A5020466142- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Molecular Junctions and Nanostructures
- Quantum Dots Synthesis And Properties
- DNA and Nucleic Acid Chemistry
- Biosensors and Analytical Detection
Massachusetts Institute of Technology
2019-2024
Wireframe DNA origami assemblies can now be programmed automatically from the top-down using simple wireframe target geometries, or meshes, in 2D and 3D, either rigid, six-helix bundle (6HB) more compliant, two-helix (DX) edges. While these have numerous applications nanoscale materials fabrication due to their spatial addressability high degree of customization, no easy-to-use graphical user interface software yet exists deploy algorithmic approaches within a single, standalone interface....
Abstract Hybrid RNA:DNA origami, in which a long RNA scaffold strand folds into target nanostructure via thermal annealing with complementary DNA oligos, has only been explored to limited extent despite its unique potential for biomedical delivery of mRNA, tertiary structure characterization RNAs, and fabrication artificial ribozymes. Here, we investigate design principles three-dimensional wireframe RNA-scaffolded origami rendered as polyhedra composed dual-duplex edges. We computationally...
Abstract Control over the copy number and nanoscale positioning of quantum dots (QDs) is critical to their application functional nanomaterials design. However, multiple non-specific binding sites intrinsic surface QDs have prevented fabrication into multi-QD assemblies with programmed spatial positions. To overcome this challenge, we developed a general synthetic framework selectively attach spatially addressable on 3D wireframe DNA origami scaffolds using interfacial control QD surface....
Abstract Hybrid RNA:DNA origami, in which a long RNA scaffold strand is folded into target nanostructure via thermal annealing with complementary DNA oligos, has only been explored to limited extent despite its unique potential for biomedical delivery of mRNA, tertiary structure characterization RNAs, and fabrication artificial ribozymes. Here, we investigate design principles wireframe RNA-scaffolded origami three dimensions rendered as polyhedra composed dual-duplex edges. We...