A5100362566- Metabolomics and Mass Spectrometry Studies
- Advanced biosensing and bioanalysis techniques
- Diet, Metabolism, and Disease
- Diet and metabolism studies
- Neuroscience and Neuropharmacology Research
- Biosensors and Analytical Detection
- Mitochondrial Function and Pathology
- MicroRNA in disease regulation
- Probiotics and Fermented Foods
- Cancer, Hypoxia, and Metabolism
- Analytical chemistry methods development
- Cancer-related molecular mechanisms research
- Gut microbiota and health
- Radioactive element chemistry and processing
- Antibiotics Pharmacokinetics and Efficacy
- Retinal Diseases and Treatments
- CRISPR and Genetic Engineering
- Acute Lymphoblastic Leukemia research
- Liver Disease Diagnosis and Treatment
- Glaucoma and retinal disorders
- Inflammasome and immune disorders
- Tryptophan and brain disorders
- Plant Micronutrient Interactions and Effects
- Sleep and related disorders
- Piperaceae Chemical and Biological Studies
University of Oslo
2022-2025
Akershus University Hospital
2022-2025
Jiangnan University
2019-2025
Kunming Medical University
2025
State Key Laboratory of Food Science and Technology
2019-2025
Nanjing Medical University
2024-2025
The Ohio State University
2021-2025
Hebei Medical University
2023-2024
Second Hospital of Hebei Medical University
2024
Tianjin Medical University
2023-2024
Abstract Background Chimeric antigen receptor (CAR)-T immunotherapy fails to treat solid tumors due in part immunosuppressive microenvironment. Excess lactate produced by tumor glycolysis increases CAR-T immunosuppression. The mechanism of inducing the formation microenvironment remains be further explored. Methods Immunocyte subpopulations and molecular characteristics were analyzed orthotopic xenografts nude mice using flow cytometry assay immunohistochemical staining after oxamate, a...
The development of a sensitive, facile, and cost-effective colorimetric method is great significance for the point-of-care testing viral nucleic acid. Herein, we reported strand displacement amplification assisted CRISPR-Cas12a (SDACC) analysis hepatitis B virus (HBV) DNA was chosen as target to trigger (SDA) generate abundant single-strand (ssDNA) products. ssDNA amplicon hybridized with template activate trans-cleavage activity CRISPR-Cas12a, leading nonspecific cleavage on...
Timely diagnosis is of great benefit to improve the survival rate cancer patients. Body fluid biomarker detection a critical kind noninvasive method for diagnosis. Nevertheless, traditional methods always rely on large-scale instrument and involve sophisticated operation. Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas)-based in vitro can simplify procedures sensitivity specificity, holding promise as next-generation molecular diagnostic...
Abstract The development of single-molecule co-agonists for the glucagon-like peptide-1 (GLP-1) receptor (GLP-1R) and glucose-dependent insulinotropic polypeptide (GIP) (GIPR) is considered a breakthrough in treatment obesity type 2 diabetes. But although GIPR–GLP-1R co-agonism decreases body weight with superior efficacy relative to GLP-1R agonism alone preclinical 1–3 clinical studies 4,5 , role GIP regulating energy metabolism remains enigmatic. Increasing evidence suggests that...
Lacto-N-triose II (LNTII), lacto-N-tetraose (LNT), and lacto-N-neotetraose (LNnT) are key neutral human milk oligosaccharides (HMOs) with significant physiological functions. In cell factory-based synthesis, glycosyltransferases rate-limiting enzymes, enhancing the activity of which is, therefore, crucial for efficient synthesis HMOs. To address this issue, we developed a high-throughput screening method based on UDP-glucose regeneration-coupled colorimetric reaction. As case study, applied...
Chili peppers exhibit antiobesity, anticancer, antidiabetic, and pain- itch-relieving effects on animals humans; these are due to capsaicin, which is the main pungent biologically active component of pepper. Capsiate, a nonpungent capsaicin analogue, similar in terms structure biological activity. In this study, we investigated whether capsiate same hypoglycemic rats with type 1 diabetes (T1D). Experimental were categorized into four groups: control, model, groups. The two treatment groups...
Abstract Growing evidence indicates that there is an emerging link between environmental pollution and cardiac hypertrophy, while the mechanism unclear. The objective of this study was to examine whether phenanthrene (Phe) could cause hypertrophy elucidate molecular mechanisms involved. We found that: 1) Phe exposure increased heart weight cardiomyocyte size rats; 2) led enlarged cell protein synthesis in H9C2 cells; 3) induced important markers such as atrial natriuretic peptide, B-type...
Dysfunction of neuronal nitric oxide synthase contributes to neurotoxicity, which triggers cell death in various neuropathological diseases, including epilepsy. Studies have shown that inhibition activity increases the epilepsy threshold, is, has an anticonvulsant effect. However, exact role and potential mechanism seizures are still unclear. In this study, we performed RNA sequencing, functional enrichment analysis, weighted gene coexpression network analysis hippocampus tremor rats, a rat...
It has been reported that organotin compounds such as triphenyltin or tributyltin (TBT) induce diabetes and insulin resistance. However, histopathological effects of on the Islets Langerhans exocrine pancreas are still unclear. In present study, male KM mice were orally administered with TBT (0.5, 5, 50 μg/kg) once every 3 days. The fasting plasma glucose levels significantly elevated, serum glucagon decreased in animals treated for 60 45 days, number apoptotic cells islets was elevated a...
This study aimed to evaluate the hypoglycemic effect of Zanthoxylum alkylamides and explore potential mechanism in streptozotocin (STZ)-induced diabetic rats. Diabetic rats were orally treated with 3, 6, 9 mg per kg bw daily for 28 days. As alkylamide dose increased, relative weights liver kidney, fasting blood glucose, fructosamine levels significantly decreased. The also increased body weight improved oral glucose tolerance Likewise, muscle glycogen plasma insulin. These substances further...
Supramolecular hydrogels self-assembled from naturally occurring small molecules (e.g., glycyrrhizic acid, GA) are promising materials for controlled bioactive delivery due to their facile fabrication processes, excellent biocompatibility, and versatile stimuli-responsive behaviors. However, most of these natural suffer poor mechanical strength processability practical applications. In this work, through adopting a multicomponent gel approach, we developed novel mechanically robust GA-based...
The mucin-degrading gut commensal Akkermansia muciniphila (A. muciniphila) negatively correlates with various diseases, including metabolic disorders, neurodegenerative and cancers, through interacting host receptors by diverse molecules. Still, their exact capability within the nutrient-rich environment (such as in human gut) is not fully characterized. Therefore, present study, we investigated comprehensive metabolome lipidome of A. after supplementation four major microbial nutrients:...
The pathogenesis of pancreatic ductal adenocarcinoma (PDAC) remains poorly understood. S100 calcium-binding protein A6 (S100A6) has been associated with PDAC; however, the effect S100A6 on PDAC migration and invasion not yet explored. In this study, Panc-1 cells were transfected a plasmid to induce overexpression S100A6, β-catenin was knocked down using specific short hairpin RNA (shRNA). wound-healing Transwell assays demonstrated that promoted cell invasion. Furthermore, shRNA inhibited...
Quercetin (Que) has been proven to enhance the chemosensitivity of multiple cancers, including colon cancer (CC). However, whether combination Que and 5-fluorouracil (5-FU) a synergistic effect on drug-resistant CC cells not previously reported. The (5 10 μg/mL) cell vitality apoptosis was examined using counting kit-8 (CCK-8) flow cytometry. After were treated with 5-FU (10, 40 μg/mL), (10 μM, μM), or in Que, proliferation, apoptosis, oxidative stress-related factors, reactive oxygen...
Abstract Disseminated intravascular coagulation (DIC) is considered to be the most common and lethal complication of sepsis. NLR-family pyrin domain-containing-3 (NLRP3) inflammasome plays an important role in host defense against microbial pathogens, its deregulation may cause cascade should strictly managed. Here, we identified deubiquitinase YOD1, which played a vital regulating NLRP3 inflammasome-dependent manner sepsis induced by methicillin-resistant Staphylococcus aureus (MRSA). YOD1...
Na+/H+ antiporters (NHXs) are integral membrane transporters that catalyze the electroneutral exchange of K+ or Na+ for H+ and implicated in cell expansion, development, pH ion homeostasis salt tolerance. Arabidopsis contains four vacuolar NHX isoforms (NHX1–NHX4), but only functional roles NHX1 NHX2 have been assessed thus far. Colocalization studies indicated NHX3 NHX4 colocalize to tonoplast. To investigate role all isoforms, a quadruple knockout nhx1nhx2nhx3nhx4, lacking NHXs, was...