A5083017421- interferon and immune responses
- RNA regulation and disease
- RNA Research and Splicing
- Light effects on plants
- Circadian rhythm and melatonin
- 3D Printing in Biomedical Research
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Genetics, Aging, and Longevity in Model Organisms
- Viral gastroenteritis research and epidemiology
- Cell Adhesion Molecules Research
- Pluripotent Stem Cells Research
- Influenza Virus Research Studies
- Viral Infections and Immunology Research
- RNA Interference and Gene Delivery
- Neurobiology and Insect Physiology Research
Princeton University
2018-2022
Rockefeller University
2017
University of St Andrews
2015
Nocturnin (NOCT) is a rhythmically expressed protein that regulates metabolism under the control of circadian clock. It has been proposed NOCT deadenylates and metabolic enzyme mRNAs. However, in contrast to other deadenylases, purified lacks deadenylase activity. To identify substrate NOCT, we conducted mass spectrometry screen report specifically directly converts dinucleotide NADP+ into NAD+ NADPH NADH. Further, demonstrate Drosophila ortholog, Curled, same enzymatic We obtained 2.7 Å...
Double-stranded RNA (dsRNA), a hallmark viral material that activates antiviral interferon (IFN) responses, can appear in human cells also the absence of viruses. We identify phosphorothioate DNAs (PS DNAs) as triggers such endogenous dsRNA (endo-dsRNA). PS inhibit decay nuclear RNAs and induce endo-dsRNA via accumulation high levels intronic intergenic inverted retroelements (IIIR). IIIRs activate responses distinct from defense programs. do not turn on transcriptional RIG-I/MDA5/IFN...
For influenza A and B viruses to be infectious, they require eight viral RNA (vRNA) genome segments packaged into virions. efficient packaging, utilize cis-acting vRNA sequences, containing both non-coding protein coding regions of each segment. Whether have similar packaging signals is unknown. Here we show that at the 3' 5' ends virus segment 4 are required for with first 30 nt end essential this process. Synonymous mutation these led attenuation, an increase in defective particle...
Chemotaxis is migration along a specific chemical gradient1. Chemokines are chemotactic cytokines that promote cellular trafficking with anatomic and temporal specificity2. critical function of lymphocytes other immune cells can be quantitatively assessed in vitro. This manuscript describes methods permit the evaluation chemotaxis, both vitro vivo, for diverse cell types including lines native cells. The vitro, plate-based format permits comparison several conditions simultaneously...
RNA degradation by RNase L during 2-5A-mediated decay (2-5AMD) is a conserved mammalian stress response to viral and endogenous double-stranded (dsRNA). 2-5AMD onsets rapidly facilitates switch of protein synthesis from homeostasis production interferons (IFNs). To understand the mechanism this reprogramming, we examined in human cells. triggers polysome collapse characteristic translation initiation defect, but complexes ribosomes purified translation-arrested cells remain functional. Using...
Abstract Nocturnin (NOCT) is a rhythmically expressed protein that regulates metabolism under the control of circadian clock. It has been proposed NOCT deadenylates and metabolic enzyme mRNAs. However, in contrast to other deadenylases, purified lacks deadenylase activity. To identify substrate NOCT, we conducted mass spectrometry screen report specifically directly converts dinucleotide NADP + into NAD NADPH NADH. Further, demonstrate Drosophila ortholog, Curled, same enzymatic We obtained...
Chemotaxis is migration along a specific chemical gradient1. Chemokines are chemotactic cytokines that promote cellular trafficking with anatomic and temporal specificity2. critical function of lymphocytes other immune cells can be quantitatively assessed in vitro. This manuscript describes methods permit the evaluation chemotaxis, both vitro vivo, for diverse cell types including lines native cells. The vitro, plate-based format permits comparison several conditions simultaneously...