A5030868264- Osteoarthritis Treatment and Mechanisms
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Knee injuries and reconstruction techniques
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Nerve injury and regeneration
- Bone Metabolism and Diseases
- Silk-based biomaterials and applications
- Angiogenesis and VEGF in Cancer
- Cellular Mechanics and Interactions
- Inflammatory Bowel Disease
- Bone Tissue Engineering Materials
- Pancreatic and Hepatic Oncology Research
- Proteoglycans and glycosaminoglycans research
- Anorectal Disease Treatments and Outcomes
- Alkaline Phosphatase Research Studies
- Neuroscience and Neural Engineering
- Sexual function and dysfunction studies
- RNA Research and Splicing
- Diverticular Disease and Complications
- Pluripotent Stem Cells Research
- Bone health and osteoporosis research
- 3D Printing in Biomedical Research
- Pancreatitis Pathology and Treatment
Johns Hopkins Medicine
2022-2024
Johns Hopkins University
2022-2024
City University of New York
2017-2022
City College of New York
2018-2022
City College
2018-2020
The University of Texas Health Science Center at Houston
2016
Brown Foundation
2015-2016
The Wallace H. Coulter Department of Biomedical Engineering
2010-2013
Georgia Institute of Technology
2010-2013
Emory University
2011
Adult mesenchymal stem cells (MSCs) hold great promise for regenerative medicine because of their self-renewal, multipotency, and trophic immunosuppressive effects. Due to the rareness high heterogeneity freshly isolated MSCs, extensive in-vitro passage is required expand populations prior clinical use; however, senescence usually accompanies can potentially affect MSC characteristics functionality. Therefore, a thorough characterization variations in phenotype differentiation potential...
Multipotent mesenchymal stem cells (MSCs) represent a great candidate for various clinical applications including regenerative medicine. However, aging both in vivo and vitro can significantly compromise MSC characteristics performance. This paper highlights current thoughts on senescence-induced damage to MSCs that should be considered prior their use regeneration of different cells, tissues or organs.
Perianal fistulas (PAFs) represent a severe complication of Crohn's disease (CD). Despite the advent biologic and small-molecule therapeutics for luminal disease, PAFs in CD (CD-PAF) are relatively resistant to treatment, with less than 50% responding any therapy. We report an injectable, biodegradable, mechanically fragmented nanofiber-hydrogel composite (mfNHC) loaded adipose-derived stem cells (ADSCs) treatment rat model CD-PAF. The ADSC-loaded mfNHC results higher degree healing when...
Mechanotransduction in neural cells involves multiple signaling pathways that are not fully understood. Differences lineage and maturation state suggested causes for conflicting reports on cell mechanosensitivity. To optimize matrices use stem therapy treatments transplanting human induced pluripotent derived (hNSC) into lesions after spinal cord injury, the effects of Young's Modulus changes hNSC behavior must be The present study utilizes polyethylene glycol hydrogels containing a...
Abstract Controlled differentiation of mesenchymal stem cells (MSCs) into the chondrogenic lineage is crucial for in vitro generation neocartilage, yet achieving it remains challenging. Traditional protocols MSC using exogenous inductive molecules, such as transforming growth factor-β, fall short meeting needs clinical applications because they yield differentiated that exhibit hypertrophic characteristics and subsequently facilitate endochondral bone formation. The objective current study...
Application of mechanical stimulation, using dynamic bioreactors, is considered an effective strategy to enhance cellular behavior in load-bearing tissues. In this study, two types perfusion mode (direct and free flow) are investigated terms the biosynthetic activities chondrocytes grown collagen sponges by assessment cell proliferation rate, matrix production, tissue morphology. Effects duration preculture conditioning further determined. Our results have demonstrated that both bovine...
Abstract Functional microgels are preferred stem cell carriers due to the ease of delivery through minimally invasive injection and seamless integration with surrounding host tissue. A biostimulatory nanofiber‐hydrogel composite (NHC) has been previously developed covalently crosslinking a hyaluronic acid hydrogel network surface‐functionalized poly (ε‐caprolactone) nanofiber fragments. The NHC mimics microarchitecture native soft tissue matrix, showing enhanced infiltration,...
Achievement of viable engineered tissues through in vitro cultivation bioreactor systems requires a thorough understanding the complex interplay between hydrodynamic forces and biochemical cues such as serum. To this end, chondrocyte-seeded constructs were cultured under continuous fluid-induced shear with reduced serum content (0%–2%, v/v), which was partially or completely replaced by potential substitute, insulin-transferrin-selenium, to minimize deleterious effects associated use culture...
Successful tissue-engineering strategies for cartilage repair must maximize the efficacy of chondrocytes within their limited life span. To that end, combination exogenous growth factors with mechanical stimuli holds promise development clinically relevant tissue substitutes. The current study aimed to determine whether incorporation transient exposure into a hydrodynamic bioreactor system can improve functional maturation tissue-engineered cartilage. Chondrocyte-seeded polyglycolic acid...
Abstract The interplay between biomaterials and host immune responses critically determines outcomes in tissue restoration. Recent studies suggest that physicochemical properties of materials can dictate pro‐regenerative versus pro‐fibrotic have begun to define the key cell types signals governing these divergent effects. This emerging understanding enables engineering regenerative capable functional restoration situ. An injectable nanofiber‐hydrogel composite (NHC) microparticles are...
Matrix-assisted chondrocyte transplantation (MACT) is of great interest for the treatment patients with cartilage lesions. However, roles matrix properties in modulating tissue integration during MACT recovery have not been fully understood. The objective this study was to uncover effects substrate mechanics on implanted chondrocyte-laden hydrogels native tissues. To end, agarose Young's moduli ranging from 0.49 kPa (0.5%, w/v) 23.08 (10%) were prepared and incorporated into an vitro human...
Achievement of viable engineered tissues through in-vitro cultivation in bioreactor systems requires a thorough understanding the complex interplay between mechanical forces and biochemical cues. Briefly, bioreactors have been employed to impart stimuli support tissue growth development. Continuous fluid-induced shear stress, for example, has shown influence morphology properties cartilage.1 Fluid flow enhances mass transfer mechanisms simultaneously provides across or construct emulate that...
Abstract This paper aims to capture the behavior of bone gene regulation network (GRN) portion that controls mineralization according stiffness. We hypothesized epistatic genetic interactions follow a system nonlinear differential equations. To test our hypothesis, we (i) adapted previously published model from being continuously time-dependent stiffness-dependent, (ii) performed in-vitro experiments culturing cells on different stiffness, and (iii) optimized based experimental results. Our...