A5091489560- Cancer, Lipids, and Metabolism
- Pharmacogenetics and Drug Metabolism
- Eicosanoids and Hypertension Pharmacology
- Colorectal Cancer Treatments and Studies
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Metabolomics and Mass Spectrometry Studies
- Protein Degradation and Inhibitors
- Circadian rhythm and melatonin
- Light effects on plants
- Autophagy in Disease and Therapy
- Photoreceptor and optogenetics research
- Peptidase Inhibition and Analysis
- Neurobiology and Insect Physiology Research
- Inflammatory mediators and NSAID effects
University of Massachusetts Amherst
2019-2020
Abstract Colon cancer is the third most common and second leading cause of cancer-related death in United States, emphasizing need for discovery new cellular targets. Using a metabolomics approach, we report here that epoxygenated fatty acids (EpFA), which are eicosanoid metabolites produced by cytochrome P450 (CYP) monooxygenases, were increased both plasma colon azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced mice. CYP monooxygenases overexpressed tumor tissues cells. Pharmacologic...
Circadian rhythms are essential for controlling the cell cycle, cellular proliferation, and apoptosis, hence tightly linked to fate. Several recent studies have used small molecules affect circadian oscillations; however, their concomitant effects were not assessed, they been compared under similar experimental conditions. In this work, we use five molecules, grouped into direct versus indirect effectors of clock, modulate periods in a human osteosarcoma line (U2OS) determine influences on...
ABSTRACT Circadian rhythms are essential for controlling the cell cycle, cellular proliferation, and apoptosis, hence, tightly linked to fate. Disruption of circadian has been shown trigger various pathological developments, including cancer. Several recent studies have used a variety small molecules affect oscillations, however, their concomitant effects were not assessed. Here, we use five molecules, grouped into direct versus indirect effectors clock, modulate periods in human...
SUMMARY The linkage, length, and architecture of ubiquitin (Ub) chains are all important variables in providing tight control over many biological paradigms. There clear roles for branched architectures regulating proteasome-mediated degradation, however the proteins that selectively recognize process these atypical unknown. Here, using synthetic enzyme-derived along with intact mass spectrometry, we report UCH37/UCHL5, a proteasome-associated deubiquitinase, exclusively cleaves K48 chains....
<div>Abstract<p>Colon cancer is the third most common and second leading cause of cancer-related death in United States, emphasizing need for discovery new cellular targets. Using a metabolomics approach, we report here that epoxygenated fatty acids (EpFA), which are eicosanoid metabolites produced by cytochrome P450 (CYP) monooxygenases, were increased both plasma colon azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced mice. CYP monooxygenases overexpressed tumor tissues...
<p>SF1: CYP monooxygenase expression in human colon cancer cells; SF2: gene of Cyp2e1 the liver Cyp2c+/+, Cyp2c+/-, and Cyp2c-/- mice; SF3: inflammation SF4: survival curve AOM/DSS-stimulated SF5: pharmacological inhibition monooxygenases suppresses AOM/DSS-induced tumorigenesis SF6: effects 12,13-EpOME on MC38 tumor growth C57BL/6 SF7: mRNA levels control subjects patients</p>
<p>Supplementary Materials and Methods</p>
<p>Supplementary Materials and Methods</p>
<p>SF1: CYP monooxygenase expression in human colon cancer cells; SF2: gene of Cyp2e1 the liver Cyp2c+/+, Cyp2c+/-, and Cyp2c-/- mice; SF3: inflammation SF4: survival curve AOM/DSS-stimulated SF5: pharmacological inhibition monooxygenases suppresses AOM/DSS-induced tumorigenesis SF6: effects 12,13-EpOME on MC38 tumor growth C57BL/6 SF7: mRNA levels control subjects patients</p>
<div>Abstract<p>Colon cancer is the third most common and second leading cause of cancer-related death in United States, emphasizing need for discovery new cellular targets. Using a metabolomics approach, we report here that epoxygenated fatty acids (EpFA), which are eicosanoid metabolites produced by cytochrome P450 (CYP) monooxygenases, were increased both plasma colon azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced mice. CYP monooxygenases overexpressed tumor tissues...