A5035729751- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cancer, Hypoxia, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Cancer Cells and Metastasis
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Neuroblastoma Research and Treatments
- Phagocytosis and Immune Regulation
- Radiopharmaceutical Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Cancer Research and Treatments
- Angiogenesis and VEGF in Cancer
- Immune cells in cancer
- Virus-based gene therapy research
- Reproductive System and Pregnancy
- Cancer, Stress, Anesthesia, and Immune Response
- Pregnancy and preeclampsia studies
- Omental and Epiploic Conditions
- Intraperitoneal and Appendiceal Malignancies
- Effects of Radiation Exposure
- Endometriosis Research and Treatment
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
University of Rochester
2013-2025
University of Rochester Medical Center
2013-2025
Czech Academy of Sciences, Institute of Microbiology
2019
Fox Chase Cancer Center
2015
Bipar
2014
University of Pennsylvania
1997
University of California, Riverside
1994-1996
University of California, Berkeley
1977
University of California, San Francisco
1977
University of California, San Diego
1971-1975
Abstract Immunotherapy of cancer is attractive because its potential for specificity and limited side effects. The efficacy this approach may be improved by providing adjuvant signals an inflammatory environment immune cell activation. We evaluated antitumor responses in mice after treatment OVA-expressing B16-F0 tumors with single (15 Gy) or fractionated (5 × 3 doses localized ionizing radiation. Irradiated had cells greater capability to present tumor Ags specific T that secreted IFN-γ...
Abstract Alterations to the tumor microenvironment following localized irradiation may influence effectiveness of subsequent immunotherapy. The objective this study was determine how IFN-γ influences inflammatory response within dynamic environment radiotherapy. B16/OVA melanoma cells were implanted into C57BL/6 (wild-type (WT)) and IFN-γ-deficient (IFN-γ−/−) mice. Seven days after implantation, mice received 15 Gy assessed 7 later. Irradiation up-regulated expression VCAM-1 on vasculature...
Significant numbers of myocytes die by apoptosis during myocardial infarction. The molecular mechanism this process, however, remains largely unexplored. To facilitate a genetic analysis, we have developed model ischemia-induced cardiac myocyte in the mouse. Surgical occlusion left coronary artery results apoptosis, as indicated presence nucleosome ladders and situ DNA strand breaks. Apoptosis occurs mainly myocytes, is shown for first time to be limited hypoxic regions acute Since...
Cancer treatments using ionizing radiation (IR) therapy are thought to act primarily through the induction of tumor cell damage at a molecular level. However, new concept has recently emerged, suggesting that immune system is required for effective IR therapy. Our work here identified interferon gamma (IFN-γ) as an essential cytokine efficacy Local (15 Gy) mice bearing Colon38, colon adenocarcinoma, decreases burden in wild-type animals. Interestingly, had no effect on IFNγKO mice. We...
In species with endometrial decidualization and hemochorial placentation (humans, mice, others), leukocytes localize to early implant sites contribute decidual angiogenesis, spiral arterial remodeling, trophoblast invasion. Relationships between leukocytes, trophoblasts, the vasculature are not fully defined. Early C57BL/6J were analyzed by flow cytometry define leukocyte subsets whole-mount immunohistochemistry visualize relationships vessels, trophoblasts. Ptprc(+) (CD45(+)) cells...
Significance Precise regulation of chemokine signal is critical for directional migration cells. In the study complex cell behavior, it remains difficult to manipulate activity at precise times and places within living animals, not possible different effects on defined types over a range timescales. Furthermore, given can activate multiple receptors vice versa. Here we developed photoactivatable receptor that induce highly specific signals guide toward light stimulation. This work will...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTRequirement for proliferating cell nuclear antigen expression during stages of the Chinese hamster ovary cycleYin Chang Liu, Robert L. Marraccino, Peter C. Keng, A. Bambara, Edith M. Lord, Wen Gang Chou, and Sayeeda B. ZainCite this: Biochemistry 1989, 28, 7, 2967–2974Publication Date (Print):April 4, 1989Publication History Published online1 May 2002Published inissue 4 April...
Abstract A high molecular weight fraction prepared from human seminal plasma by gel filtration chromatography suppresses lymphocyte transformation and DNA synthesis induced mitogens (PHA, Con A, PWM), antigens (Candida albicans, tetanus toxoid), allogenic cells. This same also the stimulated response of mouse lymphocytes to cells various mitogens, including T cell-dependent cell-independent mitogens. The induction, but not expression, cell-mediated cytotoxicity is suppressed. Similar...
Over 80% of pancreatic ductal adenocarcinoma (PDA) patients are diagnosed with non-resectable late-stage disease that lacks effective neoadjuvant therapies. Stereotactic body radiation therapy (SBRT) has shown promise as an emerging approach for treating PDA, and here, we report its combination local interleukin-12 (IL-12) microsphere (MS) immunotherapy results in marked tumor reduction cures multiple preclinical mouse models PDA. Our findings demonstrate increase intratumoral interferon...
Although radiotherapy (RT) is widely used to control tumor growth across many cancer types, there a relatively high incidence of RT failure exhibited by recurrence, therefore clear need exists achieve improved effectiveness RT. The RT-elicited immune response largely impacts the efficacy and includes cells that kill cells, but also immunosuppressive which dampen anti-tumor immunity. Using murine models in syngeneic cell lines (Colon38, Glioma261, Line1) are grown intramuscularly treated with...
Stereotactic body radiotherapy (SBRT) has been increasingly used as adjuvant therapy in pancreatic ductal adenocarcinoma (PDAC), and induces immunogenic cell death, which leads to the release of tumor antigen damage-associated molecular patterns. However, this induction often fails generate sufficient response overcome pre-existing microenvironment (TME) immunosuppression. Toll-like receptor (TLR) 7/8 ligands, such R848, can amplify effect vaccines, with recent evidence showing its antitumor...
Abstract Treatment of mouse erythrocytes with the proteolytic enzyme, bromelain, reveals antigenic determinants not normally exposed on erythrocyte surface. It was found that only NZB mice, a known autoimmune strain, but also several normal strains mice contain cells in small numbers their spleens and larger peritoneal cavities which will form plaques against bromelain-treated MRBC. During vitro culture number anti-BR-MRBC PFC increases slightly spleen cell populations whereas these...
Abstract A number of T cell functions were followed in mice after thymectomy adult life. It was found that cells involved these could be divided roughly into two subpopulations: one whose activity relatively long-lived (longer than 20 weeks) and one, short-lived (on the order a few weeks). In class those helper function primary IgM IgG responses to SRBC vivo vitro. Priming for secondary demonstration or delayed hypersensitivity specific seemed involve both classes, although contribution from...
Background Stereotactic body radiotherapy (SBRT) induces immunogenic cell death, leading to subsequent antitumor immune response that is in part counterbalanced by activation of evasive processes, for example, upregulation programmed death-ligand 1 (PD-L1) and adenosine generating enzyme, CD73. CD73 upregulated pancreatic ductal adenocarcinoma (PDAC) compared with normal tissue high expression PDACs associated increased tumor size, advanced stage, lymph node involvement, metastasis, PD-L1...