A5042788816- Protein Hydrolysis and Bioactive Peptides
- Proteins in Food Systems
- Digestive system and related health
- Biochemical Analysis and Sensing Techniques
- Nutrition, Genetics, and Disease
- Diabetes Treatment and Management
- Microbial Metabolites in Food Biotechnology
- Regulation of Appetite and Obesity
- Food composition and properties
- Neuropeptides and Animal Physiology
- Infant Nutrition and Health
- Obesity, Physical Activity, Diet
- Diet and metabolism studies
- Neuroendocrine Tumor Research Advances
- Food Allergy and Anaphylaxis Research
- Algal biology and biofuel production
- Breastfeeding Practices and Influences
- Ion Transport and Channel Regulation
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Peptides and Activities
- Effects and risks of endocrine disrupting chemicals
- Gastrointestinal motility and disorders
- Diet, Metabolism, and Disease
- Natural Products and Biological Research
University of Cambridge
2024
Wellcome/MRC Institute of Metabolic Science
2023-2024
Addenbrooke's Hospital
2023-2024
Wellcome Trust
2023-2024
University of Limerick
2021-2023
Instituto de Investigación en Ciencias de la Alimentación
2018-2022
Universidad Autónoma de Madrid
2018-2022
Consejo Superior de Investigaciones Científicas
2018
Abstract The impact of growth conditions on the nitrogen, protein, colour and amino acid profiles Palmaria palmata (from 2 locations) were investigated. Laboratory cultures P. grown over a period 18 days with without nutrient addition, i.e., culture medium (F/2) urea (0.05 or 0.10 g L −1 ). total nitrogen (TN) content cultured originating from longlines harvested Cork increased addition ) in comparison to initial sample (4.15 ± 0.05 vs 1.73 0.03%) while biomass Galway (0.10 (3.72 2.43...
The present study evaluates casein and whey protein gastrointestinal digests as inducers of CCK GLP-1 secretion expression in STC-1 cells.
Abstract Aims/hypothesis Glucose-dependent insulinotropic polypeptide (GIP) is an incretin hormone secreted by enteroendocrine K cells in the proximal small intestine. This study aimed to explore function of human at molecular and cellular levels. Methods CRISPR-Cas9 homology-directed repair was used insert transgenes encoding a yellow fluorescent protein (Venus) or Epac-based cAMP sensor (Epac-S-H187) GIP locus duodenal-derived organoids. Fluorescently labelled were purified FACS for...
Background and Aims: Gastrointestinal (GI) enterochromaffin (EC) cells are specialised sensors of luminal stimuli. They secrete most the body's serotonin (5-HT), critical for modulating GI motility, secretion, sensation, while also signalling satiety intestinal discomfort. The aim this study was to investigate mechanisms underlying regulation human EC cells, relative importance direct nutrient stimulation compared with neuronal paracrine regulation. Methods: Intestinal organoids from...
Aim of this work was to evaluate the impact bread enrichment with different concentrations lentil flour (LF) (0 g/100g, 10 g/100g and 20 g/100g) on some physico-chemical, structural, nutritional features bread. The addition LF in formulation affected its properties, leading a darker appearance more compact structure (e.g., higher density lower gas bubble area fraction). Besides, it led significant rise protein, free glucose, rapidly digestible starch content as well expected glycemic index....
The use of enzymes from the brush border membrane (BBM) in simulated gastrointestinal digestion milk proteins has been evaluated. With this purpose, resistant sequences casein and whey after INFOGEST vitro with without BBM have analyzed by tandem mass spectrometry. revealed additional cleavages to those found pancreatic enzymes, although number total identified peptides decreased due reduction peptide size. These new were mainly attributed activity amino- carboxy-peptidases, which was also...
These data are related to the research article entitled "Induction of CCK and GLP-1 release in enteroendocrine cells by egg white peptides generated during gastrointestinal digestion". In this article, peptide free amino acid profile vitro digests is shown. Egg proteins were digested following INFOGEST digestion protocol. Different time points gastric intestinal characterized regarding protein, content. Protein degradation was followed SDS-PAGE where some electrophoretic bands identified...
Gastrointestinal digests of milk and egg white proteins inhibited DPP-IV activity. The absorbable fraction from the intestinal induced insulin secretion in BRIN-BD11 cells.