A5055354356- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Silk-based biomaterials and applications
- Electrospun Nanofibers in Biomedical Applications
- Peptidase Inhibition and Analysis
- Hydrogels: synthesis, properties, applications
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Venomous Animal Envenomation and Studies
- Biocrusts and Microbial Ecology
- Bacterial Genetics and Biotechnology
- Glycosylation and Glycoproteins Research
- Antimicrobial Peptides and Activities
- Immunotherapy and Immune Responses
- Supramolecular Self-Assembly in Materials
- CAR-T cell therapy research
- Innovative concrete reinforcement materials
- Molecular spectroscopy and chirality
- Virus-based gene therapy research
- Water resources management and optimization
- Clay minerals and soil interactions
- Therapeutic Uses of Natural Elements
- Clinical Laboratory Practices and Quality Control
- Recycled Aggregate Concrete Performance
- Bone Tissue Engineering Materials
Biotechnology Research Center
2021-2025
Alzahra University
2021-2025
Pasteur Institute of Iran
2021-2025
University of Tehran
2021-2023
Abstract In this study, the main focus was on designing and synthesizing a novel magnetic nanobiocomposite its application in hyperthermia cancer treatment. Regarding aim, sodium alginate (SA) hydrogel with CaCl 2 cross-linker formed modified by silk fibroin (SF) natural polymer halloysite nanotubes (HNTs), followed situ Fe 3 O 4 nanoparticles preparation. No important differences were detected red blood cells (RBCs) hemolysis, confirming high compatibility of treated erythrocytes...
Pseudomonas aeruginosa (PA) is a leading cause of nosocomial infections and death in cystic fibrosis patients. The study was conducted to evaluate the physicochemical structure, biological activity serum stability recombinant anti-PcrV single chain variable antibody fragment genetically attached mCH3cc domain. stereochemical properties scFv-mCH3 (YFL001) scFv (YFL002) proteins as well molecular interactions towards PcrV were evaluated computationally. subcloned fragments encoding YFL001...
Cancer is one of the leading causes mortality in world. Unfortunately, present anticancer chemotherapeutics display high cytotoxicity. Accordingly, discovery new agents with lower side effects highly necessitated. This study aimed to discover an compound from Hemiscorpius lepturus scorpion venom. Bioactivity-guided chromatography was performed isolate active against colon and breast cancer cell lines. 2D electrophoresis MALDI-TOF were identify molecule. A partial protein sequence obtained by...
Abstract The thermal electrocyclization of heptatrienyl anions is reviewed. We begin with a succinct background on the importance cycloheptane rings in complex molecules and brief survey approaches to their synthesis. also review fundamental aspects this manifold, relying Woodward–Hoffmann rules for pericyclic chemistry. Then we literature, covering burst discovery late 1960s early 1970s, more sporadic reports from 1990s present day. 1 Introduction 2 Electrocyclization Heptatrienyl Anions 3...
Overexpression of CD20 protein on the surface B cells in lymphoma can be targeted by several anti-CD20 molecules. The development accessible interactive epitopes is more favorable than full-length transmembrane affinity assessment monoclonal antibodies (mAbs).The sequence these was extracted, and effects different linker peptides location histidine (His)-tag were computationally analyzed. impact thioredoxin (Trx)-tag folding selected construct its interaction with rituximab further...
This study presents a novel approach for biological applications in the form of nanobiomagnetic system based on alginate hydrogel (SA hydrogel) specific hyperthermia cancer therapy. To enhance cell adhesion, hemocompatibility, antibacterial and anti-cancerous properties SA hydrogel, it was modified with silk fibroin (SF), natural protein. Furthermore, Fe3O4 nanoparticles were synthesized situ within network to increase magnetic nanobio composite. not only enhances dispersion structure but...
Acute myeloid leukemia (AML) is an aggressive blood cancer with frequently poor clinical outcomes. This heterogeneous malignancy encompasses genetically, molecularly, and even clinically different subgroups. makes it difficult to develop therapeutic agents that are effective for all subtypes of the disease. Therefore, a selective, universal, adaptable delivery platform capable carrying various types anti-neoplastic unmet requirement in this area. Two multifunctional fusion proteins were...
Abstract Background: Interleukin-1 receptor accessory protein (IL-1RAP) is one of the most promising therapeutic targets proposed for myeloid leukemia. Antibodies (Abs) specific to IL-1RAP could be valuable tools targeted therapy this lethal malignancy. This study about preparation a difficult-to-produce single-chain variable fragment (scFv) construct against membrane-bound isoform human using Escherichia coli ( E. ). Methods: Different approaches were examined refolding and characterization...
Pseudomonas aeruginosa is one of the opportunistic pathogens causing frequent hospital-acquired life-threatening infections in mechanically ventilated patients. The most significant virulence factor P. type III secretion system (T3SS). PcrV an important structural protein T3SS.In current investigation, a recombinant single-chain fragment variable (scFv) mAb against was expressed EnBase® (fed-batch) cultivation mode. pETiteTM N-His SUMO Kan vector, including anti-PcrV scFv gene, transformed...