A5084856665- Advanced biosensing and bioanalysis techniques
- Click Chemistry and Applications
- Analytical Chemistry and Sensors
- bioluminescence and chemiluminescence research
- Molecular Sensors and Ion Detection
- Advanced Biosensing Techniques and Applications
- Biotin and Related Studies
- Neuroscience and Neuropharmacology Research
- Mass Spectrometry Techniques and Applications
- RNA and protein synthesis mechanisms
- Biopolymer Synthesis and Applications
- Photoreceptor and optogenetics research
The University of Tokyo
2022-2024
Abstract Pyruvate is the end-product of glycolysis and a central metabolite involved in many biochemical pathways. However, lack high-performance (i.e., Δ F / 0 > 10) single fluorescent protein (FP)-based biosensors has hindered efforts to investigate physiological role pyruvate. Here, we present GreenPy1 ApplePy1 series, which are green FP (GFP)-based red (RFP)-based pyruvate biosensors, respectively. Both series exhibit large fluorescence intensity change (Δ ∼ 20 >40) range...
We report the development of a chemigenetic fluorescent sodium ion indicator with an affinity optimized for imaging physiologically relevant concentrations in mammalian cells.
Abstract Genetically encoded calcium (Ca 2+ ) indicators (GECIs) are widely used for imaging neuronal activity, yet current limitations of existing red fluorescent GECIs have constrained their applicability. The inherently dim fluorescence and low signal-to-noise ratio red-shifted posed significant challenges. More critically, several red-fluorescent exhibit photoswitching when exposed to blue light, thereby limiting applicability in all-optical experimental approaches. Here, we present the...