A5001335437- Legume Nitrogen Fixing Symbiosis
- Microbial Community Ecology and Physiology
- Nematode management and characterization studies
- Agronomic Practices and Intercropping Systems
- Genomics and Phylogenetic Studies
- SARS-CoV-2 and COVID-19 Research
- Plant nutrient uptake and metabolism
- Protist diversity and phylogeny
- Immunotherapy and Immune Responses
- Viral gastroenteritis research and epidemiology
- Plant Disease Resistance and Genetics
- Soybean genetics and cultivation
- Agricultural Productivity and Crop Improvement
- Cassava research and cyanide
- COVID-19 Clinical Research Studies
- Microbial Metabolites in Food Biotechnology
- Spaceflight effects on biology
- Plant-Microbe Interactions and Immunity
- Chemical and Physical Studies
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Anaerobic Digestion and Biogas Production
- Hemoglobin structure and function
- Biofuel production and bioconversion
Gene Therapy Laboratory
2024
All-Russian Research Institute of Agricultural Microbiology
2016-2023
BG Group (United Kingdom)
2023
Federal Agency for Scientific Organizations
2017
Gram-stain-negative strains V5/3MT, V5/5K, V5/5M and V5/13 were isolated from root nodules of Vicia alpestris plants growing in the North Ossetia region (Caucasus). Sequencing partial 16S rRNA gene (rrs) four housekeeping genes (dnaK, gyrB, recA rpoB) showed that isolates V. most closely related to species Microvirga zambiensis (order Rhizobiales , family Methylobacteriaceae ) which was described for single isolate nodule Listia angolensis Zambia. Sequence similarities between -related M....
Gram-negative strains Tri-36, Tri-38, Tri-48T and Tri-53 were isolated from root nodules of the relict legume Oxytropis triphylla (Pall.) Pers. originating Zunduk Cape (Baikal Lake region, Russia). 16S rRNA gene sequencing showed that novel isolates phylogenetically closest to type Phyllobacterium sophorae LMG 27899T, brassicacearum 22836T, endophyticum 26470T bourgognense 22837T while similarity levels between most closely related strain P. 98.8-99.5 %. The recA glnII genes highest sequence...
Two Gram-stain-negative strains, RCAM04680T and RCAM04685, were isolated from root nodules of the relict legume Caragana jubata (Pall.) Poir. originating south-western shore Lake Khuvsgul (Mongolia). The 16S rRNA gene (rrs) sequencing data showed that these novel isolates belong to genus Bosea are phylogenetically closest type strains lathyri LMG 26379T, vaviloviae 28367T, massiliensis 26221T lupini 26383T (the rrs-similarity levels 98.7-98.8 %). recA strain highest sequence similarity B....
Combined inoculation of legumes with rhizobia and plant growth-promoting rhizobacteria or endophytes is a known technique for increasing the efficiency nitrogen-fixing symbiosis productivity. The aim this work was to expand knowledge about synergistic effects between commercial pasture root nodule bacteria relict legume species. Pot experiments were performed on common vetch (Vicia sativa L.) red clover (Trifolium pratense co-inoculated participation corresponding rhizobial strains (R....
Two bacterial strains Ach-343 and Opo-235 were isolated respectively from nodules of Miocene-Pliocene relict legumes Astragalus chorinensis Bunge Oxytropis popoviana Peschkova originated Buryatia (Baikal Lake region, Russia). For identification these the sequencing 16S rRNA (rrs) gene was used. Strain belonged to species Mesorhizobium japonicum, while strain identified as M. kowhaii (100 99.9% rrs similarity with type MAFF 303099T ICMP 19512T, respectively). Symbiotic genes well some that...
Diagnosis of specific T cell immunity to the antigenic determinants SARS-CoV-2 in patients seems be an increasingly important task due accumulation data about role immune response course coronavirus infection and clearance case secondary infection. Previously, we designed recombinant CorD_PS antigen for evaluation antiviral immunity, containing conservative immunogenic sequences structural proteins coronavirus. E. coli producing strain with stable expression was obtained. Aim present work is...
Twenty-two rhizobia strains isolated from three distinct populations (North Ossetia, Dagestan, and Armenia) of a relict legume Vavilovia formosa were analysed to determine their position within Rhizobium leguminosarum biovar viciae (Rlv). These bacteria are described as symbionts four plant genera Pisum, Vicia, Lathyrus, Lens the Fabeae tribe, which is considered be closest its last common ancestor (LCA). In contrast viciae, trifolii (Rlt) inoculate plants Trifolieae tribe. Comparison...
Vavilovia formosa is a relict leguminous plant growing in hard-to-reach habitats the rocky highlands of Caucasus and Middle East, it considered as putative closest living relative last common ancestor (LCA) Fabeae tribe. Symbionts belonging to Rhizobium leguminosarum bv. viciae compose discrete group that differs from other strains, especially nucleotide sequences symbiotically specialised (sym) genes. Comparison genomes strains with reference composed R. isolated Pisum Vicia demonstrated...
T-cell immune response is extremely important in protecting human body from diverse viral infections. It known that it can ensure clearance and complete recovery patients with humoral immunodeficiency. COVID-19 were found to have primarily directed against SARS-CoV-2 structural S, M, N, E proteins, nucleocapsid protein being most conserved. To assess immunity coronavirus infection evaluate an effectiveness of vaccine candidates, necessary develop optimal diagnostic antigen arising antigenic...
Ten rhizobial strains were isolated from root nodules of a relict legume Oxytropis popoviana Peschkova. For identification the isolates, sequencing rrs, internal transcribed spacer region, and housekeeping genes recA, glnII, rpoB was used. Nine fast-growing isolates Mesorhizobium-related; eight identified as M. japonicum one isolate belonged to kowhaii. The only slow-growing Bradyrhizobium sp. Two strains, Opo-242 strain Opo-243, same nodule. Symbiotic these searched throughout whole-genome...
A collection of rhizobial strains isolated from root nodules the narrowly endemic legume species Oxytropis erecta, O. anadyrensis, kamtschatica, and pumilio originating Kamchatka Peninsula (Russian Federation) was obtained. Analysis 16S ribosomal RNA gene sequence showed a significant diversity isolates belonging to families Rhizobiaceae (genus Rhizobium), Phyllobacteriaceae (genera Mesorhizobium, Phyllobacterium), Bradyrhizobiaceae Bosea, Tardiphaga). plant nodulation assay that only genus...
Novel coronavirus disease 2019, caused by the SARS-CoV-2, initiate humoral and cellular immune responses against diverse virus antigens. The assessment of SARS-CoV-2-specific is mainly carried out in routine practice determining specific immunoglobulins. However, high variability S-protein structure new genovariants SARS-CoV-2 lack correlation between antibodies CD8+ T-lymphocytes underlie false negative responses, mass immunity complicated due to complexity applying ELISPOT cytofluorometry...
Study of cellulose decomposition is an extremely important for the agricultural sciences, as straw one most affordable organic fertilizers.In this area, a large number research works devoted to biochemical mechanisms destruction, well study cellulolytic microorganisms' taxonomic diversity were conducted.However, composition microbial community was estimated only by methods based on cultivation and thus describing very small part soil microbiome.With advent «new generation sequencing»...
The phenomenon of rhizobial synergy was investigated to increase the efficiency nitrogen-fixing symbiosis alfalfa (Medicago varia Martyn), common vetch (Vicia sativa L.) or red clover (Trifolium pratense L.). These plants were co-inoculated with respective commercial strains Sinorhizobium meliloti RCAM1750, Rhizobium leguminosarum RCAM0626 R. RCAM1365 and Mesorhizobium japonicum Opo-235, M. Opo-242, Bradyrhizobium sp. Opo-243 kowhaii Ach-343 isolated from relict legumes Oxytropis popoviana...
Due to the development of pandemic and need for mass vaccination, next-generation vaccines against COVID 19 coronavirus infection is a major challenge medicine biotechnology. prevalence 19, it still urgent develop safe protective vaccine, especially one that stimulates T-cell immune response. This work presents design production DNA immunogen based on plasmid encoding hybrid protein containing immunogenic fragments structural proteins SARS-CoV 2 β-coronavirus. As result, vector construct...
Nodule bacteria are presented as models for developing the major problems of evolutionary genetics – analysis trade-off between macro- and microevolution, progressive adaptive evolution, individual cooperative adaptations.
A collection of rhizobial strains isolated from root nodules the narrowly endemic legume species Oxytropis erecta, O. anadyrensis, kamtschatica and pumilio growing on Kamchatka Peninsula (Russian Federation) was obtained. Analysis 16S rRNA gene sequence showed a significant diversity isolates belonging to families Rhizobiaceae (Rhizobium), Phyllobacteriaceae (Mesorhizobium, Phyllobacterium) Bradyrhizobiaceae (Bosea, Tardiphaga). Pairs taxonomically different in various combinations were some...
In this article, we report the complete genome sequences of Massilia sp. strains B-10 (RCAM05335) and H-1 (RCAM05339), which were isolated from water Dal'nee Verkhnee Lake in Shulgan-Tash cave Russia (53°2'0″N, 57°3'0″E). The obtained using an Oxford Nanopore Technologies MinION system.
The Shulgan-Tash (Kapova) cave is a unique object for scientific research. In this article, we report the draft genome sequence of Janibacter limosus strain P1(28)-3 (RCAM05316) isolated from lime mud, Russia (53° 2' 0″ N, 57° 3' E). was obtained using Oxford Nanopore Technologies MinION.