A5061320681- Hepatitis C virus research
- Hepatitis B Virus Studies
- Hepatitis Viruses Studies and Epidemiology
- Liver Disease Diagnosis and Treatment
- Antenna Design and Analysis
- Polyomavirus and related diseases
- Mosquito-borne diseases and control
- RNA modifications and cancer
- MicroRNA in disease regulation
- HIV Research and Treatment
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Full-Duplex Wireless Communications
- Systemic Sclerosis and Related Diseases
- Parvovirus B19 Infection Studies
- Influenza Virus Research Studies
- Viral-associated cancers and disorders
- Viral Infections and Immunology Research
- interferon and immune responses
- RNA regulation and disease
- Immunotherapy and Immune Responses
Gilead Sciences (United States)
2022-2024
University of British Columbia
2017-2023
Hôpital de la Croix-Rousse
2023
Hospices Civils de Lyon
2023
Novartis (United States)
2019-2020
Novartis Institutes for BioMedical Research
2019
Novartis (France)
2019
Novartis (Switzerland)
2019
A hallmark of chronic hepatitis B (CHB) virus infection is the presence high circulating levels non-infectious small lipid HBV surface antigen (HBsAg) vesicles. Although rare, sustained HBsAg loss idealized endpoint any CHB therapy. molecule, RG7834, has been previously reported to inhibit expression by targeting terminal nucleotidyltransferase proteins 4A and 4B (TENT4A TENT4B). In this study, we describe a genome-wide CRISPR screen identify other potential host factors required for gain...
In patients with chronic hepatitis C virus (HCV) infection, viral hijacking of the host-cell biosynthetic pathways is associated altered lipid metabolism, which contributes to disease progression and may influence antiviral response. We investigated molecular interplay among four key regulators homeostasis [microRNA (miR)-122, miR-24, miR-223, proprotein convertase subtilisin/kexin type 9 (PCSK9)] in HCV-infected (n=72) who achieved a treatment-based cure after interferon-based therapy...
Viral hijacking and manipulation of host-cell biosynthetic pathways by human enveloped viruses are shared molecular events essential for the viral lifecycle. For Flaviviridae members such as hepatitis C virus dengue (DENV), one key subsets cellular that undergo is lipid metabolic pathways, underlining importance lipids and, in particular, droplets (LDs) infection. Here, we hypothesize targeting enzymes act regulators homeostasis LD formation could represent a powerful approach to developing...
Chronic hepatitis B virus (HBV) infection is characterized by the presence of high circulating levels non-infectious lipoprotein-like HBV surface antigen (HBsAg) particles thought to contribute chronic immune dysfunction in patients. Lipid and metabolomic analysis humanized livers from immunodeficient chimeric mice (uPA/SCID) revealed that dysregulates several lipid metabolic pathways. Small molecule inhibitors biosynthetic pathway enzymes acetyl-CoA carboxylase (ACC), fatty acid synthase,...
The standard of care for the treatment chronic hepatitis B (CHB) is typically lifelong with nucleos(t)ide analogs (NAs), which suppress viral replication and provide long-term clinical benefits. However, infectious virus can still be detected in patients who are virally suppressed on NA therapy, may contribute to failure these agents cure most CHB patients. Accordingly, new antiviral options being developed enhance suppression (HBV) combination NAs ("antiviral intensification"). Here, we...
Hepatitis E virus (HEV) is an emerging found predominately in developing countries; it causes estimated 20 million infections, which result approximately 57,000 deaths a year. Although known that the nonstructural proteins of HEV ORF1 are expressed as single transcript, there debate to whether functions polyprotein or if processed into separate domains via viral endogenous cellular protease. Here we present first structural and biophysical characterization protein using construct has...
Capsid assembly mediated by hepatitis B virus (HBV) core protein (HBc) is an essential part of the HBV replication cycle, which target for different classes capsid modulators (CAMs). While both CAM-A ("aberrant") and CAM-E ("empty") disrupt nucleocapsid reduce extracellular DNA, CAM-As can also surface antigen (HBsAg) triggering apoptosis HBV-infected cells in preclinical mouse models. However, there have not been substantial HBsAg declines chronic (CHB) patients treated with to date. To...
In pursuit of therapeutics for human polyomaviruses, we identified a peptide derived from the BK polyomavirus (BKV) minor structural proteins VP2/3 that is potent inhibitor BKV infection with no observable cellular toxicity. The thirteen-residue binds to major protein VP1 single-digit nanomolar affinity. Alanine-scanning three key residues, substitution each which results in ~1000 fold loss binding affinity concomitant reduction antiviral activity. Structural studies demonstrate specific...
The role of hepatocytes in producing chemokines and triggering liver inflammation damage chronic hepatitis D (CHD) is not fully understood. Herein, we investigated the contribution primary human (PHHs) infected with HDV by CXCL9-11. We performed quantitative PCR, RNA situ hybridisation, activation-induced marker (AIM) assays, FACS analysis to investigate CXCR3/CXCL9-11 receptor/ligand axis T cells peripheral blood livers from patients B (n = 27 18, respectively) CHD 20 respectively)....
Hepatitis C virus (HCV) hijacks host lipid metabolic pathways as part of its replication cycle. Chronic HCV infection is associated with altered metabolism, which both contributes to disease progression and influences response therapy. To help understand how important chronic liver disease, we investigated the molecular interplay between four circulating regulators homeostasis (miR-122, miR-24, miR-223 proprotein convertase subtilisin/kexin type 9 (PCSK9)) in HCV-infected patients who...
Abstract Hepatitis E virus (HEV) is a 7.2 kb positive-sense, single-stranded RNA containing three partially overlapping reading frames, ORF 1-3. All non-structural proteins required for viral replication are encoded by ORF1 and transcribed as single transcript. Computational analysis of the complete polyprotein identified previously uncharacterized region predicted secondary structure bordered two disordered regions coinciding with putative cysteine protease. Following successful cloning,...
GRAPHICAL ABSTRACT SUMMARY A hallmark of chronic hepatitis B virus (CHB) infection is the presence high circulating levels non-infectious small lipid HBV surface antigen (HBsAg) vesicles. Although rare, sustained HBsAg loss idealized endpoint any CHB therapy. novel molecule RG7834 has been previously reported to inhibit expression by targeting terminal nucleotidyltransferase protein 4A and 4B (TENT4A TENT4B). In this study, we describe a genome-wide CRISPR screen identify other potential...