A5027982315- Electrochemical sensors and biosensors
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Electrochemical Analysis and Applications
- Microbial Fuel Cells and Bioremediation
- Polyamine Metabolism and Applications
- Enzyme Catalysis and Immobilization
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Biochemical and Molecular Research
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Photoreceptor and optogenetics research
- Photosynthetic Processes and Mechanisms
- Biosensors and Analytical Detection
- Pancreatic function and diabetes
- Advanced Nanomaterials in Catalysis
- Genomics and Phylogenetic Studies
- Molecular Junctions and Nanostructures
- Porphyrin Metabolism and Disorders
- Microbial Metabolic Engineering and Bioproduction
- Antimicrobial Resistance in Staphylococcus
- Enzyme-mediated dye degradation
- Microbial metabolism and enzyme function
- Bone and Dental Protein Studies
University of Fukui
2015-2024
Fukuyama University
2004-2015
National Institute of Technology, Yonago College
2008-2010
Tokushima University
2001-2002
A gene encoding an ADP-dependent phosphofructokinase homologue has been identified in the hyperthermophilic archaeon Methanococcus jannaschii via genome sequencing. The encoded a protein of 462 amino acids with molecular weight 53,361. deduced acid sequence showed 52 and 29% identities to glucokinase from Pyrococcus furiosus, respectively. was overexpressed Escherichia coli, produced enzyme purified characterized. To our surprise, high activities for both phosphofructokinase. native mass...
ABSTRACT During DNA microarray analysis, we discovered that the GlnK-GlnL (formerly YcbA-YcbB) two-component system positively regulates expression of glsA-glnT ybgJ-ybgH ) operon in response to glutamine culture medium on Northern analysis. As a result gel retardation and DNase I footprinting analyses, found GlnL protein interacts with region (bases −13 −56; +1 is transcription initiation base determined primer extension analysis glsA - glnT which direct repeat, TTTTGTN4TTTTGT, present....
Branched-chain amino acids are the most abundant in proteins. The Bacillus subtilis ilv-leu operon is involved biosynthesis of branched-chain acids. This exhibits a RelA-dependent positive stringent response to acid starvation. We investigated this upon lysine starvation as well decoyinine treatment. Deletion analysis involving various lacZ fusions revealed two molecular mechanisms underlying ilv-leu, i.e., CodY-dependent and -independent mechanisms. former likely triggered by decrease vivo...
ABSTRACT The Bacillus subtilis fadR regulon involved in fatty acid degradation comprises five operons, lcfA-fadR-fadB-etfB-etfA , lcfB fadN-fadA-fadE fadH-fadG and fadF-acdA-rpoE . Since the lcfA-fadRB-etfBA fadNAE whose gene products directly participate β-oxidation cycle, had been found to be probably catabolite repressed upon genome-wide transcript analysis, we performed Northern blotting, which indicated that they are clearly under CcpA-dependent repression. So, searched for...
The activity of dye-linkedd-proline dehydrogenase was found in the crude extract a hyperthermophilic archaeon, Pyrobaculum islandicum JCM 9189. dye-linked d-proline membrane associated enzyme and solubilized from fractions by treatment with Tween 20. purified 34-fold presence 0.1% 20 four sequential chromatographies. has molecular mass about 145 kDa consisted homotetrameric subunits 42 kDa. N-terminal amino acid sequence subunit MKVAIVGGGIIGLFTAYHLRQQGADVVI. retained its full both after...
The Bacillus subtilis ilv-leu operon is involved in the synthesis of branched-chain amino acids (valine, isoleucine, and leucine). two- to threefold repression expression during logarithmic-phase growth under nitrogen-limited conditions, which was originally detected by a DNA microarray analysis compare transcriptomes from wild-type tnrA mutant strains, confirmed lacZ fusion Northern experiments. A genome-wide TnrA box search revealed candidate approximately 200 bp upstream transcription...
ABSTRACT The Bacillus subtilis lmrAB operon is involved in multidrug resistance. LmrA a repressor of its own operon, while LmrB acts as efflux transporter. was produced Escherichia coli cells and shown to bind the lmr promoter region, which an LmrA-binding site identified. Genome-wide screening involving DNA microarray analysis allowed us conclude that also repressed yxaGH , not likely contribute bound putative two tandem sites were regulon thus determined comprise . All three contained...
l-hydroxyproline (l-Hyp: trans-4-hydroxy-l-proline) is commonly found at high concentrations in connective tissue proteins such as collagen. It a remarkably useful molecular marker because variation the level of l-Hyp associated with various diseases. Recently, novel enzymes epimerase and d-hydroxyproline dehydrogenase were isolated from bacteria. In this study, electrochemical biosensor for was constructed using these two enzymes. epimerized to (d-Hyp: cis-4-hydroxy-d-proline), d-Hyp...
The Bacillus subtilis ilv-leu operon functions in the biosynthesis of branched-chain amino acids. It undergoes catabolite activation involving a promoter-proximal cre which is mediated by complex CcpA and P-Ser-HPr. This expression negatively regulated through CodY binding to high-affinity site promoter region under acid-rich growth conditions, it TnrA box nitrogen-limited conditions. CcpA-mediated required helix face-dependent interaction P-Ser-HPr with RNA polymerase needed 19-nucleotide...
The Bacillus subtilis rhaEWRBMA (formerly yuxG-yulBCDE) operon consists of four genes encoding enzymes for l-rhamnose catabolism and the rhaR gene a DeoR-type transcriptional regulator. DNase I footprinting analysis showed that RhaR protein specifically binds to regulatory region upstream rhaEW gene, in which two imperfect direct repeats are included. Gel retardation revealed repeat farther is essential high-affinity binding DNA was effectively inhibited by L-rhamnulose-1-phosphate, an...
ABSTRACT The distribution of dye-linked l -amino acid dehydrogenases was investigated in several hyperthermophiles, and the activity -proline dehydrogenase (dye- -proDH, -proline:acceptor oxidoreductase) found crude extract some Thermococcales strains. enzyme purified to homogeneity from a hyperthermophilic archaeon, Thermococcus profundus DSM 9503, which exhibited highest specific extract. molecular mass about 160 kDa, consisted heterotetrameric subunits (α 2 β ) with two different masses...
In the present study, a progesterone (P4) biosensor was developed to detect cattle estrus.Thionin, which has been reported oxidize steroid hormones, immobilized on an electrode via 10-carboxy-1-decanethiol with aim of continuous measurement P4 in body.On screen-printed electrode, Au nanoparticles were electrodeposited surface carbon increase area electrode.Finally, thionin-modified covered Nafion TM .As result, influence contaminants (BSA, L-ascorbic acid) avoided.The detection range...