The process of straw decomposition is dynamic and accompanied by the succession microbial decomposing community, which driven poorly understood interactions between microorganisms. Soil a complex ecological niche, soil microbiome can serve as source potentially active cellulolytic Here, we performed an experiment on de novo colonization oat community placing nylon bags with sterilized in pots filled chernozem incubating them for 6 months. aim was to investigate changes decomposer microbiota...

Data on the main properties of Histic Podzols in pine forests semi-hydromorphic landscapes middle taiga Komi Republic after forest fires are presented. A decrease topsoil horizon thickness by more than 7.6 times, an increase litter density 6 and a stock 4 times were observed postfire soil. There was carbon content pyrogenic (48%) upper part podzolic horizon—from 0.49 at control plot to 1.16% fire. The accumulation all studied trace metals (Cu—from 2.5 6.8 mg × kg−1; Zn—from 35.7 127.4...

Recycling plant matter is one of the challenges facing humanity today and depends on efficient lignocellulose degradation. Although many bacterial strains from natural substrates demonstrate cellulolytic activities, CAZymes (Carbohydrate-Active enZYmes) responsible for these activities are very diverse usually distributed among different bacteria in habitat. Thus, using microbial consortia can be a solution to rapid effective decomposition biomass. Four were isolated enrichment cultures...

Introduction Soil microbiome is a major source of physiologically active microorganisms, which can be potentially mobilized by adding various nutrients. To study this process, long-term experiment was conducted on the decomposition oat straw and leaf litter using soil as microbial inoculum. Methods Combined analyses enzymatic activity NGS data for 16S rRNA gene amplicon full metagenome sequencing were applied to taxonomic, carbohydrate-active enzyme (CAZy), polysaccharide utilization loci...

Abstract Localization of agriculture with the aim local food support has become a very urgent topic for Yamal region. The most fertile soils this region are sandy textured anthropogenically affected soils. Microbiomes from disturbed Nadym were studied using analysis 16S rRNA metagenomic libraries. It was shown that plant cover is driving force microbiome composition. Forest covered aeolian transfers quarry retaids typical forest following dominant bacterial phyla: Proteobateria,...

Twenty-two rhizobia strains isolated from three distinct populations (North Ossetia, Dagestan, and Armenia) of a relict legume Vavilovia formosa were analysed to determine their position within Rhizobium leguminosarum biovar viciae (Rlv). These bacteria are described as symbionts four plant genera Pisum, Vicia, Lathyrus, Lens the Fabeae tribe, which is considered be closest its last common ancestor (LCA). In contrast viciae, trifolii (Rlt) inoculate plants Trifolieae tribe. Comparison...

High-throughput 16S rRNA sequencing was performed to compare the microbiomes inhabiting two contrasting soil types-sod-podzolic and chernozem-and corresponding culturome communities of potentially cellulolytic bacteria cultured on standard Hutchinson media. For each type, soil-specific microorganisms have been identified: for sod-podzolic soil-Acidothermus, Devosia, Phenylobacterium Tumebacillus, chernozem soil-Sphingomonas, Bacillus Blastococcus. The dynamics differences between types bulk...

Restoration of anthropogenically disturbed soils is an urgent problem in modern ecology and soil biology. processes northern environments are especially important, due to the small amounts fertile land low levels natural succession. We analyzed microbiota, which one indicators succession process soil. Samples were obtained from three (self-overgrown reclaimed quarries), two undisturbed (primary secondary forests). Primary Forest had a well-developed profile, pH TOC (total organic carbon)...

Vavilovia formosa is a relict leguminous plant growing in hard-to-reach habitats the rocky highlands of Caucasus and Middle East, it considered as putative closest living relative last common ancestor (LCA) Fabeae tribe. Symbionts belonging to Rhizobium leguminosarum bv. viciae compose discrete group that differs from other strains, especially nucleotide sequences symbiotically specialised (sym) genes. Comparison genomes strains with reference composed R. isolated Pisum Vicia demonstrated...

Here we investigate the microbiomes of soil samples from Yamal Peninsula (the surroundings Salekhard city, Russian Federation) using a high-throughput sequencing approach.The main goal was to impact mining on soils within following regeneration, both during reclamation practice and natural self-growth.Several quarries were studied, engaged in sand, clay chromatic ores mining.The taxonomic analysis revealed 50 bacterial archaeal phyla; among dominant phyla were: Proteobacteria,...

Abstract Soil microbiome plays an important role in soil forming process as well functioning. This is especially relevant for initial stages of regeneration after strong anthropogenic impact (i.e., quarrying complexes). The study such areas crucial both understanding the forces driving formation and optimization reclamation techniques. aimed to investigate different ages on heaps limestone mines conditions boreal climate North-West Russia. analysis was performed using high-throughput...

More than 30 years have passed after the collapse of Soviet Union, and huge areas soil were left in a fallow state. The study microbiological status soils is an extremely urgent task because represent “hidden” food basket Eurasia. In this context, we studied influence land use type (pasture, vegetable garden, hayfield, or secondary afforestation) on key agrochemical parameters microbial biodiversity. All anthropogenically transformed included analysis showed increased humus content pH shift...

This work describes the microbial community structure of continuously revegetated chronosequence a former sand quarry, which demonstrates unique example nearly complete soil restoration in less than 100 years. Samples were collected at five time points (0, 3, 30, 70 years and mature soil) from entire set horizons, revealing history pedogenesis. Real-time PCR was applied to quantitatively describe bacterial archaeal communities. High-throughput sequencing V4 variable region 16S rRNA gene used...

The soil microbiome is composed of various communities that play an important role in the existence ecosystem services and sustainable functioning ecosystems under high anthropogenic loads. transition soils to a fallow state their subsequent transformation lead notable alteration taxonomic composition microbiome, impacting biochemical processes within its fertility levels. object this study comprised different-aged southern taiga vicinity Ban’kovo village, Leningrad region. method comprising...

The global climate crisis forces mankind to develop carbon storage technologies. “Ladoga” monitoring site is part of the Russian project “Carbon Supersites”, which aims methods and technologies control balance greenhouse gases in various ecosystems. This article shows condition soil vegetation cover polygon using example a typical southern taiga ecosystem Leningrad region (Russia). It revealed that soils here are significantly disturbed as result agrogenic impact, changes under influence...

The search for active cellulolytic consortia among soil microorganisms is of significant applied interest, while the dynamics formation such communities remain insufficiently studied. To gain insight into an community, experiment was designed to study colonization a sterile substrate (cellulose) by from two types: sod-podzolic and chernozem. achieve this, placed in incubated six months. assess microbiome dynamics, employed sequencing 16S rRNA ITS2 amplicon libraries at four-time points. It...

Soil microbiome makes a significant contribution to the implementation of ecosystem services, which are necessary for sustainable functioning ecosystems. Soils central Yakutia develop under dynamic physical and chemical conditions (long-term freezing/thawing processes, redistribution nutrients), ensures formation specific microbial community in natural anthropogenically transformed areas. The object study was natural, fallow, agricultural soils Yakutia. method high-throughput sequencing 16S...