A5091379038- Metabolism, Diabetes, and Cancer
- Microbial Fuel Cells and Bioremediation
- Ion Transport and Channel Regulation
- Electrochemical sensors and biosensors
- Calcium signaling and nucleotide metabolism
- Metal Extraction and Bioleaching
- Electrochemical Analysis and Applications
- Magnesium in Health and Disease
- Photosynthetic Processes and Mechanisms
- Coding theory and cryptography
- Soil Carbon and Nitrogen Dynamics
- Erythrocyte Function and Pathophysiology
- Radioactive element chemistry and processing
- Plant responses to elevated CO2
- Protein Kinase Regulation and GTPase Signaling
- Plant Growth Enhancement Techniques
The University of Texas Southwestern Medical Center
2023-2024
Southwestern Medical Center
2023-2024
Universidade Nova de Lisboa
2018-2022
Unidade em Ciências Biomoleculares Aplicadas
2022
Rede de Química e Tecnologia
2018
State University of Norte Fluminense
2008
Abstract The apparent high molecular mass of humic acids (HAs) hardly seems compatible with their direct effects in plant physiology. However, previous evidence has indicated that HAs are non‐covalent associations relatively small molecules, which can be broken down by the action organic acids. aim this work was to evaluate on structure spectroscopy and bioactivity following responses maize root growth. Changes exudation from seedlings treated at 50 mg C L −1 were evaluated high‐performance...
Previous study has demonstrated that the WNK kinases 1 and 3 are direct osmosensors consistent with their established role in cell-volume control. may also be regulated by hydrostatic pressure. Hydrostatic pressure applied to cells culture N2 gas or Drosophila Malpighian tubules centrifugation induces phosphorylation of downstream effectors endogenous WNKs. In vitro, autophosphorylation activity unphosphorylated kinase domain WNK3 (uWNK3) is enhanced a lesser extent than 190 kPa gas....
The monoheme outer membrane cytochrome F (OmcF) from Geobacter sulfurreducens plays an important role in Fe(III) reduction and electric current production. electrochemical characterization of this has shown that its redox potential is modulated by the solution pH (redox-Bohr effect) endowing protein with necessary properties to couple electron proton transfer physiological range. analysis OmcF structures reduced oxidized states showed exception side chain histidine 47 (His47), all other...
Osmotic stress and chloride regulate the autophosphorylation activity of WNK1 WNK3 kinase domains. The domain unphosphorylated (uWNK1) is an asymmetric dimer possessing water molecules conserved in multiple uWNK1 crystal structures. Conserved waters are present two networks, referred to here as networks 1 2 (CWN1 CWN2). Here, we show that PEG400 applied crystals dimeric induces de-dimerization. Both chloride-binding site disrupted by PEG400. CWN1 surrounded a cluster pan-WNK-conserved...
Electron harvesting bacteria are key targets to develop microbial electrosynthesis technologies, which valid alternatives for the production of value-added compounds without utilization fossil fuels. Geobacter sulfurreducens, that is capable donating and accepting electrons from electrodes, one most promising electroactive bacteria. Its electron transfer mechanisms electrodes have been progressively elucidated, however pathways still poorly understood. Previous studies showed periplasmic...
Osmotic stress and chloride regulate the autophosphorylation activity of WNK1 WNK3 kinase domains. The domain unphosphorylated (uWNK1) is an asymmetric dimer possessing water molecules conserved in multiple uWNK1 crystal structures. Conserved waters are present two networks, referred to here as networks 1 2 (CWN1and CWN2). Here we show that PEG400 applied crystals dimeric induces de-dimerization. Both binding site disrupted by PEG400. CWN1 surrounded a cluster pan-WNK-conserved charged...
Osmotic stress and chloride regulate the autophosphorylation activity of WNK1 WNK3 kinase domains. The domain unphosphorylated (uWNK1) is an asymmetric dimer possessing water molecules conserved in multiple uWNK1 crystal structures. Conserved waters are present two networks, referred to here as networks 1 2 (CWN1 CWN2). Here, we show that PEG400 applied crystals dimeric induces de-dimerization. Both chloride-binding site disrupted by PEG400. CWN1 surrounded a cluster pan-WNK-conserved...
Geobacter bacteria are able to transfer electrons the exterior of cell and reduce extracellular electron acceptors including toxic/radioactive metals electrode surfaces, with potential applications in bioremediation or electricity harvesting. The triheme c-type cytochrome PpcA from metallireducens plays a crucial role bridging inner outer membrane, ensuring an effective transfer. This shares 80% identity sulfurreducens, but their redox properties markedly different, thus determining...
Osmotic stress and chloride regulate the autophosphorylation activity of WNK1 WNK3 kinase domains. The domain unphosphorylated (uWNK1) is an asymmetric dimer possessing water molecules conserved in multiple uWNK1 crystal structures. Conserved waters are present two networks, referred to here as networks 1 2 (CWN1and CWN2). Here we show that PEG400 applied crystals dimeric induces de-dimerization. Both binding site disrupted by PEG400. CWN1 surrounded a cluster pan-WNK-conserved charged...
Previous observations showed that chloride and osmotic stress regulate the autophosphorylation activity of kinase domains WNK1 WNK3. Further, prior crystallography on asymmetric dimeric unphosphorylated domain (WNK1/S382A, WNK1/SA) revealed conserved waters in active site. Here we show by PEG400 applied to crystals WNK1/SA grown space group P1 induces de-dimerization with a change P2 1 . Both waters, referred here as water network (CWN1) binding site are disrupted PEG400. CWN1 is surrounded...