A5065612462- Bacteriophages and microbial interactions
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Genetics and Biotechnology
- Glycosylation and Glycoproteins Research
- Supramolecular Self-Assembly in Materials
- RNA and protein synthesis mechanisms
- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Molecular Sensors and Ion Detection
- Polydiacetylene-based materials and applications
- Luminescence and Fluorescent Materials
- Nanoplatforms for cancer theranostics
- Antimicrobial Peptides and Activities
University of Kent
2021-2025
We describe an innovative system that exports diverse recombinant proteins in membrane-bound vesicles from E. coli. These compartmentalize within a micro-environment enables production of otherwise challenging insoluble, toxic, or disulfide-bond containing bacteria. The release vesicle-packaged supports isolation the culture and allows long-term storage active protein. This technology results high yields vesicle-packaged, functional for efficient downstream processing wide range applications...
The rise of antimicrobial resistant (AMR) infection represents a growing threat to the global population and economic health. majority innovations are developed against planktonic microorganisms, however those same microorganisms contained within biofilm can become over 1000 times more (including antibiotic) agents. Supramolecular self-associating amphiphiles (SSAs) class amphiphilic salts related compounds that have shown potential for development into antibiofilm Within scope this work we...
Supramolecular self-associating amphiphiles form a variety of self-associated structures demonstrating antimicrobial activity, limited toxicity and number structure activity relationships.
This innovative system, using a short peptide tag, that exports multiple recombinant proteins in membrane bound vesicles from E. coli, provides an effective solution to range of problems associated with bacterial protein expression. These compartmentalise within micro-environment facilitates the production otherwise challenging, toxic, insoluble, or disulfide-bond containing bacteria. Protein yield is increased considerably when compared typical expression absence vesicle-nucleating tag. The...
Maintaining membrane integrity is of paramount importance to the survival bacteria as site multiple crucial cellular processes including energy generation, nutrient uptake and antimicrobial efflux. The DedA family integral proteins are widespread in associated with maintaining membrane. In addition, have been linked resistance classes antimicrobials various microorganisms. Therefore, attractive targets for development new antibiotics. Despite members playing a key physiological role many...
Herein, we present the synthesis of two novel adamantane appended supramolecular self-associating amphiphiles. The antimicrobial efficacy these compounds is determined against both clinically relevant Gram-positive methicillin-resistant Staphylococcus aureus and Gram-negative Escherichia coli. We also explore self-associative properties amphiphiles in polar organic DMSO-d6 0.5% H2O (D2O)/EtOH 19:1 solutions, confirming aggregate stability through determination zeta potential values, size a...
Abstract We have developed an innovative system that exports diverse recombinant proteins in membrane bound vesicles from E. coli 1 . These compartmentalise within a micro-environment enables production of otherwise challenging insoluble, toxic, or disulphide-bond containing bacteria. The release vesicle-packaged supports isolation the culture and allows long-term storage active protein. This technology results high yields vesicle-packaged, functional for efficient downstream processing wide...
Summary We describe a novel system that exports diverse recombinant proteins in extracellular vesicles from E. coli . The not only compartmentalise toxic, insoluble and disulphide bond containing soluble functional form (e.g. DNaseI, nanobodies IgG-fusions), but the continued release of inducible vesicle packaged into media supports continuous isolation protein active culture within micro-environment allowing stable long-term storage. This technology results unprecedented yields for...
Abstract Maintaining membrane integrity is of paramount importance to the survival bacteria as site multiple crucial cellular processes including energy generation, nutrient uptake, and antimicrobial efflux. The DedA family integral proteins are widespread in associated with maintaining membrane. In addition, have been linked resistance classes antimicrobials various microorganisms. Therefore, attractive targets for development new antibiotics. Despite members playing a key physiological...