A5070351639- Parasitic Diseases Research and Treatment
- Insect symbiosis and bacterial influences
- Parasite Biology and Host Interactions
- Nematode management and characterization studies
- Genomics and Phylogenetic Studies
- Mosquito-borne diseases and control
- Insects and Parasite Interactions
- Bird parasitology and diseases
- RNA modifications and cancer
- Animal Ecology and Behavior Studies
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Rabies epidemiology and control
- Toxoplasma gondii Research Studies
- Paleopathology and ancient diseases
- Intraperitoneal and Appendiceal Malignancies
- Quantum Dots Synthesis And Properties
- Parasites and Host Interactions
- Animal health and immunology
- Vector-borne infectious diseases
- Mollusks and Parasites Studies
- Metastasis and carcinoma case studies
- Antimicrobial Peptides and Activities
- Plant Parasitism and Resistance
- Parasitic Infections and Diagnostics
University of Wisconsin–Oshkosh
2011-2024
Oshkosh (United States)
2023
Washington University in St. Louis
1998-2002
In a ‘blind’ trial on 50 male asymptomatic microfilaraemic subjects with Wuchereria bancrofti infection, the safety, tolerability and filaricidal efficacy of single dose albendazole (alb) 600 mg alone or in combination ivermectin (iver) 400 μg/kg diethylcarbamazine citrate (DEC) 6 mg/kg was compared DEC iver over period 15 months after treatment. All but one subject, 67 microfilariae (mf)/mL, had pre-treatment counts > 100 mf/mL. 4 treatments significantly reduced mf counts, alb/iver most...
Background Developing intervention strategies for the control of parasitic nematodes continues to be a significant challenge. Genomic and post-genomic approaches play an increasingly important role providing fundamental molecular information about these parasites, thus enhancing basic as well translational research. Here we report comprehensive genome-wide survey developmental transcriptome human filarial parasite Brugia malayi. Methodology/Principal Findings Using deep sequencing, profiled...
While bacterial symbionts influence a variety of host cellular responses throughout development, there are no documented instances in which early embryogenesis. Here we demonstrate that Wolbachia, an obligate endosymbiont the parasitic filarial nematodes, is required for proper anterior-posterior polarity establishment nematode B. malayi. Characterization pre- and post-fertilization events malayi reveals that, unlike C. elegans, centrosomes maternally derived produce cortical-based...
Abstract Sex determination mechanisms often differ even between related species yet the evolution of sex chromosomes remains poorly understood in all but a few model organisms. Some nematodes such as Caenorhabditis elegans have an XO system while others, filarial parasite Brugia malayi , XY mechanism. We present complete B. genome assembly and define Nigon elements shared with C. which we then map to genomes other more distantly nematodes. find remarkable plasticity chromosome several...
Dirofilaria immitis, or canine heartworm, is a filarial nematode parasite that infects dogs and other mammals worldwide. Current disease control relies on regular administration of anthelmintic preventives, however, relatively poor compliance evidence developing drug resistance could warrant alternative measures against D. immitis related human infections be taken. As with many nematodes, contains Wolbachia, an obligate bacterial endosymbiont thought to involved in providing certain critical...
Filarial nematodes cause debilitating human diseases. While treatable, recent evidence suggests drug resistance is developing, necessitating the development of novel targets and new treatment options. Although transcriptomic proteomic studies around nematode life cycle have greatly enhanced our knowledge, whole organism approaches not provided spatial resolution gene expression, which can be gained by examining individual tissues. Generally, due to their small size, tissue dissection...
Dirofilaria ursi is a filarial nematode of American black bears (Ursus americanus Pallas, 1780) that vectored by flies (Simuliidae) in many parts the United States. In northwestern Wisconsin, prevalence microfilaremic during fall hunting season was 21% (n = 47). Unsheathed blood microfilariae from Wisconsin possess characters consistent with original description D. ursi, as do adult worms observed histologically and grossly. Immunohistochemistry used to identify Wolbachia endosymbiont...
Mosquitoes in the Culex pipiens complex thrive temperate and tropical regions worldwide, serve as efficient vectors of Bancroftian lymphatic filariasis (LF) caused by Wuchereria bancrofti Asia, Africa, West Indies, South America, Micronesia. However, members this mosquito do not act natural for Brugian LF Brugia malayi, or cat parasite B. pahangi, despite their presence Asia where these parasites are endemic. Previous work with Iowa strain demonstrates that it is equally susceptible to W....
Abstract Enrichment methodologies enable the analysis of minor members in multi-species transcriptomic data. We compared standard enrichment bacterial and eukaryotic mRNA to a targeted using an Agilent SureSelect (AgSS) capture for Brugia malayi , Aspergillus fumigatus Wolbachia endosymbiont B. ( w Bm). Without introducing significant systematic bias, AgSS quantitatively enriched samples, resulting more reads mapping target organism. The AgSS-enriched libraries consistently had positive...
In the present study, we describe intraperitoneal development of FR3 strain Brugia malayi in Mongolian jirds (Meriones unguiculatus). The third molt for male worms occurred between 4 and 7 days postinfection (dpi) 8 dpi females. fourth final 21 29 males 25 34 females, considerably earlier than times reported subcutaneous infection models using cats jirds. timing coincided largely with reports pahangi infections jirds, but later lasted longer those B. models. Spermatogenesis by at least 50...
Lymphatic filariasis affects ∼120 million people and can result in elephantiasis hydrocele. Here, we report the nearly complete genome sequence of best-studied causative agent lymphatic filariasis, Brugia malayi The assembly contains four autosomes, an X chromosome, only eight gaps but lacks a contiguous for known Y chromosome.
Here, we present a comprehensive transcriptomics data set of Brugia malayi , its Wolbachia endosymbiont w Bm, and vector host. This study samples from 16 stages across the entire B. life cycle, including stage 1 through 4 larvae, adult males females, embryos, immature microfilariae, mature microfilariae.
To better understand the transcriptomic interplay of organisms associated with lymphatic filariasis, we conducted multispecies transcriptome sequencing (RNA-Seq) on filarial nematode Brugia malayi, its Wolbachia endosymbiont wBm, and laboratory vector Aedes aegypti across entire B. malayi life cycle. In transcription noncoding 6S RNA suggests that it may be a regulator bacterial cell growth, as transcript levels correlate replication rates. For A. aegypti, transcriptional response reflects...
Accumulation of DNA sequence data and its use in systematics the family Argasidae reveals new incongruencies between genera subgenera, since several groups defined by classical taxonomy appear to be paraphyletic, which is case subgenus Pavlovskyella. In order identify morphological characters unique one monophyletic groupings within Pavlovskyella improve system, we describe all active stages Ornithodoros (Pavlovskyella) tartakovskyi, a species with an incomplete original description. Larvae,...
Dargestellt wurden drei Falle von intraspinalen Tumoren bei Kindern (Meningeom, Spongioblastom, Sympathikoblastom). Man unterstrich die Diagnoseschwierigkeiten der im fruhen Kindesalter sowie Wichtigkeit Myelographie zur genauen Lokalisierung Tumoren. Die Bedeutung nachfolgenden Kontrolle Kontrastsaule wurde hervorgehoben. Three cases of intraspinal tumours in childhood are described (meningeoma, spongioblastoma, sympathicoblastoma). The diagnostic difficulties posed by early emphasised....
Brugia pahangi is a zoonotic parasite that closely related to human-infecting filarial nematodes. Here, we report the nearly complete genome of pahangi, including assemblies four autosomes and an X chromosome, with only seven gaps. The Y chromosome still not completely assembled.