A5042912377- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Reproductive System and Pregnancy
- Immune Cell Function and Interaction
- Toxin Mechanisms and Immunotoxins
- Toxoplasma gondii Research Studies
- Transgenic Plants and Applications
- Cytomegalovirus and herpesvirus research
- Urticaria and Related Conditions
- Autoimmune and Inflammatory Disorders Research
- Parasites and Host Interactions
- Pineapple and bromelain studies
- Polysaccharides and Plant Cell Walls
- Ubiquitin and proteasome pathways
- Synthesis and Biological Evaluation
- Parvovirus B19 Infection Studies
Universidade de Brasília
2014-2021
Brazilian Society of Tropical Medicine
2018
Faculdade Brasil
2016
Fundação Oswaldo Cruz
2003-2008
Universidade Federal da Bahia
2003-2007
Leishmaniasis remains a serious public health problem in several parts of the developing world. Effective prophylactic measurements are hampered by imprecise comprehension different aspects disease, including its immunoregulation. A better immunoregulation human VL may be useful both for designing and evaluating immunoprophylaxis.To explore immunoregulatory mechanisms, 20 visceral leishmaniasis (VL) patients were evaluated during active disease at periods up to one year after treatment...
Cutaneous leishmaniasis (CL) is a worldwide disease endemic in several regions of the globe. The hallmark CL skin ulcers likely driven by efforts immune system to control Leishmania growth. Cytokines, such as tumor necrosis factor (TNF) and interferon-gamma can progression animal models. Nevertheless, impact these cytokines ulcer outcome not well established humans. In this study, 96 patients from an area braziliensis were enrolled for follow-up study that consisted clinical immunological...
We evaluated the use of polymerase chain reaction (PCR) for diagnosis American cutaneous leishmaniasis (ACL) in an area Bahia, Brazil, where Leishmania braziliensis is endemic. DNA was detected 50 cases, yielding a positivity rate 100%, which higher than rates all other diagnostic methods studied—namely, Montenegro skin test, anti-Leishmania serological testing, and microscopic examination lesion biopsy specimens. These findings have led us to propose guidelines ACL that PCR as principal...
In this report, we describe an investigation of the effects Lutzomyia longipalpis sand fly salivary gland homogenates (SGH) on cytokine production and expression costimulatory molecules human monocytes, macrophages (Mphis), dendritic cells (DCs). SGH L. induced increase in interleukin-6 (IL-6), IL-8 IL-12p40 but a decrease tumor necrosis factor alpha IL-10 by lipopolysaccharida (LPS)-stimulated monocytes. We also examined surface Mphis, DCs. Whereas affected these monocytes it had little...
Abstract Dendritic cells (DCs) are of utmost importance in initiating an immune response and may also function as targets for pathogens. The presence pathogens inside DCs is likely to impair their functions thus, influence responses. In the present report, we evaluated impact Leishmania amazonensis during differentiation maturation human monocyte-derived DCs. live L. parasites DC led a significant decrease CD80 (92%) CD1a (56%) expression increase CD86 cell surface expression. Phenotypic...
Introduction: Diffuse Cutaneous Leishmaniasis (DCL) is a rare disease form associated with L. (L.) amazonensis in South America. It represents the "anergic" pole of American Tegumentary and explanation for its resistance to treatment remains elusive. We aimed study some possible immunological mechanisms involved poor DCL response by evaluating cell surface molecules obtained from patient flow cytometry. Case presentation: A 65-year-old who initially failed respond standard showed vacuolated...
The cell wall has a critical role in the host immune response to fungal pathogens. In this study, we investigated influence of two fractions dimorphic fungi Paracoccidioides brasiliensis (Pb) vitro generation monocyte-derived dendritic cells (MoDCs). Monocytes were purified from peripheral blood healthy donors and cultivated for seven days medium supplemented with IL-4 GM-CSF presence Pb fractions: alkali-insoluble F1, constituted by β-1,3-glucans, chitin proteins, alkali-soluble F2, mainly...
Generally, Trypanosoma cruzi infection in human is persistent and tends to chronicity, suggesting that the parasite evade immune surveillance by down regulating intracellular antigen processing routes. Within MHC class I pathway, majority of antigenic peptides are generated proteasome. However, upon IFN-γ stimulation, catalytic constitutive subunits proteasome replaced β1i/LMP2, β2i/MECL-1 β5i/LMP7 form immunoproteasome. In this scenario, we analyzed whether expression activity...
Visceral leishmaniasis is a severe form of the disease, caused by Leishmania infantum in New World. Patients present an anergic immune response that favors parasite establishment and spreading through tissues like bone marrow liver. On other hand, braziliensis causes localized cutaneous lesions, which can be self-healing some individuals. Interactions between host are essential to understand disease pathogenesis progression. In this context, dendritic cells (DCs) act as bridges connect...
Chagas disease is a debilitating and neglected caused by the protozoan Trypanosoma cruzi. Soon after infection, interactions among T. cruzi host innate immunity cells can drive/contribute to outcome. Dendritic (DCs), present in all tissues, are one of first immune interact with metacyclic trypomastigotes. Elucidating immunological events triggered immediately parasite-human DCs encounter may aid understanding role establishment infection course disease. Therefore, we performed transcriptomic...
Infection by Toxoplasma gondii represents a risk to fetal development during pregnancy. In this study, the serological profile of pregnant women from different regions Bahia, Brazil, was determined. Tests were conducted at LACEN-BA (Salvador, Brazil). The mean age 24.5 years (±7.41 years) and 56.4% positive for IgG negative IgM specific T. gondii. Seronegative represented 35.9% total (IgG- IgM-negative) inconclusive results comprised 4%. Differences observed in distinct regions. Therefore,...
Toward obtaining a more comprehensive understanding of factors governing activation and/or function during visceral leishmaniasis (VL), we have compared active disease (pre-treatment) versus post-chemotherapy immune response in VL patients by means ex vivo staining with different cell markers. Our results show that disease, the frequency T cells positive for CD25, CTLA-4 and CD45RO was significantly lower healthy controls, whereas Annexin V CD95 were higher. In all cases, chemotherapy able...
A leishmaniose visceral (LV) é uma zoonose endêmica na América Latina e 96% dos casos de LV são diagnosticados no Brasil. coinfecção HIV-LV tem sido diagnosticada em áreas endêmicas não para LV. O aumento do número coinfecções todo o mundo deve-se, parte, à coincidência das circulação desses organismos. Deve-se ressaltar que a concomitância duas infecções potencialmente deletéria, portanto associação dois patógenos constitui um desafio diagnóstico controle da interação entre Leishmania HIV...