A5026456188- Service-Oriented Architecture and Web Services
- Advanced Software Engineering Methodologies
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Context-Aware Activity Recognition Systems
- Software System Performance and Reliability
- Business Process Modeling and Analysis
- Human Mobility and Location-Based Analysis
- Personal Information Management and User Behavior
- RNA Research and Splicing
- Access Control and Trust
- RNA and protein synthesis mechanisms
- Renal and related cancers
- Pancreatic function and diabetes
- Reproductive Biology and Fertility
- Monoclonal and Polyclonal Antibodies Research
- Biomedical Ethics and Regulation
- Nuclear Structure and Function
- Multi-Agent Systems and Negotiation
- Data Management and Algorithms
- Cloud Computing and Resource Management
- Viral Infectious Diseases and Gene Expression in Insects
- Genomics and Chromatin Dynamics
- RNA Interference and Gene Delivery
Swinburne University of Technology
2014-2025
Data61
2023
Commonwealth Scientific and Industrial Research Organisation
2023
Harvard University
2014-2023
Harvard Stem Cell Institute
2022
Chittagong University of Engineering & Technology
2021
International Islamic University Chittagong
2021
Macquarie University
2021
Eindhoven University of Technology
2019
Institute of Medical Biology
2007-2017
Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene (neo) and human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Two cloned transfectants population (G418)-resistant cells used as donors nuclear transfer to enucleated oocytes. Six transgenic lambs liveborn: Three produced from contained neo transgenes, whereas three uncloned only. Somatic can therefore be subjected genetic manipulation vitro...
The nuclear lamina consists of A- and B-type lamins. Mutations in LMNA cause many human diseases, including progeria, a premature aging syndrome, whereas LMNB1 duplication causes adult-onset autosomal dominant leukodystrophy (ADLD). is reduced cells from progeria patients, but the significance this reduction unclear. In paper, we show that protein levels decline senescent dermal fibroblasts keratinocytes, mediated by transcription inhibition messenger ribonucleic acid (RNA) translation...
Synthetic RNAs coding for chicken lysozyme, calf preprochymozin and Xenopus globin were transcribed invitro using Sp6 RNA polymerase. The effects of capping adding a poly(A) tail on the stability, movement translation these in oocytes was examined. Capping polyadenylation increased stability transcripts, with at least 40% remaining intact 48h after injection into oocytes. Capped transcripts moved more rapidly than either capped + or naturally occurring mRNAs. translational efficiency most...
A method is described for the rapid preparation of plasmid DNAs molecular weight up to 14 × 106. This involves chromatography, at room temperature, bacterial cleared lysates on hydroxyapatite in presence high concentrations phosphate and urea. All detectable protein RNA contamination DNA removed by this procedure conformation unaffected. Less than 0.5% chromosomal present purified even can be if necessary a simple extention include heat-denaturation step. The extremely amenable large-scale...
Human embryonic stem cells (hESCs) regularly acquire nonrandom genomic aberrations during culture, raising concerns about their safe therapeutic application.The International Stem Cell Initiative identified a copy number variant (CNV) amplification of chromosome 20q11.21 in 25% hESC lines displaying normal karyotype.By comparing four cell paired for the presence or absence this CNV, we show that those containing amplicon have higher population doubling rates, attributable to enhanced...
Human embryonic stem (hES) cells represent a potentially unlimited source of transplantable beta-cells for the treatment diabetes. Here we describe differentiation strategy that reproducibly directs HES3, an National Institutes Health (NIH)-registered hES cell line, into pancreatic endocrine lineage. HES3 are removed from their feeder layer and cultured as embryoid bodies in three-dimensional matrix presence Activin A Bmp4 to induce definitive endoderm. Next, growth factors known promote...
Corneal endothelium-associated corneal blindness is the most common indication for transplantation. Restorative transplant surgery only option to reverse blindness, but a global shortage of donor material remains an issue. There are immense clinical interests in development alternative treatment strategies alleviate current reliance on materials. For such endeavors, ex vivo propagation human endothelial cells (hCECs) required, methodology lacks consistency, with expanded hCECs losing...
Aging | doi:10.18632/aging.100503. Chung-Wah Siu, Yee-Ki Lee, Jenny Chung-Yee Ho, Wing-Hon Lai, Yau-Chi Chan, Kwong-Man Ng, Lai-Yung Wong, Ka-Wing Au, Yee-Man Lau, Jinqiu Zhang, Kenneth Weijian Lay, Alan Colman, Hung-Fat Tse