A5010427957- Cervical Cancer and HPV Research
- Autophagy in Disease and Therapy
- Immune cells in cancer
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Colorectal and Anal Carcinomas
- Cancer Immunotherapy and Biomarkers
- Inflammasome and immune disorders
- Cannabis and Cannabinoid Research
- Diagnosis and treatment of tuberculosis
- Systemic Sclerosis and Related Diseases
- Vibrio bacteria research studies
- Epigenetics and DNA Methylation
- interferon and immune responses
- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
- Virus-based gene therapy research
- Cellular transport and secretion
- Immune Response and Inflammation
- Reproductive tract infections research
- Inflammatory Myopathies and Dermatomyositis
- Endometrial and Cervical Cancer Treatments
- Cancer, Stress, Anesthesia, and Immune Response
- Metastasis and carcinoma case studies
- Chemokine receptors and signaling
Université de Bourgogne
2017-2025
Inserm
2017-2025
Université de franche-comté
2011-2024
Babraham Institute
2016-2022
Université Bourgogne Franche-Comté
2016-2022
Université Paris-Saclay
2021-2022
Laboratoire d'Énergétique Moléculaire et Macroscopique, Combustion
2022
Centre de recherche Translationnelle en Médecine moléculaire
2018-2019
Centre Hospitalier Universitaire de Besançon
2014
Article9 January 2018Open Access Transparent process The WD40 domain of ATG16L1 is required for its non-canonical role in lipidation LC3 at single membranes Katherine Fletcher Signalling Programme, Babraham Institute, Cambridge, UK Search more papers by this author Rachel Ulferts Division Virology, Department Pathology, University Elise Jacquin Talitha Veith Noor Gammoh Edinburgh Cancer Research Centre, Edinburgh, Julia M Arasteh Norwich Medical School, UEA, Norwich, Ulrike Mayer School...
Non-canonical autophagy is a key cellular pathway in immunity, cancer, and neurodegeneration, characterized by conjugation of ATG8 to endolysosomal single membranes (CASM). CASM activated engulfment (endocytosis, phagocytosis), agonists (STING, TRPML1), infection (influenza), dependent on K490 the ATG16L1 WD40-domain. However, factors associated with non-canonical recruitment induction remain unknown. Here, using pharmacological inhibitors, we investigate role for V-ATPase during autophagy....
The modulation of canonical macroautophagy/autophagy for therapeutic benefit is an emerging strategy medical and pharmaceutical interest. Many drugs act to inhibit autophagic flux by targeting lysosome function, while others were developed activate the pathway. Here, we report surprising finding that many therapeutically relevant autophagy modulators with lysosomotropic ionophore properties, classified as inhibitors autophagy, are also capable activating a parallel noncanonical pathway...
While stimulator of interferon genes (STING) activation in innate immune cells the tumor microenvironment can result CD8 T cell-dependent antitumor immunity, whether STING signaling affects CD4 T-cell responses remains elusive.Here, we tested modulated effector functions vivo by analyzing tumor-infiltrating and evaluating contribution cell-derived cytokines activity ligand 2'3'-cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) two mouse models. We performed ex experiments to...
Immune checkpoint blockade (ICB) has had limited utility in several solid tumors such as breast cancer, a major cause of cancer-related mortality women. Therefore, there is considerable interest alternate strategies to promote an anti-cancer immune response. A paper co-published this issue describes how NR0B2, protein involved cholesterol homeostasis, functions within myeloid cells modulate the inflammasome and reduce expansion immune-suppressive regulatory T (T
Abstract Th17 cells can perform either regulatory or inflammatory functions depending on the cytokine microenvironment. These plastic transdifferentiate into Tregs during inflammation resolution, in allogenic heart transplantation models, cancer through mechanisms that remain poorly understood. Here, we demonstrated NLRP3 expression is essential for maintaining their immunosuppressive an inflammasome-independent mechanism. In absence of NLRP3, produce more cytokines (IFNγ, Granzyme B, TNFα)...
Previous studies have shown that DNA can be transferred from dying engineered cells to neighboring through the phagocytosis of apoptotic bodies, which leads cellular transformation. Here, we provide evidence an uptake apoptotic-derived cervical cancer by human mesenchymal cells. Interestingly, HeLa (HPV 18+) or Ca Ski (HPV16+) cells, harboring integrated high-risk HPV but not C-33 A (HPV-), were able transform recipient Human primary fibroblasts engulfed bodies effectively within 30 minutes...
Background information Efficient clearance of apoptotic cells, named efferocytosis, is a fundamental physiological process for tissue development and homeostasis. The contribution non‐professional phagocytes like fibroblasts to efferocytosis has been established, although the underlying mechanisms are not well understood. We recently demonstrated that horizontal DNA transfer can occur through uptake human papillomavirus‐positive cancer cells by primary leading their transformation. aim this...
Although survival from breast cancer has dramatically increased, many will develop recurrent, metastatic disease. Unfortunately, for this stage of disease remains very low. Activating the immune system incredible promise since it potential to be curative. However, checkpoint blockade (ICB) which works through T cells been largely disappointing cancer. One reason is a suppressive myeloid compartment that unaffected by ICB. Cholesterol metabolism and proteins involved in cholesterol...
High-risk human papillomaviruses are the etiological agents of cervical cancer and HPV16 is most oncogenic genotype. Immortalization transformation infected cells requires overexpression two viral oncoproteins E6 E7 following HPV DNA integration into host cell genome. Integration often leads to loss E2 open reading frame corresponding protein can no longer act as a transcriptional repressor on p97 promoter. Recently, it has been proposed that long control region methylation also contributes...
ABSTRACT High-risk (HR) human papillomavirus (HPV)-associated carcinogenesis is driven mainly by the overexpression of E7 and E6 oncoproteins following viral DNA integration concomitant loss E2 open reading frame (ORF). However, HR-HPV not systematically observed in cervical cancers. The protein acts as a transcription factor that governs oncogene expression. methylation CpGs E2-binding sites (E2BSs) long control region abrogates binding, thus impairing E2-mediated regulation E7/E6...
Background We have previously shown that 5-fluorouracil (5-FU) selectively kills myeloid-derived suppressor cells (MDSCs) and activates NLRP3 (NOD-leucine rich repeat pyrin containing protein 3) inflammasome. activation leads to caspase-1 production of IL-1β, which in turn favors secondary tumor growth. decided explore the effects either a heat shock (HS) or deficiency (HSP) 70, respectively inhibit increase inflammasome macrophages. Methods Caspase-1 was detected vitro MSC-2 by western blot...
The natural history of cervical cancer is closely linked to that high-risk human papillomaviruses (HPV) infection. It recognized upon HPV DNA integration, partial or complete loss the E2 open reading frame precludes expression corresponding protein, resulting in upregulation E6 and E7 viral oncoproteins. To better characterize HPV16 infection at level, load, early transcript (E2, E5, E6) were analyzed a series 158 specimens representative full spectrum disease. Overall, frequency detection...
High-risk human papillomavirus (hrHPVs), particularly HPV16 and HPV18, are the etiologic factors of ano-genital cancers some head neck squamous cell carcinomas (HNSCCs). Viral E6 E7 oncoproteins, controlled at both transcriptional post-transcriptional levels, drive hrHPVs-induced carcinogenesis. In present study, we investigated implication DEAD-box helicase eukaryotic translation initiation factor 4A3 (eIF4A3,) an Exon Junction Complex factor, in regulation gene expression. Our data...
Abstract Non-canonical autophagy is a key cellular pathway in immunity, cancer and neurodegeneration, characterised by C onjugation of A TG8 to endolysosomal S ingle- M embranes (CASM). CASM activated engulfment (endocytosis, phagocytosis), agonists (STING, TRPML1) infection (influenza), dependent on the ATG16L1 WD40-domain, specifically K490. However, factor(s) associated with non-canonical recruitment, induction, remain unknown. Here, we investigate role for V-ATPase during autophagy. We...