A5054594219- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Carcinogens and Genotoxicity Assessment
- Fungal and yeast genetics research
- PARP inhibition in cancer therapy
- Protist diversity and phylogeny
- Epigenetics and DNA Methylation
- Chromosomal and Genetic Variations
- Polyomavirus and related diseases
- Genetic factors in colorectal cancer
- Plant Genetic and Mutation Studies
- Cancer-related gene regulation
- Virus-based gene therapy research
- Bacterial Genetics and Biotechnology
- RNA modifications and cancer
- Radiation Therapy and Dosimetry
- RNA Interference and Gene Delivery
- Genetic Neurodegenerative Diseases
- DNA and Nucleic Acid Chemistry
- Enzyme Structure and Function
- Microtubule and mitosis dynamics
- Tissue Engineering and Regenerative Medicine
- Plant tissue culture and regeneration
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2007-2024
Université Paris Cité
2021-2024
CEA Paris-Saclay
2021-2024
Inserm
2021-2024
Université Paris-Saclay
2018-2024
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2007-2023
Institut de Radiobiologie Cellulaire et Moléculaire
2010-2023
Délégation Paris 7
2021
Direction de la Recherche Fondamentale
2017-2018
Laboratoire de Génétique Cellulaire
2013
Laurent Maloisel and Jean-Luc Rossignol Institut de Génétique et Microbiologie, Université Paris-Sud, 91405 Orsay Cedex, France
Damage tolerance mechanisms mediating damage-bypass and gap-filling are crucial for genome integrity. A major damage pathway involves recombination is referred to as template switch. Template switch intermediates were visualized by 2D gel electrophoresis in the proximity of replication forks X-shaped structures involving sister chromatid junctions. The homologous factor Rad51 required formation/stabilization these intermediates, but its mode action remains be investigated. By using a...
DNA polymerases play a central role during homologous recombination (HR), but the identity of enzyme(s) implicated remains elusive. The pol3-ct allele gene encoding catalytic subunit polymerase delta (Poldelta) has highlighted for this in meiotic HR. We now address ubiquitous Poldelta HR somatic cells. find that affects conversion tract length mitotic whether event is initiated by single-strand gaps following UV irradiation or site-specific double-strand breaks. show effects on are...
Suppressors of the methyl methanesulfonate sensitivity Saccharomyces cerevisiae diploids lacking Srs2 helicase turned out to contain semidominant mutations in Rad5l, a homolog bacterial RecA protein. The nature these was determined by direct sequencing. 26 characterized were single base substitutions leading amino acid replacements at 18 different sites. great majority sites (75%) are conserved family RecA-like proteins, and 10 them affect corresponding acids that probably directly involved...
Significance During meiosis, programmed chromosome breakage and subsequent double-stranded DNA (dsDNA) break repair help ensure correct segregation promote genetic diversity of the progeny. In budding yeast, which utilizes meiotic recombination pathways conserved in mice humans, majority crossovers are initiated through formation a Holliday junction, requires endonuclease activity Mlh1-Mlh3 mismatch factor to be resolved exclusively into crossover product. Here, we combined structural...
Abstract A screen for mutants of budding yeast defective in meiotic gene conversion identified a novel allele the POL3 gene. encodes catalytic subunit DNA polymerase δ, an essential involved genomic replication. The new allele, pol3-ct, specifies protein missing last four amino acids. pol3-ct shows little or no defect replication, but displays reduction length tracts and decrease crossing over. We propose model which synthesis determines strand exchange intermediates influences their...
Homology search and strand exchange mediated by Rad51 nucleoprotein filaments are key steps of the homologous recombination process. In budding yeast, Rad52 is main mediator filament formation, thereby playing an essential role. The current model assumes that formation requires interaction between Rad51. However, we report here mutations disrupt this do not affect γ-ray- or HO endonuclease-induced gene conversion frequencies. vivo in vitro studies confirmed affected these mutations. Instead,...
Replicative DNA polymerases cannot insert efficiently nucleotides at sites of base lesions. This function is taken over by specialized translesion synthesis (TLS) to allow replication completion in the presence damage. In eukaryotes, Rad6- and Rad18-mediated PCNA ubiquitination lysine 164 promotes recruitment TLS polymerases, allowing cells cope with However, several studies showed that can be recruited also absence ubiquitination. We hypothesized stability interactions between polymerase δ...
The budding yeast Srs2 is the archetype of helicases that regulate several aspects homologous recombination (HR) to maintain genomic stability. inhibits HR at replication forks and prevents high frequencies crossing-over. Additionally, sensitivity DNA damage synthetic lethality with mutants are phenotypes can only be attributed another role Srs2: elimination lethal intermediates formed by proteins. To shed light on these intermediates, we searched for mutations bypass requirement in repair...
The bypass of DNA lesions that block replicative polymerases during replication relies on damage tolerance pathways. error-prone translesion synthesis (TLS) pathway depends specialized incorporate nucleotides in front base lesions, potentially inducing mutagenesis. Two error-free pathways can the lesions: template switching pathway, which uses sister chromatid as a template, and homologous recombination (HR), also use chromosome template. balance between controls mutagenesis level....
Homologous recombination (HR) depends on the formation of a nucleoprotein filament recombinase Rad51 to scan genome and invade homologous sequence used as template for DNA repair synthesis. Therefore, HR is highly accurate crucial stability. controlled by positive negative factors. In Saccharomyces cerevisiae, mediator protein Rad52 catalyzes stabilizes them, mostly counteracting disruptive activity translocase Srs2. Srs2 essential avoid toxic filaments, revealed Srs2-deficient cells. We...
Abstract Homologous recombination (HR) is essential for the repair of DNA double-strand breaks and restart stalled replication forks. A critical step in HR formation Rad51 nucleofilaments, which perform homology search strand invasion a homologous sequence required synthesis. In yeast Saccharomyces cerevisiae , Rad52 facilitates nucleofilament by mediating loading onto ssDNA counteracting dissociation filaments translocase Srs2. The molecular basis these two functions remains unclear. Our...
Abstract The bypass of DNA lesions that block replicative polymerases during replication relies on damage tolerance pathways. error-prone translesion synthesis (TLS) pathway depends specialized incorporate nucleotides in front base lesions, potentially inducing mutagenesis. Two error-free pathways can the lesions: template switching pathway, which uses sister chromatid as a template, and homologous recombination (HR), also use chromosome template. balance between controls mutagenesis level....
Abstract Homologous recombination (HR) depends on the formation of a nucleoprotein filament recombinase Rad51 to scan genome and invade homologous sequence used as template for DNA repair synthesis. Therefore, HR is highly accurate crucial stability. controlled by positive negative factors. In Saccharomyces cerevisiae , mediator protein Rad52 catalyzes stabilizes them, mostly counteracting disruptive activity translocase Srs2. Srs2 essential avoid toxic filaments, revealed Srs2-deficient...