A5008637894- Cannabis and Cannabinoid Research
- Glycosylation and Glycoproteins Research
- Prenatal Substance Exposure Effects
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pneumocystis jirovecii pneumonia detection and treatment
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Galectins and Cancer Biology
- Pneumonia and Respiratory Infections
- Caveolin-1 and cellular processes
- Ion channel regulation and function
- RNA modifications and cancer
- Immune cells in cancer
- Immune Response and Inflammation
- Phagocytosis and Immune Regulation
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Genetics and Neurodevelopmental Disorders
- Phytoplasmas and Hemiptera pathogens
- Plant tissue culture and regeneration
- Neonatal Respiratory Health Research
- Toxin Mechanisms and Immunotoxins
- Sleep and Wakefulness Research
Eastern Virginia Medical School
2015-2025
Old Dominion University
2025
Nathan Kline Institute for Psychiatric Research
2013-2018
City College of New York
2013-2016
The Ohio State University
2014-2015
Georgia Regents Medical Center
2014
Augusta University
2014
Columbia University
2014
New York State Psychiatric Institute
2014
Karnatak University
2010-2012
ABSTRACT In rodents, many exogenous and endogenous cannabinoids, such as anandamide (AEA) 2‐arachidonyl glycerol (2‐AG), have been shown to play an important role in certain hippocampal memory processes. However, the mechanisms by which AEA regulate this processes are not well understood. Here effects of on long‐term potentiation (LTP), hippocampal‐dependent learning tasks, pERK1/2, pCaMKIV, pCREB signaling events both cannabinoid receptor type 1 (CB1R) wild‐type (WT) knockout (KO) mice were...
The significant consequences of ethanol use during pregnancy are neurobehavioral abnormalities involving hippocampal and neocortex malfunctions that cause learning memory deficits collectively named fetal alcohol spectrum disorder. However, the molecular mechanisms underlying these still poorly understood therefore warrant systematic research. Here, we document novel epigenetic in mouse model Ethanol treatment P7 mice, which induces activation caspase 3, impaired DNA methylation through...
Ethanol exposure to rodents during postnatal day 7 (P7), which is comparable the third trimester of human pregnancy, induces long-term potentiation and memory deficits. However, molecular mechanisms underlying these deficits are still poorly understood. In present study, we explored potential role epigenetic changes at cannabinoid type 1 (CB1R) exon1 additional CB1R functions, could promote in animal models fetal alcohol spectrum disorder. We found that ethanol treatment P7 mice enhances...
Background: Exposure to sulfur mustard (SM; 2,2′-dichlorodiethyl sulfide) causes toxicity in the human body, particularly lungs. The molecular mechanisms of SM-induced lung damage are elusive, and no effective treatments exist. This study explores anti-inflammatory potential cannabinoid receptor 2 (CB2R) activation mitigating acute injury (ALI) inflammation induced by 2-chloroethyl ethyl sulfide (CEES), a structural analog SM. Methods: C57BL/6J mice were exposed CEES via intratracheal...
The molecular mechanisms for lung cell repair are largely unknown. Previous studies identified tripartite motif protein 72 (TRIM72) from striated muscle and linked its function to tissue repair. In this study, we characterized TRIM72 expression in tissues investigated the role of alveolar epithelial cells. vivo injury cells was introduced by high tidal volume ventilation, repair-defective were labeled with postinjury administration propidium iodide. Primary isolated membrane wounding...
Glycan array analysis of Sclerotium rolfsii lectin (SRL) revealed its exquisite binding specificity to the oncofetal Thomsen-Friedenreich (Galβ1-3GalNAcα-O-Ser/Thr, T or TF) antigen and derivatives. This study shows that SRL strongly inhibits growth human colon cancer HT29 DLD-1 cells by cell surface glycans induction apoptosis through both caspase-8 -9 mediated signaling. showed no very weak normal tissues but strong cancerous metastatic tissues. Intratumor injection at subtoxic...
Alcohol exposure can affect brain development, leading to long-lasting behavioral problems, including cognitive impairment, which together is referred as fetal alcohol spectrum disorder (FASD). However, the fundamental mechanisms through this occurs are largely unknown. In study, we report that of postnatal day 7 (P7) mice ethanol activates caspase-3 via cannabinoid receptor type-1 (CB1R) in neonatal mice, and causes a reduction methylated DNA binding protein (MeCP2) levels. The...
The complement system plays a critical role in immune responses against pathogens. However, its identity and regulation the lung are not fully understood. This study aimed to explore of tripartite motif protein (TRIM) 72 regulating receptor (CR) Ig superfamily (CRIg) alveolar macrophage (AM) innate immunity lung. Imaging, absorbance quantification, flow cytometry were used evaluate vitro vivo AM phagocytosis with normal, or altered, TRIM72 expression. Pulldown, coimmunoprecipitation,...
Alveolar epithelial and endothelial cell injury is a major feature of the acute respiratory distress syndrome, in particular when conjunction with ventilation therapies. Previously we showed [Kim SC, Kellett T, Wang S, Nishi M, Nagre N, Zhou B, Flodby P, Shilo K, Ghadiali SN, Takeshima H, Hubmayr RD, Zhao X. Am J Physiol Lung Cell Mol 307: L449–L459, 2014.] that tripartite motif protein 72 (TRIM72) essential for amending alveolar injury. Here, posit TRIM72 improves cellular integrity through...
Ophthalmic mycoses caused by infectious fungi are being recognized as a serious concern since they lead to total blindness. Cephalosporium is one amongst several opportunistic fungal species implicated in ophthalmic infections leading mycotic keratitis. A mitogenic lectin has been purified from the mycelia of fungus Cephalosporium, isolated corneal smears keratitis patient. (CSL) tetramer with subunit mass 14 kDa, agglutinates human A, B, and O erythrocytes, exhibits high affinity for mucin...
Studies showed that TRIM72 is essential for repair of alveolar cell membrane disruptions, and exogenous recombinant human protein (rhT72) demonstrated tissue-mending properties in animal models tissue injury. Here we examine the mechanisms rhT72-mediated lung protection vitro test efficacy inhaled rhT72 reducing pathology a mouse model ventilator-induced In injury was induced by glass beads stretching. Ventilator-induced modeled injurious ventilation at 30 ml/kg tidal volume....
Alveolar macrophages (AMs) guard the alveolar space of lung. Phagocytosis by AMs plays a critical role in defense against invading pathogens, removal dead cells or foreign particles, and resolution inflammatory responses tissue remodeling, processes that are mediated various surface receptors AMs. Here, we report methods for analysis phagocytic function using vitro vivo assays experimental strategies to differentiate between pattern recognition receptor-, complement Fc gamma...
Abstract Background Chronic tissue injury was shown to induce progressive scarring in fibrotic diseases such as idiopathic pulmonary fibrosis (IPF), while an array of repair/regeneration and stress responses come equilibrium determine the outcome at organ level. In lung, type I alveolar epithelial (ATI) cells constitute barrier, II (ATII) play a pivotal role regenerating injured distal lungs. It had been demonstrated that eukaryotic possess repair machinery can quickly patch damaged plasma...
Bacterial pneumonia is a major risk factor for acute lung injury (ALI) and respiratory distress syndrome (ARDS). Pseudomonas aeruginosa (PA), an opportunistic pathogen with increasing resistance acquired against multiple drugs, one of the main causative agents ALI ARDS in diverse clinical settings. Given anti-inflammatory role cannabinoid-2 receptor (CB2R), effect CB2R activation regulation PA-induced inflammation was tested mouse model as alternative to conventional antibiotic therapy.In...
Alveolar macrophages (AMs) guard the alveolar space of lung. Phagocytosis by AMs plays a critical role in defense against invading pathogens, removal dead cells or foreign particles, and resolution inflammatory responses tissue remodeling, processes that are mediated various surface receptors AMs. Here, we report methods for analysis phagocytic function using vitro vivo assays experimental strategies to differentiate between pattern recognition receptor-, complement Fc gamma...
The molecular mechanisms for alveolar epithelial cell plasma membrane wounding and repair are largely unknown. Previous studies identified TRIM72 (also known as mitsugumin 53) from striated muscle tissues linked its physiological functions in these to repair, proliferation, glucose metabolism. Here, using biochemical immunochemical assays, we have characterized expression lung tissue cells, investigated the function of cells. In vivo injury alveolus resident cells was introduced by high...