixtures
- Antioxidant Activity and Oxidative Stress
- Molecular Junctions and Nanostructures
Chinese Academy of Sciences
2016-2024
Institute of Hydrobiology
2016-2024
Wuhan University
2009-2016
Guangdong Pharmaceutical University
2007-2014
Brown planthopper (BPH), Nilaparvata lugens Stål, is one of the most devastating insect pests rice (Oryza sativa L.). Currently, 30 BPH-resistance genes have been genetically defined, which are clustered on specific chromosome regions. Here, we describe molecular cloning and characterization a gene, BPH9, mapped long arm 12 (12L). BPH9 encodes rare type nucleotide-binding leucine-rich repeat (NLR)-containing protein that localizes to endomembrane system causes cell death phenotype. activates...
Photosynthetic oleaginous microalgae are considered promising feedstocks for biofuels. The marine microalga, Nannochloropsis oceanica, has been attracting ever-increasing interest because of its fast growth, high triacylglycerol (TAG) content, and available genome sequence genetic tools. Diacylglycerol acyltransferase (DGAT) catalyzes the last committed step TAG biosynthesis in acyl-CoA-dependent pathway. Previous studies have identified 13 putative DGAT-encoding genes N. but functional role...
Brown planthopper (BPH, Nilaparvata lugens Stål), is the most destructive phloem-feeding insect pest of rice (Oryza sativa). The BPH-resistance gene BPH15 has been proved to be effective in controlling and widely applied breeding programs. Nevertheless, molecular mechanism resistance remain unclear. In this study, we narrowed down position on chromosome 4 investigated transcriptome after BPH attacked. We analyzed 13,000 BC2F2 plants cross between susceptible TN1 recombinant inbred line RI93...
Abstract Background Fucoxanthin has been widely investigated owing to its beneficial biological properties, and the model diatom Phaeodactylum tricornutum , possessing fucoxanthin (Fux) chlorophyll proteins as light-harvesting systems, is considered have potential become a commercial cell factory for pigment production. Results Here, we compared contents in 10 different P. strains from globe, found that strain CCMP631 (Pt6) exhibited highest Fux content but with low biomass. Comparison of...
In photosynthetic oleaginous microalgae, acyl-CoA molecules are used as substrates for the biosynthesis of membrane glycerolipids, triacylglycerol (TAG) and other acylated molecules. Acyl-CoA can also be directed to beta-oxidative catabolism. They utilized by a number lipid metabolic enzymes including endogenous thioesterases, which catalyze their hydrolysis release free fatty acids. availability thus plays fundamental roles in determining quantity composition lipids storage lipids. Here, we...
Triacylglycerols (TAGs) are the main storage lipids in photosynthetic organisms under stress. In oleaginous alga Nannochloropsis oceanica, while multiple acyl CoA:diacylglycerol (DAG) acyltransferases (NoDGATs) involved TAG production, role of unique phospholipid:DAG acyltransferase (NoPDAT) remains unknown. Here, we performed a functional complementation assay TAG-deficient yeast (Saccharomyces cerevisiae) and an vitro to probe activity NoPDAT. Subcellular localization, overexpression,...
Abstract Background The marine alga Nannochloropsis oceanica , an emerging model belonging to Heterokont, is considered as a promising light-driven eukaryotic chassis for transforming carbon dioxide various compounds including carotenoids. Nevertheless, the carotenogenic genes and their roles in remain less understood be further explored. Results Here, two phylogenetically distant zeaxanthin epoxidase (ZEP) from N. ( NoZEP1 NoZEP2 ) were functionally characterized. Subcellular localization...
Glycine decarboxylase complex (GDC) is a multi-protein complex, comprising P-, H-, T- and L-protein subunits, which plays major role in photorespiration plants. While structural analysis has demonstrated that the H subunit of GDC (GDCH) pivotal GDC, research on GDCH biological processes plants seldom reported. Here, function GDCH, stresses resulting from GDCH-knockdown interactions these with other cellular were studied rice Under high CO(2), OsGDCH RNA interference (OsGDCH-RNAi) grew...
SUMMARY Phaeodactylum tricornutum plastid is surrounded by four membranes, and its protein composition function remain mysterious. In this study, the P. plastid‐enriched fraction was obtained 2850 proteins were identified, including 92 plastid‐encoded proteins, through label‐free quantitative proteomic technology. Among them, 839 nuclear‐encoded further determined to be plastidial based on BLAST alignments within Plant Proteome DataBase subcellular localization prediction, in spite of strong...
Summary Nannochloropsis oceanica is an industrially relevant marine microalga rich in eicosapentaenoic acid (EPA, a valuable ω‐3 polyunsaturated fatty acid), yet the algal production potential remains to be unlocked. Here we engineered N. synthesize high‐value carotenoid astaxanthin independent of high‐light (HL) induction for achieving multifaceted benefits. By screening β‐carotenoid ketolases and hydroxylases various origins, strategically manipulating compartmentalization, fusion...
Abstract Oleaginous algae have the ability to synthesize a high level of triacylglycerol (TAG) and are considered as next‐generation feedstock for biofuel production. Manipulating algal lipid biosynthetic pathways has potential overproduce TAG represents feasible way toward bringing down algae‐derived biofuels production cost. Here we dissected functional roles engineering six diacylglycerol acyltransferase (DGAT) genes from marine alga Phaeodactylum tricornutum . PtDGAT1, PtDGAT2B, PtDGAT3,...
Nε-lysine acetylation represents a highly dynamic and reversibly regulated post-translational modification widespread in almost all organisms, plays important roles for regulation of protein function diverse metabolic pathways. However, little is known about the role lysine photosynthetic eukaryotic microalgae. We integrated proteomic approaches to comprehensively characterize acetylome model diatom Phaeodactylum tricornutum In total, 2324 sites from 1220 acetylated proteins were identified,...
Nannochloropsis oceanica represents a promising sunlight-driven alga for producing eicosapentaenoic acid (EPA, 20:5Δ5,8,11,14,17), value-added very long-chain polyunsaturated fatty (VLC-PUFA). Here, we unraveled the function and roles of Δ6 elongase (NoΔ6-FAE) in N. oceanica. Heterologous expression NoΔ6-FAE yeast confirmed its elongating C18 Δ6-PUFAs rather than others. Subcellular localization experiments suggested that resides chloroplast endoplasmic reticulum. knockdown attenuated...
Summary Diatoms are successful phytoplankton clades able to acclimate changing environmental conditions, including e.g. variable light intensity. outstanding at dissipating energy exceeding the maximum photosynthetic electron transfer (PET) capacity via nonphotochemical quenching (NPQ) process. While molecular effectors of NPQ as well involvement proton motive force (PMF) in its regulation known, regulators PET/PMF relationship remain unidentified diatoms. We generated mutants H + /K...
Abstract In the acyl-CoA-independent pathway of triacylglycerol (TAG) synthesis unique to plants, fungi, and algae, TAG formation is catalyzed by enzyme phospholipid:diacylglycerol acyltransferase (PDAT). The PDAT gene model diatom Phaeodactylum tricornutum strain CCMP2561 boasts 47 single nucleotide variants within protein coding regions alleles. To deepen our understanding synthesis, we observed allele-specific expression analysis 87 published RNA-sequencing (RNA-seq) data experimental...
Thermosensitive genic male sterile (TGMS) lines and photoperiod-sensitive (PGMS) have been successfully used in hybridization to improve rice yields. However, the molecular mechanisms underlying sterility transitions most PGMS/TGMS are unclear. In recently developed TGMS-Co27 line, is based on co-suppression of a UDP-glucose pyrophosphorylase gene (Ugp1), but further study needed fully elucidate involved. Microarray-based transcriptome profiling wild-type Hejiang 19 (H1493) plants grown at...
Since methylmalonyl-CoA epimerase appears to be absent in the majority of photosynthetic organisms, including diatoms, (S)-methylmalonyl-CoA, intermediate isoleucine (Ile) catabolism, cannot metabolized (R)-methylmalonyl-CoA then succinyl-CoA. In this study, propionyl-CoA carboxylase (PCC) RNAi silenced strains and 3-hydroxyisobutyryl-CoA hydrolase (HIBCH) overexpression were constructed elucidate Ile degradation pathway its influence on lipid accumulation Phaeodactylum tricornutum based...