A5026009871- Research on Leishmaniasis Studies
- Parasites and Host Interactions
- Trypanosoma species research and implications
- Congenital Diaphragmatic Hernia Studies
- Dermatological diseases and infestations
- Autoimmune and Inflammatory Disorders Research
- Psoriasis: Treatment and Pathogenesis
- Neonatal Respiratory Health Research
- Infant Nutrition and Health
Fundação Oswaldo Cruz
2013-2022
Universidade Federal de Pernambuco
2015
Leishmaniases are caused by obligate intracellular protozoan parasites of the genus Leishmania. They cause a spectrum diseases, most notably visceral (VL), cutaneous (CL), and mucosal (ML) leishmaniasis, which affect millions people around world, each year. Despite scientific advances, leishmaniases cases expanding, constituting an important public health problem. Immunological molecular diagnostic tools have been increasingly applied for early detection these parasitic infections, since...
Molecular biological methods have become increasingly relevant to the diagnosis and control of infectious diseases, such as leishmaniasis. Since various factors may affect sensitivity PCR assays, including DNA yield purity, an optimal extraction method is pivotal. Losses a parasite’s during significantly impair its detection by lead false-negative results. This study proposes triplex assay targeting DNA, external (pUC18) internal (G3PD) for accurate Two primer pairs were designed detect...
American cutaneous leishmaniosis (ACL) is a disease caused by different species of Leishmania protozoa, braziliensis being the main found in Brail. In this study, two rural areas Pernambuco, northeastern Brazil, where ACL endemic, were selected. Genomic DNA was extracted from canine ectoparasites (ticks, fleas, and lice) tested using conventional PCR quantitative real time PCR. A total 117 collected, 50 (42.74%) them positive for L. (in at least one protocol), with mean parasite load 14.14...
INTRODUCTION: Molecular techniques have been shown to be alternative methods for the accurate detection of infectious and parasitic diseases, such as leishmaniases. The present study describes optimization evaluation a duplex real-time quantitative PCR (qPCR) protocol developed simultaneous Leishmania infantum DNA sample quality control. METHODS: After preliminary tests with newly designed TaqMan® probes two targets ( L. glyceraldehyde 3-phosphate dehydrogenase (G3PD) gene), qPCR was...