Breast cancer is the most frequently diagnosed disease in women and remains a leading cause of cancer-related deaths worldwide. The small non-coding RNA molecule, MicroRNA-208 (miR-208), plays crucial role development advancement numerous malignancies, including breast cancer. This study aimed to investigate miR-208 potential as an oncogene by examining its impact on cell motility epithelial-mesenchymal transition (EMT). Tissue samples from 25 Iraqi with malignant benign were analysed. Total...

Background: Biomarkers that monitor treatment efficacy could be very useful for early response evaluation, therapy direction, and prognosis prediction. MicroRNAs (miRNAs), a type of noncoding RNA is only 22 nucleotides long regulate genes after transcription has taken place. miRNAs are essential regulators cancer biology involved in the regulation key processes such as cell proliferation, apoptosis, migration, metastasis. Methods: This study included 100 women with breast (BC) who attended...

Pseudomonas aeruginosa is a common and major opportunistic human pathogen, its causes many dangersinfectious diseases due to death in some timesex: cystic fibrosis , wounds inflammation burns urinary tract infection other infections otitis external Endocarditis nosocomial also blood (Bacteremia). thereforebecomes founding fast exact identification of P. aeruginosafrom samples culture very important.However, this species may be problematic the marked phenotypic variabilitydemonstrated by...

Breast cancer (BC) stands as the most prevalent form of carcinoma among women, ranking second leading cause cancer-related mortality in female population. The objective this study is to assess expression miR-10b and determine its diagnostic prognostic significance breast patients across various disease stages. investigation was carried out Baghdad at Oncology Teaching Hospital within Medical City Unit Al-Yarmouk Hospital. A total 150 samples were included divided into two groups: blood group...

Breast cancer (BC) is a multifaceted disease characterized by different molecular subtypes and stages. MicroRNAs (miRNAs)are increasingly becoming recognized as valuable tools for predicting identifying persons with BC, particularly miR-195. This study aimed to evaluate the expression of miR-195 in BC patients its correlation demographic clinical parameters. The involved 60 female healthy controls. Demographic analysis showed that 65% have family history BC. Invasive ductal carcinoma (IDC)...

Introduction and Aim: Breast cancer is a complex disease one of the most common cancers among women. linked to number inherited gene alterations which could either initiate breast tumors or be involved in multistage process development. In this study we aimed genotype resistin (RETN) determine effects RETN single nucleotide polymorphisms (rs3219175, rs34788323) Iraqi women.
 
 Materials Methods: This included 70 (35 malignant 35 benign) patients with breast-cancer free Blood...

One of the most deadly diseases that affect women globally is breast cancer. A variety factors contribute to onset and spread disease. Major them involve changes in expression certain genes, growing evidence suggesting (microRNAs) can control genes act as recent technique for predicting diagnosing BC patients. The objective study correlations both tumor markers with clinic pathological features patients by circulating serum marker miRNA-21 measured using quantitative real time-PCR 50 at...

Breast cancer is a heterogeneous disease defined by molecular types and subtypes. It constitutes the most commonly-diagnosed leading cause of death in women worldwide, according to International Agency for Research on Cancer (IARC) World Reports 2020. The study aimed evaluate miR-146 miR-196 expression level their association with ca15-3 serum participants diagnosed breast cancer. There were 105 samples, three groups 35 fresh blood samples FFPE Tissue which collected as malignant, benign...

Background: Elevated levels of microRNAs have been linked to breast cancer and other types cancer. It has discovered that miRNA-199a-5p overexpression contributes the epithelial-mesenchymal transition (EMT) in Objective: To examine expression microRNA 199a-5p tissues from both benign malignant cancers. Methods: We use 50 FFPE tissue samples divided into two groups based on their histology cytological characteristics. The first group comprised 25 classified as benign, while second malignant....

Breast cancer (BC) is a prevalent malignancy among women, ranking as the second most commonly diagnosed globally. Notably, substantial proportion of breast cancer-related fatalities, up to 90 percent, are attributed development distant organ metastases. While Cadherin1 (CDH1) has conventionally been considered tumor-suppressor gene in research, recent investigations have unequivocally revealed that both CDH1 and its encoded E-cadherin exhibit oncogenic characteristics. The primary focus this...

Breast cancer (BC) is a genetic disease in the mammary glands' ducts and lobules, with ductal cancers comprising most of malignancies. Biomarkers can provide an assessment diagnosis prediction. The study aims to compare expression serum (miR-21-5p) CA 15-3 Iraqi population as more efficient biomarkers, then checked MiRNA-21 main characters biomarker comparison (CA15-3) levels. Circulating miRNA-21 was measured using (the quantitative Real Time-PCR technique) 50 patients at various stages...

The study included 40 Iraqi women diagnosed as breast cancer patients who attended the Nuclear Medicine Hospital in Baghdad and 60 apparently healthy control .Genomic DNA extraction was performed using proteinase K / SDS method. Multiplex PCR techniques were followed to amplify genes specific primers. for detection of gstm1, gstt1 albumin gene a control. Genetic analysis showed that percentage deletions 75% (gstm1 27.5%, null genotype 32.5 % 15% gstt1) versus 10 samples (5 gstt1, 3.33% gstm1...

In this search, a new bioluminescent technique was proved for pyrophosphate which employed to single- nucleotide polymorphism (SNP) diagnosis using one-base extension reaction. Four Mycobacterium tuberculosis genes were chosen (Rpob, InhA, KatG, GyrA) genes. Fifty-four specimens used in study fifty-three as drug-resistant by The Iraqi Institute of Chest and Respiratory Diseases Baghdad., also one specimen negative control. procedure assay follows. A specific primer within each aliquot owning...

In this search, a new pyrophosphate technique was proved. The employed to single- nucleotide polymorphisms (SNPs), which diagnosis using one-base extension reaction. Three Mycobacterium tuberculosis genes were chosen (Rpob, InhA, KatG) genes. Fifty-four specimens used in study fifty-three proved as drug-resistant by Iraqi Institute of Chest and Respiratory Diseases Baghdad.; also one specimen negative control. steps specific primer within each aliquot that has short 3-OH end the base target...