A5057401220- Drug Solubulity and Delivery Systems
- Crystallization and Solubility Studies
- Analytical Chemistry and Chromatography
- Surfactants and Colloidal Systems
- Advanced Drug Delivery Systems
- Crystallography and molecular interactions
- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Analytical Methods in Pharmaceuticals
- Proteins in Food Systems
- Advanced NMR Techniques and Applications
- Microencapsulation and Drying Processes
- Protein purification and stability
- Microfluidic and Capillary Electrophoresis Applications
- Pharmacological Effects of Natural Compounds
- biodegradable polymer synthesis and properties
- Advanced Polymer Synthesis and Characterization
- NMR spectroscopy and applications
- Computational Drug Discovery Methods
- Material Dynamics and Properties
- Protein Interaction Studies and Fluorescence Analysis
- Supramolecular Chemistry and Complexes
- Drug Transport and Resistance Mechanisms
- Pickering emulsions and particle stabilization
Chiba University
2016-2025
Purdue University West Lafayette
2020-2021
Kumamoto University
2018
Japan Society for the Promotion of Science
2014
Abstract RNA‐based therapeutics is a promising approach for curing intractable diseases by manipulating various cellular functions. For eliciting RNA (i.e., mRNA and siRNA) functions successfully, the in extracellular space must be protected it delivered to cytoplasm. In this study, development of self‐degradable lipid‐like material that accelerate collapse lipid nanoparticles (LNPs) release into cytoplasm reported. The self‐degradability based on unique reaction “Hydrolysis accelerated...
We examined the inhibitory effect of hydroxypropyl methylcellulose acetate succinate (HPMC-AS) on drug recrystallization from a supersaturated solution using carbamazepine (CBZ) and phenytoin (PHT) as model drugs. HPMC-AS HF grade (HF) inhibited CBZ more strongly than that by LF (LF). 1D-1H NMR measurements showed molecular mobility was clearly suppressed in compared to solution. Interaction between directly detected nuclear Overhauser spectroscopy (NOESY). The cross-peak intensity obtained...
The polymer used in an amorphous solid dispersion (ASD) formulation plays a critical role dosage form performance. Herein, drug-polymer interactions aqueous solution were evaluated order to better understand the stability of colloidal drug-rich phase generated when solubility is exceeded. (Sa,IBP) ibuprofen (IBP) decreased hypromellose (HPMC) or polyvinylpyrrolidone/vinyl acetate (PVP-VA) present solution. Solution nuclear magnetic resonance (NMR) spectroscopy revealed that large amount HPMC...
Based on the clinical success of an in vitro transcribed mRNA (IVT-mRNA) that is encapsulated lipid nanoparticles (mRNA-LNPs), there a growing demand by researchers to test whether their own biological findings might be applicable for use mRNA-based therapeutics. However, equipment and/or know-how required manufacturing such often inaccessible. To encourage more innovation therapeutics, simple method preparing mRNA-LNPs prerequisite. In this study, we report encapsulating IVT-mRNA into LNPs...
In this study, we investigated the mechanism of curcumin (CUR) release from poly(lactic-co-glycolic acid) (PLGA) and poly(lactic (PLA) nanoparticles (NPs) by evaluating temperature-dependent CUR release. NPs were prepared nanoprecipitation method using various PLGA/PLA polymers with different lactic:glycolic ratios (L:G ratios) molecular weights. Increasing polymer weight resulted in a decrease particle size NPs. The wet glass transition temperature (Tg) was lower than intrinsic Tg, which...
A solid dispersion (SPD) of carbamazepine (CBZ) with hydroxypropyl methylcellulose acetate succinate (HPMC-AS) was prepared by the spray drying method. The apparent solubility (37 °C, pH 7.4) CBZ observed SPD over 3 times higher than unprocessed CBZ. supersaturated solution stable for 7 days. concentration in aqueous medium also achieved mixing Poloxamer 407 (P407), a solubilizing agent. From permeation studies using Caco-2 monolayers and dialysis membranes, we improved across membrane...
We investigated the phase separation behavior and maintenance mechanism of supersaturated state poorly water-soluble nifedipine (NIF) in hypromellose (HPMC) derivative solutions. Highly NIF formed NIF-rich nanodroplets through from aqueous solution containing HPMC derivative. Dissolvable concentration bulk water was limited by solution. derivatives stabilized maintained supersaturation with phase-separated for several hours. The size different depending on dissolved solution, although...
In the present study, molecular state of drug-rich amorphous nanodroplets was evaluated using NMR techniques to reveal mechanism underlying crystallization inhibition by a polymer. Ibuprofen (IBP) with low glass transition temperature used for direct characterization nanodroplets. Highly supersaturated IBP formed IBP-rich through phase separation from aqueous solution. Increasing concentration hypromellose (HPMC) in solution contributed and maintenance supersaturation at solubility. Solution...
In this study, the time-dependent evolution of amorphous probucol nanoparticles was characterized by cryogenic transmission electron microscopy (cryo-TEM) and atomic force (AFM). The were formed dispersing ternary solid dispersions in water. Spray drying cogrinding used to prepare a spray-dried sample (SPD) two ground-mixture samples (GM(I) GM(II)) (PBC) form I II/hypromellose/sodium dodecyl sulfate dispersions. amorphization PBC SPDs GMs confirmed using powder X-ray diffraction (PXRD)...
The polymer used in an amorphous solid dispersion (ASD) formulation impacts the maximum achievable drug supersaturation. Herein, effect of dissolved on concentration aqueous phase when a drug-rich was generated by liquid–liquid separation (LLPS) investigated for different polymers at various concentrations and polymer. Solution nuclear magnetic resonance (NMR) spectroscopy revealed that polyvinylpyrrolidone (PVP), polyvinylpyrrolidone/vinyl acetate (PVP-VA), hypromellose (HPMC) distributed...
Coamorphous formulation is a useful approach for enhancing the solubility of poorly water-soluble drugs via intermolecular interactions. In this study, hydrogen-bonding-based coamorphous system was developed to improve drug solubility, but it barely changed apparent permeability (Papp) drug. This study aimed design novel salt using ionic interactions and absorption. Telmisartan (TMS), with an acidic group, used form basic amlodipine (AML). Evaluation physicochemical properties confirmed...
RNA vaccines are applicable to the treatment of various infectious diseases via inducement robust immune responses against target antigens by expressing antigen proteins in human body. The delivery messenger lipid nanoparticles (LNPs) has become a versatile drug system used administration vaccines. LNPs widely considered possess adjuvant activity that induces strong response. However, properties contribute their continue require clarification. To characterize relationships between...
Drug-rich droplets formed through liquid–liquid phase separation (LLPS) have the potential to enhance oral absorption of drugs. This can be attributed diffusion these into unstirred water layer (UWL) gastrointestinal tract and their reservoir effects on maintaining drug supersaturation. However, a quantitative understanding effect drug-rich intestinal is still lacking. In this study, enhancement formation was quantitatively evaluated mechanistic basis. To obtain fenofibrate (FFB)-rich...
Supersaturatable self-microemulsifying drug delivery system (S-SMEDDS) has recently been utilized to enhance the oral absorption of poorly water-soluble drugs. S-SMEDDS forms drug-incorporated microemulsions (MEs) during aqueous dispersion with formation supersaturation in bulk water phase. However, liquid–liquid phase separation (LLPS) behavior supersaturated drugs within MEs not well studied. This study investigated impact components on LLPS and achievable level MEs. Fenofibrate...
Lipid nanoparticles with encapsulated mRNA (mRNA-LNPs) have become key modalities for personalized medicines and RNA vaccines. Once the platform technology is established, mRNA-LNPs could be applicable to a variety of protein-based therapeutic strategies. A post-encapsulation method, in which solution incubated preformed mRNA-free LNPs prepare mRNA-LNPs, would accelerate development RNA-based therapeutics since even nonexperts manufacture mRNA-LNPs. In this study, we describe that into...