Summary. Ovaries were obtained from naturally cyclic pigs on Days 16–17, 18, 19, 20 and 21 of the oestrous cycle basis observed follicular characteristics assigned as representative early (Group 1), mid- (Groups 2 3) or late (after LH; Group 4) phase. Follicular development in gilts was compared with that ovaries prepubertal 36 5) 72 6) h after treatment 750 i.u. PMSG alone, a combination 500 hCG slaughter 30–40 later 7). After dissection all follicles >2 mm diameter, fluid volume,...

Abstract Infertility is recognized as a major problem of modern society. Assisted Reproductive Technology (ART) the one many available treatment options to cure infertility. However, efficiency ART still inadequate. Therefore, procedure’s quality constantly improving and there need determine statistical predictors well contributing factors successful treatment. There concern over application adequate analysis clinical data: should classic methods be used or would it more appropriate apply...

Abstract Principal Component Analysis is one of the data mining methods that can be used to analyze multidimensional datasets. The main objective this method a reduction number studied variables with mainte- nance as much information possible, uncovering structure data, its visualization well classification objects within space defined by newly created components. PCA very often preliminary step in preparation through creation independent components for further analysis. We first analyzing...

Abstract Infertility is currently a common problem with causes that are often unexplained, which complicates treatment. In many cases, the use of ART methods provides only possibility getting pregnant. Analysis this type data very complex. More and more often, mining or artificial intelligence techniques appropriate for solving such problems. study, classification trees were used analysis. This resulted in obtaining group patients characterized most likely to get pregnant while using vitro...

Summary. Luteal cells were obtained by digestion of luteal tissue cyclic (day 12) and early pregnant (days 12, 20 30) pigs. Suspensions the dispersed (5 × 104 ml−1) incubated for 2 h in minimum essential medium (MEM) alone (control) MEM with different concentrations prostaglandin F2α (PGF2α) PGE2 (0·01, 0·1, 1, 10, 100 1000 ng luteinizing hormone (LH) ml−1, or combinations LH + PGF2αand PGE2. Net progesterone production was measured incubation media direct radioimmunoassay. The overall...

Abstract Intrauterine insemination (IUI) is one of many treatments provided to infertility patients. Many factors such as, but not limited to, quality semen, the age a woman, and reproductive hormone levels contribute infertility. Therefore, aim our study establish statistical probability concerning prediction which groups patients have very good or poor prognosis for pregnancy after IUI insemination. For that purpose, we compare results two analyses: Cluster Analysis Kohonen Neural...

Summary Maternal effect genes (MEG) play a crucial role in early embryogenesis. In vitro culture conditions may affect MEG expression porcine oocytes and embryos. We investigated whether medium supplementation with epidermal growth factor (EGF), IL-1β or LIF (leukemia inhibitory factor) affects the mRNA level of ZAR-1 (zygote arrest 1), NPM2 (nucleoplasmin 2) DPPA3 (developmental associated protein 3) MII Cumulus–oocyte complexes (COCs) were matured NCSU-37 (control) EGF 10 ng/ml, ng/ml 50...

Concentrations of progesterone (P4), estradiol 17-beta (E2), testosterone (T) and prolactin (PRL) were estimated by RIA in porcine follicular fluid (FF) on 5th, 8th, 13th, 17th 20th day the estrous cycle 18th 28th pregnancy. The highest P4 E2 concentrations found cycle. level measured that differed (P less than 0.05) from levels 13th was different remaining days cycle, i.e. 17th. In pregnancy, these steroids much lower compared to No differences T neither nor PRL concentration at lowest 5th...

Abstract Infertility is a huge problem nowadays, not only from the medical but also social point of view. A key step to improve treatment outcomes possibility effective prediction result. In situation when phenomenon with more than 2 states needs be explained, e.g. pregnancy, miscarriage, non-pregnancy, use multinomial logistic regression good solution. The aim this paper select those features that have significant impact on achieving clinical pregnancy as well determine occurrence...

Porcine luteal cells were obtained from the corpora lutea on 13th day of estrous cycle. The digested with 0.25% trypsin and suspended in Medium 199 an addition 10% calf serum, at a concentration 5 X 10(4) cells/ml. incubated or without 4 40 mi.u./ml oxytocin, 10 100 ng/ml arginine-vasotocin, 1 microgram LH 50 U/ml hCG. Levels progesterone, testosterone estradiol 17 beta determined radioimmunological method, following 6 h incubation. It was found that progesterone secretion under influence...

Abstract There are high hopes for using the artificial neural networks (ANN) technique to predict results of infertility treatment in vitro fertilization (IVF) method. Some reports show superiority ANN approach over conventional methods. However, fully satisfactory have not yet been achieved. Hence, there is a need continue searching new data describing process, as well methods extracting information from these data. also some that use principal component analysis (PCA) before process...

Abstract Many factors play an important role in prediction of infertility treatment outcome (for example, female age and quality oocytes or embryos are the most prognostic concerning positive IVF outcome). The purpose this study was to identify a set variables that could fulfill criteria for pregnancy patients through application data mining – using discriminant analysis method. principle method is establish rules allows one place multi-dimensional objects into two analyzed groups (pregnant...

Corpora lutea were obtained from pig ovaries on Day 18 of pregnancy or pseudopregnancy.Pseudopregnancy was induced by the administration oestradiol benzoate Days 11\p=n-\15 oestrous cycle hCG 12.The luteal cells prepared for morphometric analysis and investigation steroid production in vitro dispersion with 0\m=.\25%trypsin.A blood sample each sow collected at slaughter measurement progesterone, oestradiol-17\g=b\ testosterone.The concentrations these steroids also estimated tissue medium...

Prolactin (1, 5, 10 micrograms/ml) inhibited 5 alpha-dihydrotestosterone (DHT), testosterone (T) and estradiol-17 beta (E2) secretion by luteal cells isolated from porcine corpus luteum on the 13th day of estrous cycle. The effect prolactin upon 5th 17th cycle was different. Inhibition DHT, T E2 in occurred only after 6 hours incubation, at doses micrograms/ml medium. Luteal obtained corpora lutea with considerable physiological luteolysis (17th cycle) did not react to presence incubation...

The present study was conducted to examine the effect of estradiol 17 beta (E2) and luteinizing hormone (LH) on progesterone (P4) secretion by luteal cells from early pregnant, benzoate (EB)-treated hCG-treated sows compare sensitivity these used exogenous hormones in vitro. Trypsin-dispersed (5 X 10(4) cells/ml) were incubated with E2 (doses: 0.001, 0.01, 0.1, 1 5 micrograms/ml), LH 0.1 microgram/ml) (1 microgram) plus (0.01, microgram doses). Control cultures without hormones. P4 level...

Abstract Log-linear analysis is a practical tool for examining relationships, successfully applied in many fields of science. This paper discusses the topic estimation chance getting pregnant couples that underwent ART insemination. The authors focus on finding significant interactions between variables, basis which statistical models are built. With use results log-linear analysis, model predicting chances achieving clinical pregnancy contained was Moreover, it more complete than obtained...

This study was designed to evaluate the most effective in vitro culture conditions for of pregnancy-dependent changes LH-stimulated steroidogenesis porcine luteal tissue. Mixed cells recovered from CL cyclic (Day 12) and pregnant 12 16) pigs were incubated either Eagle’s minimum essential medium (MEM) or MEM supplemented with different concentrations calf serum BSA, a defined (DM) containing 2 µg/ml insulin, 40 ng/ml hydrocortisone, 5 transferrin, 10−7 M thyroxine. Cultures treated various...

Abstract The objective of this study was to investigate whether percentage mature oocytes retrieved from ovaries stimulated with long agonist or multi-dose antagonist protocols affect the implantation, clinical pregnancy and live birth ICSI (Intracytoplasmic sperm injection) cycles. 654 cycles (long lupron) 610 flexible (antagon) were analyzed after stratification according retrieved. groups less than 30 % retrieved, both protocol statistically lower (at least p< 0.05) compared more 30% In...