A5016894341- Metabolomics and Mass Spectrometry Studies
- Forensic Entomology and Diptera Studies
- Advanced NMR Techniques and Applications
- Atomic and Subatomic Physics Research
- Metabolism and Genetic Disorders
- Meat and Animal Product Quality
- Thermal Regulation in Medicine
- Antibiotics Pharmacokinetics and Efficacy
- Quantum, superfluid, helium dynamics
- Cardiac Arrest and Resuscitation
- Hemoglobin structure and function
- Neonatal Respiratory Health Research
- Neonatal and fetal brain pathology
- Isotope Analysis in Ecology
- Traditional Chinese Medicine Studies
- Glaucoma and retinal disorders
- Cytomegalovirus and herpesvirus research
- Forensic Anthropology and Bioarchaeology Studies
- Advanced Chemical Sensor Technologies
- Antimicrobial Resistance in Staphylococcus
- Acute Kidney Injury Research
- Diet and metabolism studies
- Neuroscience of respiration and sleep
- NMR spectroscopy and applications
- Appendicitis Diagnosis and Management
University of Cagliari
2015-2025
University of Turin
2023
Istituto Nazionale di Fisica Nucleare, Sezione di Cagliari
2003-2020
Université Libre de Bruxelles
2002-2010
University of Milan
2003
The combined use of multiple omics allows to study complex interrelated biological processes in their entirety. We applied a combination metabolomics, lipidomics and proteomics human bones investigate potential estimate time elapsed since death (i.e., the postmortem interval [PMI]). This ‘ForensOMICS’ approach has improve accuracy precision PMI estimation skeletonized remains, thereby helping forensic investigators establish timeline events surrounding death. Anterior midshaft tibial bone...
Abstract Introduction The estimation of post-mortem interval (PMI) remains a major challenge in forensic science. Most the proposed approaches lack reliability required to meet rigorous standards. Objectives We applied 1 H NMR metabolomics estimate PMI on ovine vitreous humour comparing results with actual scientific gold standard, namely potassium concentrations. Methods Vitreous samples were collected time frame ranging from 6 86 h after death. Experiments performed by using and ion...
Heart failure (HF) is characterized by a series of adaptive changes in energy metabolism. The use metabolomics enables the parallel assessment wide range metabolites. In this study, we appraised whether metabolic correlate with HF severity, assessed as an impairment functional contractility, and attempted to interpret role determining systolic dysfunction. A 500 MHz proton nuclear magnetic resonance (1H-NMR)-based analysis was performed on blood samples from three groups individuals: 9...
Vancomycin and piperacillin/tazobactam are reported in clinical studies to increase acute kidney injury (AKI). However, no study has demonstrated synergistic toxicity, only that serum creatinine increases.To clarify the potential for toxicity between vancomycin, vancomycin + treatments by quantifying a translational rat model of AKI using cell studies.(i) Male Sprague-Dawley rats (n = 32) received saline, 150 mg/kg/day intravenously, 1400 intraperitoneally or 3 days. Urinary biomarkers...
Estimation of the post-mortem interval (PMI) remains a matter concern in forensic scenario. Traditional and novel approaches are not yet able to fully address this issue, which relies on complex biological phenomena triggered by death. For purpose, eye compartments may be chosen for experimental studies because they more resistant modifications. Vitreous humour, particular, has been extensively investigated, with potassium concentration ([K+]) being marker that is better correlated PMI...
Due to its peculiar anatomy and physiology, the pericardial fluid is a biological matrix of particular interest in forensic field. Despite this, available literature has mainly focused on post-mortem biochemistry toxicology, while best authors' knowledge metabolomics never been applied. Similarly, estimation time since death or interval based fluids still rarely attempted.
Abstract β ‐Cyclodextrin ( ‐CD) inclusion complexes with carvacrol 1 ), thymol 2 and eugenol 3 ) (components of essential oils vegetable origin) were prepared by the supercritical CO technique, their structural characterization was achieved means H‐NMR in aqueous solution 13 C‐CPMAS NMR solid state. Evidence formation for all examined systems obtained solution, while 2D‐ROESY‐NMR experiments used to investigate geometry inclusion. In addition, dynamics these kHz timescale investigated...
We applied a metabolomic approach to monitor the modifications occurring in goat vitreous humor (VH) metabolite composition at different times (0, 6, 12, 18, and 24 hours) after death. The (1)H-NMR analysis of VH samples was performed for simultaneous determination several metabolites (i.e., profile) representative status times. Spectral data were analyzed by Principal Component Analysis (PCA) Orthogonal Projection Latent Structures (OPLS) regression technique. PCA OPLS suggested that...
The integration of omics sciences, such as genomics, transcriptomics, proteomics, lipidomics, and metabolomics, into forensic practice holds substantial promise for advancing personalized medicine. This short communication aims to elucidate the complexities associated with implementing these advanced methodologies within contexts, particularly in relation legal admissibility under standards established by landmark U.S. Supreme Court case, Daubert v. Merrell Dow Pharmaceuticals. Omics...
Abstract Translating findings from animal models to human applications remains a fundamental challenge across scientific research, with unique implications for post-mortem metabolomics. This study advances this goal by applying 1 H NMR metabolomics aqueous humour, building on previously published sheep model interval estimation. We constructed dataset of 21 humor samples 11 forensic autopsies, intervals (225– 1164 minutes) aligned the model’s time range. Quantification 46 metabolites...
The purpose of this study was to evaluate the feasibility a (1)H-NMR-based metabolomic approach explore signature different topographical areas vitreous humor (VH) in an animal model. Five ocular globes were enucleated from five goats and immediately frozen at -80 °C. Once frozen, three them sectioned, four samples corresponding VH collected: cortical, core, basal, which further divided into superior inferior fraction. An additional two collected that representative whole body. (1)H-NMR...
Perinatal asphyxia is an event affecting around four million newborns worldwide. The 0.5 to 2 per 1000 of full term asphyxiated suffer from hypoxic-ischemic encephalopathy (HIE), which a frequent cause death or severe disability and, as consequence, the most common birth injury claim for obstetrics, gynaecologists, and paediatricians. results compromised gas exchange that leads hypoxemia, hypercapnia, metabolic acidosis. In this work, we applied metabolomics approach investigate profiles...
The diagnosis of pain nature is a troublesome task and wrong attribution often leads to an increase costs avoidable pharmaceutical adverse reactions. An objective specific approach achieve this highly desirable. aim work was investigate urine samples collected from patients suffering different by metabolomics based on (1)H NMR spectroscopy multivariate statistical analysis. We performed prospective study 74 subjects: 37 (12 with nociceptive 25 neuropathic pain), controls not any kind chronic...
A growing body of evidence suggests that the post-mortem interval exerts a strong effect on metabolome, independently biological matrix or cause death. sound and shared approach in standardization is mandatory.
The diagnosis of mechanical asphyxia remains one the most difficult issues in forensic pathology. Asphyxia ultimately results cardiac arrest (CA) and, as there are no specific markers, differential primitive CA and secondary to asphyxiation relies on circumstantial details pathologist experience, lacking objective evidence. Histological examination is currently considered gold standard for post-mortem diagnosis. Here we present comparative histopathology versus those previously obtained by...