A5020814605- Transgenic Plants and Applications
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Microbial Natural Products and Biosynthesis
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Neurobiology and Insect Physiology Research
- Phytochemicals and Antioxidant Activities
- Biochemical and Structural Characterization
- Plant Virus Research Studies
- Insect Resistance and Genetics
- Phytochemical compounds biological activities
- Insect behavior and control techniques
- Plant Stress Responses and Tolerance
- Entomopathogenic Microorganisms in Pest Control
Norwich Research Park
2012-2024
Earlham Institute
2019-2024
John Innes Centre
2012-2017
Promoters serve a critical role in establishing baseline transcriptional capacity through the recruitment of proteins, including transcription factors. Previously, paucity data for cis-regulatory elements plants meant that it was challenging to determine which sequence plant promoter sequences contributed function. In this study, we have identified functional promoters genes and pathogens utilize machinery gene expression. We established quantitative experimental system investigate function,...
Given the potential health benefits of polyphenolic compounds in diet, there is a growing interest generation food crops enriched with health-protective flavonoids. We undertook series metabolite analyses tomatoes ectopically expressing Delila and Rosea1 transcription factor genes from snapdragon (Antirrhinum majus), paying particular attention to changes phenylpropanoids compared controls. These revealed multiple changes, including depletion rutin naringenin chalcone, enhanced levels...
Previous work has demonstrated that plants can be used as production platforms for molecules in health, medicine, and agriculture. Production been exemplified both stable transgenic using transient expression strategies. In particular, species of Nicotiana have engineered to produce a range useful molecules, including insect sex pheromones, which are valued species-specific control agricultural pests. To date, most studies relied on strong constitutive all pathway genes. However, microbes...
Many goals in synthetic biology, including the elucidation and refactoring of biosynthetic pathways engineering regulatory circuits networks, require knowledge protein function. In plants, prevalence large gene families means it can be particularly challenging to link specific functions individual proteins. However, characterization has remained a technical bottleneck, often requiring significant effort optimize expression purification protocols. To leverage ability biofoundries accelerate...
Insect physiology and reproduction depend on several terpenoid compounds, whose biosynthesis is mainly unknown. One enigmatic group of insect monoterpenoids are mealybug sex pheromones, presumably resulting from the irregular coupling activity unidentified isoprenyl diphosphate synthases (IDSs). Here, we performed a comprehensive search for IDS coding sequences pest
ABSTRACT Promoters serve a critical role in establishing baseline transcriptional capacity through the recruitment of proteins, including transcription factors (TFs). Previously, paucity data for cis -regulatory elements plants meant that it was challenging to determine which sequence plant promoter sequences contributed function. In this study, we have identified functional promoters genes and pathogens utilise machinery gene expression. We established quantitative experimental system...
Abstract Many insect species rely on diverse terpenoids for their development and interorganismal interactions. However, little is known about terpenoid biosynthesis in insects. The monoterpenoid sex pheromones of mealybugs scale insects (Coccoidea) are particularly enigmatic, with several producing unique structures presumed to result from the irregular coupling activity unidentified isopentenyl diphosphate synthases (IDSs). Enzymes capable similar transformations have previously only been...
Summary Previous work has demonstrated that plants can be used as production platforms for molecules in health, medicine, and agriculture. Production been exemplified both stable transgenic using transient expression strategies. In particular, species of Nicotiana have engineered to produce a range useful molecules, including insect sex pheromones, which are valued species-specific control agricultural pests. To date, most studies relied on strong constitutive all pathway genes. However,...
Abstract Many goals in synthetic biology, including the elucidation and refactoring of biosynthetic pathways engineering regulatory circuits networks, require knowledge protein function. In plants, prevalence large gene families means it can be particularly challenging to link specific functions individual proteins. However, characterisation has remained a technical bottleneck, often requiring significant effort optimise expression purification protocols. To leverage ability biofoundries...
Many insect species rely on diverse terpenoids for their development and interorganismal interactions. However, little is known about terpenoid biosynthesis in insects. The monoterpenoid sex pheromones of mealybugs scale insects (Coccoidea) are particularly enigmatic, with several producing unique structures presumed to result from the irregular coupling activity unidentified isopentenyl diphosphate synthases (IDSs). Enzymes capable similar transformations have previously only been described...
Synthetic biology applies the principles of engineering to biology, helping us design, redesign and build novel biological systems. First applied microorganisms it has enabled industrialscale production beneficial, bio-based products such as pharmaceuticals, flavourings textiles. It is now advancing multicellular organisms. In plants, field still in its infancy, yet potential for plants light-powered, sustainable biomanufacturing platforms tremendous.