A5044931798- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Gut microbiota and health
- Salmonella and Campylobacter epidemiology
- Bacillus and Francisella bacterial research
- Enzyme Production and Characterization
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
- Identification and Quantification in Food
- Microbial infections and disease research
- Microbial Metabolism and Applications
- Bacterial Genetics and Biotechnology
- Microbial Community Ecology and Physiology
- Escherichia coli research studies
- Enterobacteriaceae and Cronobacter Research
- Plant Pathogenic Bacteria Studies
- Microbial Metabolites in Food Biotechnology
- Digestive system and related health
- Legume Nitrogen Fixing Symbiosis
- Clostridium difficile and Clostridium perfringens research
- Pharmaceutical and Antibiotic Environmental Impacts
- RNA and protein synthesis mechanisms
- Bacterial biofilms and quorum sensing
Seoul National University
2012-2024
Dankook University
2019-2023
Korea Research Institute of Bioscience and Biotechnology
2021
National Institute of Animal Science
2021
Korea Atomic Energy Research Institute
2021
Kyung Hee University
2012-2020
Korea Evaluation Institute of Industrial Technology
2020
CHA University
2011-2012
University of Minnesota
2005-2011
Cargill (United States)
2006-2007
Abstract Background Bifidobacteria are frequently proposed to be associated with good intestinal health primarily because of their overriding dominance in the feces breast fed infants. However, clinical feeding studies exogenous bifidobacteria show they don't remain intestine, suggesting may lose competitive fitness when grown outside gut. Results To further understanding genetic attenuation that occurring cultures, we obtained complete genome sequence an isolate, Bifidobacterium longum...
Salmonella enterica and Escherichia coli O157:H7 are major food-borne pathogens causing serious illness. Phage SFP10, which revealed effective infection of both S. E. O157:H7, was isolated characterized. SFP10 contains a 158-kb double-stranded DNA genome belonging to the Vi01 phage-like family Myoviridae. In vitro adsorption assays showed that constant rates serovar Typhimurium were 2.50 × 10⁻⁸ ml/min 1.91 ml/min, respectively. One-step growth analysis has shorter latent period (25 min)...
Previously, a synbiotic combination of probiotic Lactobacillus gasseri 505 (LG) and new prebiotic, Cudrania tricuspidata leaf extract (CT) in fermented milk, designated FCT, showed an vitro immunomodulatory effect antioxidant activity. Although might have cancer-protective effects, these activities not been fully validated vivo. Ten-week treatment LG, CT, or FCT to azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colitis-associated colorectal cancer (CAC) mouse model reduced both the...
Background Salmonella enterica subspecies serovar Typhimurium is a Gram-negative pathogen causing salmonellosis. Typhimurium-targeting bacteriophages have been proposed as an alternative biocontrol agent to antibiotics. To further understand infection and interaction mechanisms between the host strains bacteriophages, receptor diversity of these phages needs be elucidated. Methodology/Principal Findings Twenty-five were isolated their receptors identified by screening Tn5 random mutant...
We suggest a bacteriophage genus, "Viunalikevirus", as new genus within the family Myoviridae. To date, this includes seven sequenced members: Salmonella phages ViI, SFP10 and ΦSH19; Escherichia CBA120 PhaxI; Shigella phage phiSboM-AG3; Dickeya LIMEstone1. Their shared myovirus morphology, with comparable head sizes tail dimensions, genome organization are considered distinguishing features. They appear to have conserved regulatory sequences, horizontally acquired tRNA set probable...
Escherichia coli O157:H7 and Shigella flexneri are well-known food-borne pathogens causing severe food poisoning at low infectious doses. Bacteriophages have been approved for applications by the US Food Drug Administration (FDA) suggested as natural preservatives to control specific pathogens. To develop a novel preservative against E. S. flexneri, new bacteriophage needs be isolated characterized.Bacteriophage HY01 infecting both was from swine fecal sample. belongs family Myoviridae is...
ABSTRACT A novel flagellatropic phage of Salmonella enterica serovar Typhimurium, called iEPS5, was isolated and characterized. iEPS5 has an icosahedral head a long noncontractile tail with fiber. Genome sequencing revealed double-stranded DNA 59,254 bp having 73 open reading frames (ORFs). To identify the receptor for Tn 5 transposon insertion mutants S . Typhimurium SL1344 that were resistant to isolated. All phage-resistant found have mutations in genes involved flagellar formation,...
A novel bacteriophage that infects S. aureus, SA97, was isolated and characterized. The phage SA97 belongs to the Siphoviridae family, cell wall teichoic acid (WTA) found be a host receptor of SA97. Genome analysis revealed contains 40,592 bp DNA encoding 54 predicted open reading frames (ORFs), none these genes were related virulence or drug resistance. Although few associated with lysogen formation detected in genome, produced neither nor transductant aureus. These results suggest may...
Antibiotic resistance (AR) is a complex, multifaceted global health issue that poses serious threat to livestock, humans, and the surrounding environment. It entails several elements numerous potential transmission routes vehicles contribute its development spread, making it challenging address. AR regarded as an One Health issue, has been found human, environmental components, all three domains are interconnected, opening up channels for of antibiotic resistant bacteria (ARB). turned out be...
Clostridium perfringens is a well-known pathogen that causes food-borne illnesses. Although bacteriophages can be effective natural food preservatives, phage endolysin and cell wall–binding domain (CBD) provide useful materials for lysis of C. rapid detection. The genome CPAS-15 consists 51.8-kb double-stranded circular DNA with 78 open reading frames, including an gene. apparent absence virulence factor or toxin gene suggests its safety in applications. (LysCPAS15) inhibits host cells by up...
Detecting and identifying the origins of foodborne pathogen outbreaks is a challenging. The Next-Generation Sequencing (NGS) panel method offers potential solution by enabling efficient screening identification various bacteria in one reaction. In this study, new NGS primer sets that target 18 specific virulence factor genes from six pathogens (Bacillus cereus, Yersinia enterocolitica, Staphylococcus aureus, Vibrio cholerae, parahaemolyticus, vulnificus) were developed optimized. validated...
The main cause of inflammatory bowel disease (IBD) is abnormal intestinal permeability due to the disruption tight junction barrier through a pathogen-mediated mechanism and an imbalance gut microbiota. This study aimed evaluate whether 2-ketoglutaric acid alleviated dysfunction with localization, activated transforming growth factor beta-activated kinase 1 (TAK1) inflammation pathway, regulated homeostasis microbiome in vitro vivo IBD model. Our findings revealed that significantly...
A Salmonella lytic bacteriophage, SS3e, was isolated, and its genome sequenced completely. This phage is able to lyse not only various serovars but also Escherichia coli, Shigella sonnei, Enterobacter cloacae, Serratia marcescens, indicating a broad host specificity. Genomic sequence analysis of SS3e revealed linear double-stranded DNA 40,793 bp harboring 58 open reading frames, which highly similar phages SETP13 MB78.
ABSTRACT Bifidobacterium longum DJO10A was previously demonstrated to produce a lantibiotic, but only during growth on agar media. To evaluate the feasibility of production this lantibiotic in broth media, transcription analysis lanA gene undertaken. Comparative microarray and cultures B. revealed that production, modification, transport/peptidase, immunity genes were significantly upregulated cultures, while two-component regulatory expressed equally under both conditions. This suggested...
We investigated microbiota changes following surgical colon cancer resection and evaluate effects of probiotics on recovery. This randomized double-blind trial was performed at four medical centers in South Korea. Of 68 patients expected to undergo anterior sigmoid resection, 60 were eligible, whom 29 31 received placebo, respectively, for weeks, starting one week preoperatively. Third- and/or fourth-week information syndrome (ARS), inflammatory markers, quality life obtained. Stool sample...
ABSTRACT So far, there is only fragmentary and unconfirmed information on bacteriophages infecting the genus Bifidobacterium . In this report we analyzed three prophage-like elements that are present in genomes of breve UCC 2003, longum NCC 2705, DJO10A, designated Bbr-1, Bl-1, Blj-1, respectively. These prophagelike exhibit homology with genes double-stranded DNA spanning a broad phylogenetic range host bacteria surprisingly closely related to low-G+C bacteria. All integrated tRNA Met gene,...
ABSTRACT Bifidobacterium longum DJO10A is a recent human isolate with probiotic characteristics and contains two plasmids, designated pDOJH10L pDOJH10S. The complete sequences of both these plasmids have now been determined consist circular DNA molecules 10,073 3,661 bp, G+C contents 62.2% 66.2%, respectively. Plasmid cointegrate plasmid consisting regions exhibiting very high sequence identity to other B. pNAC2 (98%) pKJ50 (96%), together another region. Interestingly, the rolling...
To understand phage infection and host cell lysis mechanisms in pathogenic Salmonella, a novel Salmonella enterica serovar Typhimurium-targeting bacteriophage, SPN9CC, belonging to the Podoviridae family was isolated characterized. The infects S. Typhimurium via O antigen of lipopolysaccharide (LPS) forms clear plaques with cloudy centers due lysogen formation. Phylogenetic analysis major capsid proteins revealed that this is member lysogen-forming P22-like group. However, comparative...