A5074640890- Insect symbiosis and bacterial influences
- Parasitic Diseases Research and Treatment
- Genomics and Phylogenetic Studies
- Nematode management and characterization studies
- Parasite Biology and Host Interactions
- Coral and Marine Ecosystems Studies
- Marine Sponges and Natural Products
- Aquaculture disease management and microbiota
- Coastal wetland ecosystem dynamics
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Cell Image Analysis Techniques
- Machine Learning in Bioinformatics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Phytoplasmas and Hemiptera pathogens
- Studies on Chitinases and Chitosanases
- Environmental DNA in Biodiversity Studies
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Carbohydrate Chemistry and Synthesis
- Marine and coastal plant biology
- Plant Parasitism and Resistance
- Genetic diversity and population structure
- Advanced Fluorescence Microscopy Techniques
New England Biolabs (United States)
2016-2021
Protein Express (United States)
2020-2021
Northeastern University
2013-2016
Coral diseases are among the most serious threats to coral reefs worldwide, yet remain poorly understood. How host responds pathogen infection is an area where very little known. Here we used next-generation RNA-sequencing (RNA-seq) produce a transcriptome-wide profile of immune response Staghorn Acropora cervicornis White Band Disease (WBD) by comparing infected versus healthy (asymptomatic) tissues. The transcriptome A. was assembled de novo from A-tail selected Illumina mRNA-seq data...
Relationships between the five extant orders of centipedes have been considered solved based on morphology. Phylogenies samples up to a few dozen genes largely congruent with morphological tree apart from an alternative placement one order, relictual Craterostigmomorpha, consisting two species in Tasmania and New Zealand. To address this incongruence, novel transcriptomic data were generated sample all also used as test case for studying gene-tree incongruence. Maximum likelihood Bayesian...
Coral reefs are declining worldwide due to multiple factors including rising sea surface temperature, ocean acidification, and disease outbreaks. Over the last 30 years, White Band Disease (WBD) alone has killed up 95% of Caribbean`s dominant shallow-water corals—the staghorn coral Acropora cervicornis elkhorn A. palmata. Both corals now listed on US Endangered Species Act, while their recovery been slow, recent transmission surveys indicate that more than 5% resistant. Here we compared...
Abstract Sex determination mechanisms often differ even between related species yet the evolution of sex chromosomes remains poorly understood in all but a few model organisms. Some nematodes such as Caenorhabditis elegans have an XO system while others, filarial parasite Brugia malayi , XY mechanism. We present complete B. genome assembly and define Nigon elements shared with C. which we then map to genomes other more distantly nematodes. find remarkable plasticity chromosome several...
Abstract Enrichment methodologies enable the analysis of minor members in multi-species transcriptomic data. We compared standard enrichment bacterial and eukaryotic mRNA to a targeted using an Agilent SureSelect (AgSS) capture for Brugia malayi , Aspergillus fumigatus Wolbachia endosymbiont B. ( w Bm). Without introducing significant systematic bias, AgSS quantitatively enriched samples, resulting more reads mapping target organism. The AgSS-enriched libraries consistently had positive...
Capturing microbial growth on a macroscopic scale is of great importance to further our understanding life. However, methods for imaging life millimeters centimeters are often limited by designs that have poor environmental control, resulting in dehydration the agar plate within just few days. Here, we created MOCHA (microbial chamber), simple but effective chamber allows users study extended periods (weeks) stable environment. Agar hydration maintained with double-decker design, which two...
Lymphatic filariasis affects ∼120 million people and can result in elephantiasis hydrocele. Here, we report the nearly complete genome sequence of best-studied causative agent lymphatic filariasis, Brugia malayi . The assembly contains four autosomes, an X chromosome, only eight gaps but lacks a contiguous for known Y chromosome.
Here, we present a comprehensive transcriptomics data set of Brugia malayi , its Wolbachia endosymbiont w Bm, and vector host. This study samples from 16 stages across the entire B. life cycle, including stage 1 through 4 larvae, adult males females, embryos, immature microfilariae, mature microfilariae.
To better understand the transcriptomic interplay of organisms associated with lymphatic filariasis, we conducted multispecies transcriptome sequencing (RNA-Seq) on filarial nematode Brugia malayi, its Wolbachia endosymbiont wBm, and laboratory vector Aedes aegypti across entire B. malayi life cycle. In transcription noncoding 6S RNA suggests that it may be a regulator bacterial cell growth, as transcript levels correlate replication rates. For A. aegypti, transcriptional response reflects...
Brugia malayi is a human filarial nematode responsible for elephantiasis, debilitating condition that part of broader spectrum diseases called filariasis, including lymphatic filariasis and river blindness. Almost all species infecting humans live in mutualism with Wolbachia endosymbionts, present somatic hypodermal tissues but also the female germline which ensures their vertical transmission to progeny. These α-proteobacteria potentially provision host essential metabolites protect...
The filarial nematode Brugia malayi is one of the causative agents lymphatic filariasis, a neglected tropical disease that affects 120 million people worldwide. limited effectiveness available anthelmintics and absence vaccine have prompted extensive research on interaction between its obligate bacterial endosymbiont, Wolbachia. Recent studies suggest Wolbachia able to manipulate host immunity but relatively little known about immune system nematodes. Therefore, elucidation mechanisms...
Brugia pahangi is a zoonotic parasite that closely related to human-infecting filarial nematodes. Here, we report the nearly complete genome of pahangi, including assemblies four autosomes and an X chromosome, with only seven gaps. The Y chromosome still not completely assembled.
Lymphatic filariasis is a devastating disease caused by filarial nematode roundworms, which contain obligate
The sequence diversity of natural and laboratory populations Brugia pahangi malayi was assessed with Illumina resequencing followed by mapping in order to identify single nucleotide variants insertions/deletions. In populations, there is a lack on chromosome X relative the autosomes (πX/πA = 0.2), which lower than expected 0.75). A reduction also observed other filarial nematodes neo-X fusions genera Onchocerca Wuchereria, but not those without Loa Dirofilaria. species fusions, abnormally...
Abstract Enrichment methodologies enable analysis of minor members in multi-species transcriptomic analyses. We compared standard enrichment bacterial and eukaryotic mRNA to targeted with Agilent SureSelect (AgSS) capture for Brugia malayi , Aspergillus fumigatus the Wolbachia endosymbiont B. (wBm). Without introducing significant systematic bias, AgSS quantitatively enriched samples, resulting more reads mapping target organism. The AgSS-enriched libraries consistently had a positive linear...