A5063745322- Cannabis and Cannabinoid Research
- Neuroscience and Neuropharmacology Research
- Sleep and Wakefulness Research
- Neuroscience of respiration and sleep
- Memory and Neural Mechanisms
- Neurotransmitter Receptor Influence on Behavior
- Biochemical Analysis and Sensing Techniques
- Diet, Metabolism, and Disease
- Alcohol Consumption and Health Effects
- Lipid metabolism and biosynthesis
- Stress Responses and Cortisol
- Pancreatic function and diabetes
- Forensic Toxicology and Drug Analysis
- Inflammatory mediators and NSAID effects
- Phosphodiesterase function and regulation
- Metabolomics and Mass Spectrometry Studies
- Olfactory and Sensory Function Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Eicosanoids and Hypertension Pharmacology
- Pain Mechanisms and Treatments
- Memory Processes and Influences
- GABA and Rice Research
- Cholinesterase and Neurodegenerative Diseases
- Essential Oils and Antimicrobial Activity
- Neuroendocrine regulation and behavior
National Cheng Kung University
2013-2024
Indiana University Bloomington
2006-2013
Background and purpose: Recombinant cyclooxygenase‐2 (COX‐2) oxygenates 2‐arachidonoylglycerol (2‐AG) in vitro . We examined whether prostaglandin E 2 glycerol ester (PGE ‐G), a COX‐2 metabolite of 2‐AG, occurs endogenously affects nociception immune responses. Experimental approach: Using mass spectrometric techniques, we PGE ‐G vivo if its levels are altered by inhibition COX‐2, monoacylglycerol (MAG) lipase or inflammation induced carrageenan. also the effects on rats NFκB activity...
N-arachidonoyl glycine (NAGly) is an endogenous signaling lipid with a wide variety of biological activity whose biosynthesis poorly understood. Two primary biosynthetic pathways have been proposed. One suggests that NAGly formed via enzymatically regulated conjugation arachidonic acid (AA) and glycine. The other oxidative metabolite the cannabinoid, anandamide (AEA), through alcohol dehydrogenase. Here using both in vitro vivo assays measuring metabolites LC/MS/MS we test hypothesis are...
<i>N</i>-arachidonoyl glycine is an endogenous arachidonoyl amide that activates the orphan G protein-coupled receptor (GPCR) GPR18 in a pertussis toxin (PTX)-sensitive manner and produces antinociceptive antiinflammatory effects. It produced by direct conjugation of arachidonic acid to oxidative metabolism endocannabinoid anandamide. Based on presence enzymes conjugate fatty acids with high abundance palmitic brain, we hypothesized formation saturated <i>N</i>-acyl <i>N</i>-palmitoyl...
Cannabinoid receptors and their ligands constitute an endogenous signaling system that is found throughout the body, including eye. This can be activated by Delta(9)-tetrahydrocannabinol, a major drug of abuse. Cannabinoids offer considerable therapeutic potential in modulating ocular immune inflammatory responses regulating intraocular pressure. The location cannabinoid receptor 1 (CB(1)) retina known, but recently constellation proteins has been identified produce break down...
Background and Purpose GPR 18 is a recently deorphaned lipid receptor that activated by the endogenous N ‐arachidonoyl glycine ( NAG ly) as well behaviourally inactive atypical cannabinoid, abnormal cannabidiol A bn‐ CBD ). The presence and/or function of any 18‐based ocular signalling system remain essentially unstudied. objectives this research are: (i) to determine disposition receptors ligands in anterior murine eye, (ii) examine effect activation on intraocular pressure IOP ) model,...
Endocannabinoid, particularly 2-arachidonoyl glycerol (2-AG), signaling has recently emerged as a molecular determinant of neuronal migration and synapse formation during cortical development. However, the cell type specificity regulation spatially temporally confined morphogenic 2-AG signals remain unexplored. Here, we demonstrate that genetic pharmacological manipulation CB 1 cannabinoid receptors permanently alters cholinergic projection neuron identity hippocampal innervation. We show...
Cannabinoids, such as Δ9-THC, act through an endogenous signaling system in the vertebrate eye that reduces IOP via CB1 receptors. Endogenous cannabinoid (eCB) ligand, 2-arachidonoyl glycerol (2-AG), likewise activates and is metabolized by monoacylglycerol lipase (MAGL). We investigated ocular 2-AG its regulation MAGL therapeutic potential of harnessing eCBs to lower IOP.We tested effect topical application blockers normotensive mice examined changes eCB-related lipid species eyes spinal...
Purpose: GPR119 is a G protein–coupled receptor that may be the endogenous target for 2-oleoylglycerol (2-OG), lipid related to endocannabinoid family of neuromodulators. Interest in has centered on its role regulating insulin secretion; however, not been examined eye. The purpose this study was explore potential GPR119-based signaling system murine Methods: We used combination RT-PCR, immunohistochemistry, measurement, and IOP measurement normotensive mouse model, with knockout mice as...
Cannabinoid CB1 receptors are found in abundance the vertebrate eye, with most tissue types expressing this receptor. However, function of corneal epithelial cells (CECs) is poorly understood. Interestingly, corneas knockout mice heal more slowly after injury via a mechanism proposed to involve protein kinase B (Akt) activation, chemokinesis, and cell proliferation. The current study examined role cannabinoids CEC migration greater detail.We determined healing. We consequences their...
We previously showed that cannabinoid-related GPR18 receptors are present in the murine corneal epithelium, but their function remains unknown. The related CB1 regulate healing, possibly via chemotaxis. therefore examined a potential role for epithelial chemotaxis and wound healing.
The endocannabinoid (eCB) signaling system has been functionally implicated in many brain regions. Our understanding of the role cannabinoid receptor type 1 (CB ) olfactory processing remains limited. Cannabinoid is involved regulating glomerular activity main bulb (MOB). However, cannabinoid-related circuitry inputs to mitral cells MOB not fully determined. Using anatomical and functional approaches we have explored this question. CB was present periglomerular processes a GAD65-positive...
Rimonabant is well recognized as a cannabinoid CB_1 receptor antagonist/inverse agonist. not only antagonizes the effects induced by exogenous cannabinoids and endocannabinoids at receptors, it also exerts several pharmacological behavioral independent of inactivation. For example, rimonabant can function low-potency mixed agonist/antagonist transient potential vanilloid 1 (TRPV1). Hence, important to explain underlying mechanisms diverse physiological with caution. Interestingly, has...
As a noninvasive behavioral intervention, the retrieval-extinction (R-E) procedure has drawn much research attention for its capacity to target reconsolidation of maladaptive memories. However, later findings suggest that cause and consequence R-E may be more complicated than previously suggested. For example, could increase an animal's motivation drug-seeking under certain circumstances, reversed extinction-retrieval (E-R) also suppress drug memory. Two possible mechanisms underlying have...