A5034150931- Neonatal Respiratory Health Research
- Ion Transport and Channel Regulation
- Ion channel regulation and function
- COVID-19 Clinical Research Studies
- Respiratory Support and Mechanisms
- Inhalation and Respiratory Drug Delivery
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Renal and related cancers
- Congenital Diaphragmatic Hernia Studies
- Extracellular vesicles in disease
- Magnesium in Health and Disease
- SARS-CoV-2 and COVID-19 Research
- Neuroscience and Neuropharmacology Research
- Dermatological and COVID-19 studies
- SARS-CoV-2 detection and testing
- Medical Imaging and Pathology Studies
- Cystic Fibrosis Research Advances
- Immune cells in cancer
- Venous Thromboembolism Diagnosis and Management
- Asthma and respiratory diseases
- Mesenchymal stem cell research
- Diet and metabolism studies
- 3D Printing in Biomedical Research
- Mechanical Circulatory Support Devices
- Epigenetics and DNA Methylation
Chinese Academy of Fishery Sciences
2025
The University of Texas Health Science Center at Tyler
2014-2025
Loyola University Chicago
2023-2025
Affiliated Hospital of Nantong University
2025
Nantong University
2025
Loyola University Medical Center
2024
The University of Texas at Tyler
2019-2022
China Medical University
2018-2022
Xinxiang Medical University
2017-2018
Guangdong Pharmaceutical University
2009
The hypothesis that amiloride-sensitive Na + channels (ENaC) are involved in cell volume regulation was tested. Anisosmotic ND-20 media (ranging from 70 to 450 mosM) were used superfuse Xenopus oocytes expressing αβγ-rat ENaC (αβγ-rENaC). Whole currents reversibly dependent on external osmolarity. Under conditions of swelling (70 or shrinkage (450 mosM), current amplitude decreased and increased, respectively. In contrast, there no change H 2 O-injected the above osmotic insults. Currents...
Beginning from the end of 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic swept all over world and is still afflicting whole global population. Given that vaccine-manufacturing ability limited virus can evolve quickly, vaccination alone may not be able to pandemic, thus developing fast accurate diagnoses effective therapeutics will always unmet needs. Phage display peptide library has been used in screening antigen-specific peptides for invention novel mimic...
Airway and alveolar fluid clearance is mainly governed by vectorial salt movement via apically located rate-limiting Na(+) channels (ENaC) basolateral Na(+)/K(+)-ATPases. ENaC regulated a spectrum of protein kinases, i.e. kinase A (PKA), C (PKC), G (PKG). However, the molecular mechanisms for regulation cGMP/PKG remain to be elucidated. In present study, we studied pharmacological responses native epithelial in human Clara cells alphabetagammadelta ENaCs expressed oocytes cGMP. 8-pCPT-cGMP...
Emerging evidence suggests that Gram-negative bacteria release bacterial outer membrane vesicles (OMVs) and these play an important role in the pathogenesis of infection-mediated inflammatory responses organ damage. Despite fact scattered reports have shown OMVs released from may function via TLR2/4-signaling pathway or induce pyroptosis macrophages, our study reveals a more complex development lung macrophage pro-inflammatory activation. We first confirmed various types similar which prompt...
Abstract Myelodysplastic syndromes (MDS) are a heterogeneous group of pre-leukemic hematopoietic disorders characterized by cytopenia in peripheral blood due to ineffective hematopoiesis and normo- or hypercellularity morphologic dysplasia bone marrow (BM). An inflammatory BM microenvironment programmed cell death stem/progenitor cells (HSPCs) thought be the major causes MDS. Pyroptosis, apoptosis necroptosis (collectively, PANoptosis) observed tissues MDS patients, suggesting an important...
Epithelial sodium channels (ENaC) are essential for (Na + ) transport and maintaining fluid balance, which is vital the removal of fetal at birth homeostasis luminal in lungs. In mice, ENaC composed three subunits (α, β, γ). However, humans, a fourth δ-subunit also expressed. This study investigated physiological role δ-ENaC fetal/neonatal lungs, an area that remains less explored despite its potential significance. We measured expansion mouse E15 lung explants expressing human (SCNN1D-Tg)....
Lung oedema in association with suppressed fibrinolysis is a hallmark of lung injury. Here, we have tested whether plasmin cleaves epithelial sodium channels (ENaC) to resolve fluid.Human lungs and airway acid-instilled mice were used for analysing fluid resolution. In silico prediction, mutagenesis, Xenopus oocytes, immunoblotting, voltage clamp, mass spectrometry, protein docking combined identifying cleavage sites.Plasmin improved resolution both human ex vivo injured mice. Plasmin...
1 The cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel is activated by protein kinase A (PKA) phosphorylation of its R domain and ATP binding at nucleotide-binding domains (NBDs). Here we investigated the functional role a cluster acidic residues in amino terminal tail (N-tail) that also modulate CFTR gating an unknown mechanism. 2 disease-associated mutant lacks one these (D58N CFTR) exhibited lower macroscopic currents Xenopus oocytes faster deactivation...
Abstract Background Chronic tissue injury was shown to induce progressive scarring in fibrotic diseases such as idiopathic pulmonary fibrosis (IPF), while an array of repair/regeneration and stress responses come equilibrium determine the outcome at organ level. In lung, type I alveolar epithelial (ATI) cells constitute barrier, II (ATII) play a pivotal role regenerating injured distal lungs. It had been demonstrated that eukaryotic possess repair machinery can quickly patch damaged plasma...
Abstract Background Acute lung injury is characterized by overwhelmingly elevated PAI-1 in both edema fluid and the circulating system. The role of increased PAI-1, encoded Serpine1 gene, regeneration injured epithelium has not been understood completely. This study aimed to investigate regulation alveolar type 2 epithelial cell (AT2) fate a humanized mouse line carrying diseased mutants ( Tg ). Methods Wild-type (wt) AT2 cells were either cultured as monolayers or 3D alveolospheres....
Studies showed that TRIM72 is essential for repair of alveolar cell membrane disruptions, and exogenous recombinant human protein (rhT72) demonstrated tissue-mending properties in animal models tissue injury. Here we examine the mechanisms rhT72-mediated lung protection vitro test efficacy inhaled rhT72 reducing pathology a mouse model ventilator-induced In injury was induced by glass beads stretching. Ventilator-induced modeled injurious ventilation at 30 ml/kg tidal volume....
Crotonaldehyde is a highly noxious α,β-unsaturated aldehyde in cigarette smoke that causes edematous acute lung injury.To understand how crotonaldehyde impairs function, we examined its effects on human epithelial sodium channels (ENaC), which are major contributors to alveolar fluid clearance.We studied clearance C57 mice and ENaC activity was H441 cells. Expression of α- γ-ENaC measured at protein mRNA levels by western blot real-time PCR, respectively. Intracellular ROS were detected the...
To perform a qualitative and quantitative analysis of catechin quercetin in flavonoids extracted from Rosa roxburghii Tratt.Total were determined using ultraviolet spectrophotometry (UV) at 500 nm. The optimal gradient program started with 15 % methanol was kept within period 0 - 20 min, while 25 33 min. Subsequently, the concentration reduced to held for 10 min until next injection. Mass spectrometry spray voltage 4,000 V, ionization temperature 350 °C, atomizer pressure 35 psi, nitrogen...
Although sodium channels have been a hot multidisciplinary focus for decades and most of nerve system drugs worked on alerting channel function, the trends future directions studies not comprehensive analyzed bibliometrically. Herein, we collected scientific publications research constructed model to evaluate current trend systematically.Publications were selected from Web Science Core Collection (WoSCC) database 2013 2017. Microsoft Excel 2016, Prism 6, CiteSpace V software used analyze...