A5047008696- Neuroscience and Neuropharmacology Research
- Neuroscience of respiration and sleep
- Zebrafish Biomedical Research Applications
- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Sleep and Wakefulness Research
- Neuroendocrine regulation and behavior
- Metabolism and Genetic Disorders
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neural Engineering
- Neurobiology and Insect Physiology Research
- Glaucoma and retinal disorders
- Retinal Development and Disorders
- Advanced biosensing and bioanalysis techniques
- Medicinal Plants and Neuroprotection
- Photoreceptor and optogenetics research
- bioluminescence and chemiluminescence research
- Muscle metabolism and nutrition
- Meat and Animal Product Quality
- Apelin-related biomedical research
- Diet and metabolism studies
- Neurogenesis and neuroplasticity mechanisms
- Advanced Biosensing Techniques and Applications
- Infant Development and Preterm Care
- Muscle Physiology and Disorders
Institut de Biologie Paris-Seine
2017-2024
Université Paris-Seine
2023
Sorbonne Université
2013-2017
Inserm
2017
Centre National de la Recherche Scientifique
1995-2017
Neurosciences Paris-Seine
2017
Centre National pour la Recherche Scientifique et Technique (CNRST)
2013
National Institute of Neurological Disorders and Stroke
1998-2009
Institut de l’Elevage
2009
National Institutes of Health
1998-2009
We examined the ability of isolated lumbosacral spinal cord neonatal mouse (P0–7) to generate rhythmic motor activity under several different conditions. In absence electrical or pharmacological stimulation, we recorded patterns spontaneous ventral root depolarization and discharge. Spontaneous, alternating discharge between contralateral roots could occur two three times over a 10-min interval. also observed other patterns, including left-right synchrony restricted one side cord. Trains...
Mammalian spinal motoneurons are considered to be output elements of the cord that generate exclusively cholinergic actions on Renshaw cells, their intraspinal synaptic targets. Here, we show antidromic stimulation motor axons evokes depolarizing monosynaptic potentials in cells depressed, but not abolished, by antagonists. This residual potential was abolished 2-amino-5-phosphonovaleric acid and 6-cyano-7-nitroquinoxaline-2,3-dione. In presence antagonists, axon triggered locomotor-like...
The availability of genetic tests to detect different mutations in the myostatin gene allows identification heterozygous animals and would warrant superiority these for slaughter performance if this is confirmed. Thus, 2 gene, Q204X nt821, were studied 3 French beef breeds program Qualvigène. This work was done with 1,114 Charolais, 1,254 Limousin, 981 Blonde d'Aquitaine young bulls from, respectively, 48, 36, 30 sires slaughtered from 2004 2006. In addition usual carcass traits recorded at...
We used calcium imaging to visualize the spatiotemporal pattern of motoneuron activity during dorsal root-evoked locomotor-like bursting in lumbosacral spinal cord neonatal mouse. Dorsal root stimuli elicited a tonic discharge motoneurons on which alternating left-right rhythmic discharges were superimposed. Both and components could be recorded optically from populations labeled with calcium-green dextran. Optical electrical recordings revealed that signals different parts lumbar (L1, L2)...
Calcium imaging of neural network function has been limited by the extent tissue labeled or time taken for labeling. We now describe use electroporation-an established technique transfecting cells with genes-to load neurons calcium-sensitive dyes in isolated spinal cord neonatal mouse vitro. The were injected subdurally, intravascularly, into central canal. This results rapid and extensive labeling their processes at all depths cord, over a rostrocaudal determined position size electrodes....
To further understand the excitatory effects of motoneurons on spinal network function, we investigated entrainment disinhibited rhythms by ventral root (VR) stimulation in neonatal mouse cord. A brief train stimuli applied to a VR triggered bursting reliably 31/32 experiments. The same roots that entrained could also produce locomotor-like activity with similar probability when was not disinhibited. ability entrain rhythm persisted nicotinic and muscarinic cholinergic antagonists but...
The β-strands of GFP form a rigid barrel that protects the chromophore from external influence. Herein, we identified specific mutations in β-strand 7 render sensitive to interactions with another protein domain. In process converting FRET-based kinase A (PKA) sensor AKAR2 into single-wavelength PKA containing and quencher, discovered quencher was not required response relied on changes intrinsic fluorescence. fluorescence intensity lifetime conformational PKA-sensing addition, sensors...
It has recently been demonstrated that motoneurons in neonatal rodents release an excitatory amino acid, addition to acetylcholine, from their central terminals onto Renshaw cells. Although the function of this acid is not understood, it may mediate actions motor axon stimulation on spinal networks. Stimulation axons ventral roots or muscle nerves can activate locomotor pattern generator entrain bursting disinhibited cord. Both these effects persist presence cholinergic antagonists and are...
Abstract Previous studies have reported that the α 1 ‐adrenergic system can activate spinal rhythm generators belonging to central respiratory network. In order analyse effects on both cranial and motoneuronal activity, phenylephrine (1–800 μ m ) was applied in vitro preparations of neonatal rat brainstem–spinal cord. High concentration superfusion exerted multiple cervical outputs (C2–C6), consisting a lengthening period an increase inspiratory burst duration. Furthermore, 55% cases slow...
GABAergic interneurons are known to control activity balance in physiological conditions and coordinate hippocampal networks during cognitive tasks. In temporal lobe epilepsy interneuron loss consecutive network imbalance could favor pathological hypersynchronous epileptic discharges. We tested this hypothesis mice by vivo unilateral epileptogenic kainate lesion followed vitro recording of extracellular potentials patch-clamp from GFP-expressing CA3, an optimized chamber. Slices lesioned...
Knowing when seizures occur may help patients and can also provide insight into epileptogenesis mechanisms. We recorded over periods of several days in the Genetic Absence Epileptic Rat from Strasbourg (GAERS) model absence epilepsy, while we monitored behavioral activity with a combined head accelerometer (ACCEL), neck electromyogram (EMG), electrooculogram (EOG). The three markers consistently discriminated between states rest. Both GAERS control Wistar rats spent more time rest (55–66%)...
Behavioural Pharmacology of Excitatory Amino Acids and their Antagonists Marseille, France, 20–23 September 1995: PDF Only