- Hydrogels: synthesis, properties, applications
- Marine Biology and Environmental Chemistry
- Marine Bivalve and Aquaculture Studies
- Aquaculture Nutrition and Growth
- Reproductive biology and impacts on aquatic species
- 3D Printing in Biomedical Research
- Coral and Marine Ecosystems Studies
- Algal biology and biofuel production
- Marine Sponges and Natural Products
- Reproductive Biology and Fertility
- Microplastics and Plastic Pollution
- Advanced Drug Delivery Systems
- Physiological and biochemical adaptations
- Marine and coastal plant biology
- Bone Tissue Engineering Materials
- Ovarian function and disorders
- Spaceflight effects on biology
- Ocean Acidification Effects and Responses
- Nanoparticle-Based Drug Delivery
- Aquaculture disease management and microbiota
- Animal Genetics and Reproduction
- Bacterial biofilms and quorum sensing
- biodegradable polymer synthesis and properties
- Electrospun Nanofibers in Biomedical Applications
- Polar Research and Ecology
A.V. Zhirmunsky National Scientific Center of Marine Biology Far Eastern Branch of the Russian Academy of Sciences
2013-2025
Far Eastern Branch of the Russian Academy of Sciences
2009-2020
Russian Academy of Sciences
2009-2014
Far Eastern Federal University
2012-2014
Far East University
2008
Heat stress and other factors cause the loss of endosymbiotic dinoflagellates by corals, is known as coral bleaching. Coral reef bleaching a global environmental problem. To better understand corals' responses adaptability to stressful conditions, we applied lipidomic approach in combination with cytometry microscopy study Acropora aspera under heat (32 °C) subsequent recovery. For eight days bleaching, lost 50% its symbiont population 100% after week It took 126 fully recover population,...
Here, we discuss the applicability of (methylenebis(salicylaldehyde)-MbSA) for fabrication stimuli-responsive N-carboxyethylchitosan (CEC) hydrogels with a tunable dissolution rate under physiological conditions. In comparison non-covalent salicylimine hydrogels, MbSA cross-linking via covalent bis('imine clip') and hydrophobic interactions allowed storage moduli > 1 kPa at ten-fold lower aldehyde/CEC molar ratio preservation pH- amino-acid responsive behavior. Although MbSA-cross-linked CEC...
Cross-linking chitosan at room and subzero temperature using a series of diglycidyl ethers glycols (DEs)—ethylene glycol (EGDE), 1,4-butanediol (BDDE), poly(ethylene glycol) (PEGDE) has been investigated to demonstrate that DEs can be more powerful alternative glutaraldehyde (GA) for fabrication biocompatible cryogels with tunable properties. Gelation was significantly slower than GA, allowing formation larger pores higher permeability, suitable flow-through applications cell culturing....
Here we demonstrate the possibility of using acyclic diethylacetal acetaldehyde (ADA) with low cytotoxicity for fabrication hydrogels via Schiff bases formation between chitosan and generated in situ from acetals acetate solution. This approach is more convenient than a direct reaction due to better commercial availability higher boiling point acetals. Rheological data confirmed intermolecular bonds solution after addition diethyl acetal at an equimolar NH2: ratio. The chemical structure...
The potential of chitosan and carboxymethyl (CMC) cryogels cross-linked with diglycidyl ether 1,4-butandiol (BDDGE) poly(ethylene glycol) (PEGDGE) have been compared in terms 3D culturing HEK-293T cell line preventing the bacterial colonization scaffolds. first attempts to apply for co-culturing bacteria human cells undertaken toward development new models host-pathogen interactions bioimplant-associated infections. Using a combination scanning electron microscopy, confocal laser flow...
Here, we have presented a new method of 1,1,3-triglycidyloxypropane (TGP) synthesis and investigated how cross-linker branching affects mechanical properties cytotoxicity chitosan scaffolds in comparison with those cross-linked using diglycidyl ethers 1,4-butandiol (BDDGE) poly(ethylene glycol) (PEGDGE). We demonstrated that TGP is an efficient for at subzero temperature TGP:chitosan molar ratios from 1:1 to 1:20. Although the elasticity increased following order cross-linkers PEGDGE >...
Abstract The symbiotic relationships between coral animal host and autotrophic dinoflagellates are based on the mutual exchange tight control of nutritional inputs supporting successful growth. corals Sinularia heterospiculata Acropora aspera were cultivated using a flow-through circulation system supplying seawater during cold warm seasons year, then sorted into cells symbionts subjected to phylogenetic, morphological, advanced lipid analyses. Here we show, that lipidomes Cladocopium C1/C3...
Although diglycidyl ethers of glycols (DEs)—FDA-approved reagents for biomedical applications—were considered unsuitable the fabrication chitosan (CH) hydrogels and cryogels, we have recently shown that CH cross-linking with DEs is possible, but its efficiency depends on nature acid used to dissolve pH. To elucidate origin low interactions in acetic solutions, put forward two hypotheses: (i) are consumed a side reaction acid; (ii) DE chain length strongly affects probability cross-linking....
Macroporous scaffolds (cryogels) for the 3D cell culturing of colorectal cancer micro-tumors have been fabricated by cross-linking chitosan and carboxymethyl (CMC) with 1,4-butandiol diglycidyl ether (BDDGE) under subzero temperature. Due to different intrinsic properties reactivity CMC same conditions, Young's moduli swelling permeable HCT 116 cells cryogels varied in broad range 3-41 kPa 3500-6000%, respectively. We demonstrated that morphology can be controlled via selection polymer...