A5038013851- Drug Transport and Resistance Mechanisms
- Pharmacogenetics and Drug Metabolism
- Estrogen and related hormone effects
- Nitric Oxide and Endothelin Effects
- Eicosanoids and Hypertension Pharmacology
- Amino Acid Enzymes and Metabolism
- Neurological Disorders and Treatments
- Biochemical effects in animals
- Trace Elements in Health
- DNA and Nucleic Acid Chemistry
- Pharmacological Effects and Toxicity Studies
- Hormonal Regulation and Hypertension
- Human Health and Disease
- Redox biology and oxidative stress
- Free Radicals and Antioxidants
- Genomics, phytochemicals, and oxidative stress
- Neuroscience and Neuropharmacology Research
- Adipose Tissue and Metabolism
- Genetic Neurodegenerative Diseases
- Cholesterol and Lipid Metabolism
- Neuroendocrine regulation and behavior
- Blood Pressure and Hypertension Studies
- Cancer, Hypoxia, and Metabolism
- Biotin and Related Studies
- Alcohol Consumption and Health Effects
Ryazan State Medical University named after Academician I.P. Pavlov
2014-2025
P-glycoprotein (Pgp, ABCB1, MDR1) is an efflux transporter protein that removes molecules from the cells (outflow) into extracellular space. Pgp plays important role in pharmacokinetics, ensuring absorption, distribution, and excretion of drugs its substrates, as well transport endogenous (steroid thyroid hormones). It also contributes to tumor cell resistance chemotherapy. In this review, we summarize mechanisms regulation during oxidative stress. The currently available data suggest has a...
The effect of the nitric oxide donor S-nitrosoglutathione on level and activity organic anion transporting polypeptide 1B1 (OATP1B1), as well expression SLCO1B1 gene encoding transporter protein, was investigated in HepG2 cells. study has shown that treatment cells with for 3 h did not influence content OATP1B1. Incubation (10-500 μM) 24 increased expression, OATP1B1, protein. Induction OATP1B1 protein by NO suppressed soluble guanylate cyclase (sGC) inhibitor, 10 μM ODQ...
To study the effect of Mexidol on level factors regulating neuroregenesis. The was performed male Wistar rats. Focal cerebral ischemia reproduced by endovascular occlusion-reperfusion right medial artery. duration occlusion 60 min. During beginning reperfusion, animals were given a single intravenous injection saline solution or drug at dose 50 mg/kg. 4, 8 and 24 hours after reperfusion in ischemic hemisphere, relative amount molecules neurogenesis estimated Western blot method....
S-nitrosoglutathione (GSNO) is the S-nitrosated derivative of glutathione (GSH). GSNO an endogenous class NO donors and a natural depot in biological systems. Organic anion transporting polypeptide 1B1 (OATP1B1) influx transporter that expressed liver. OATP1B1 plays important role transport exogenous substances. Various pathways for regulation have been described. In present study, involvement by was examined. For HEK293-OATP1B1, it has shown not substrate OATP1B1, but can participate GSH...
Objective. To study the effect of active metabolites bioflavonoids (hesperetin and diosmetin) on remodeling markers (von Willebrand factor (vWF), platelet-endothelial cell adhesion molecule-1 (CD31), fibronectin (FN), vimentin (VIM), plasminogen activator inhibitor type 1 (PAI-1)) in primary culture endothelial cells. Material methods. The was conducted cultures human umbilical vein cells (HUVEC) vitro. We selected concentrations substances considering literature data, serum content after...
Background. Glycoprotein-P (Pgp, АВСВ1) is a transporter protein participating in pharmacokinetics of medical drugs, and also development resistance tumor cells to chemotherapy.
 Aim. To study the influence progesterone on activity Pgp vitro cell model human small intestinal epithelium.
 Materials Methods. The work was conducted Caco-2 cells. evaluated by transport fexofenadine special transwell-system. Concentration analyzed HPLC method. amount determined EIA Four series...
Cells of the Caco-2 line have basic properties enterocytes small intestine, and therefore can be used to study absorption medicinal substances. Aim. To characterize cell from Institute Cytology Russian Academy Sciences evaluate with its help mechanism original domestic drug - ethylmethylhydroxypyridine succinate (EMGPS). Materials methods. The was performed on Caco2 cells that were cultured for 21 days, since at this time their spontaneous differentiation into polarized similar intestine...
BACKGROUND: Alcohol (ethanol) use disorder is a destructive condition with alterations in impaired energy and metabolic processes activation of free radical oxidation processes. Therefore, the antioxidants complex therapy this pathology pathogenetically justified, one which original domestic drug ethylmethylhydroxypyridine succinate.
 AIM: To examine effectiveness succinate intravenous, intramuscular, intragastric methods administration to male ICR mice modeling alcohol...
Цель: проанализировать зависимость состояния лизосомального цистеинового протеолиза от степени окислительной модификации белков в условиях эксперимен тального моделирования ишемии и ишемии-реперфузии.Исследование выполнено на лабораторных животных -крысах линии Wistar.Созданы две экспериментальные модели -ишемии реперфузии путем пережатия брюшного отдела аорты (первая группа) с последующим кондиционированием (вторая группа).Активность лизосомальных протеиназ уровень окислительно-модифи...
INTRODUCTION: Nuclear factor E2-related 2 (Nrf2) is a member of capncollar (CNC) family subfamily leucine zipper transcription factors that regulates cell protection against toxic substances and oxidants.
 AIM: To determine location, mechanism activation role Nrf2 in conditions oxidative stress vitro.
 MATERIALS AND METHODS: The study was performed on human colon adenocarcinoma line (Caco-2). Oxidative (OS) modeled by adding hydrogen peroxide (Н2О2) at concentrations 0.1 М100 М to...
Актуальность.Гликопротеин-Р (Pgp, АВСВ1) -белок-транспортер, обеспечивающий защиту органов и тканей от ксенобиотиков, являющихся его субстратами, выводя их из клеток во внеклеточное пространство биологические жидкости.Цель.Изучить влияние эстрадиола на активность Pgp in vitro линии Caco-2.Материалы методы.Исследования выполнены Caco-2.Активность анализировали по транспорту маркерного субстрата -фексофенадина в трансвеллсистеме.Концентрацию фексофенадина оценивали методом высокоэффективной...
INTRODUCTION: C2C12 is an immortalized mouse myoblast cell line actively used as in vitro experimental models to study skeletal muscle metabolism biomedical research. cells can differentiate into myocytes under appropriate culture conditions. It important understand the specifics of each stage myogenesis and its regulatory mechanisms for targeted exposure development medical drugs, taking account pathogenesis various diseases.
 AIM: To describe method culturing С2С12 myogenesis.
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We investigated the mechanisms of P-glycoprotein (P-gp) transporter regulation in Caco-2 cells under exogenous and endogenous oxidative stress (OS). Exogenous OS was modeled by exposure growth medium to hydrogen peroxide at concentrations 0.1, 0.5, 1 μM for 24 h or 10 72 h. Endogenous incubating with DL-buthionine sulfoximine (BSO, gamma-glutamylcysteine synthetase inhibitor) a concentration 10, 50, 100 The levels intracellular reactive oxygen species (ROS) were assessed using MitoTracker...
Aim. To study the influence of substrate for nitrogen oxide (II) synthesis - L-arginine and non-selective NO-synthase inhibitor N-nitro-L-arginine-methyl ester on protein oxidative modification in combination with rats’ thymocytes cathepsin H activity estimation vitro. Methods. The was performed male Wistar rats body weight 280-320 g. Freshly-separated were incubated vitro full nutrient medium containing 5 мМ (n=8) or 24 hours at temperature 37 °C (n=8). Control group consisted same...
Pregnan X receptor (PXR) is a nuclear that plays an important role in the regulation of expression biotransformation and metabolic enzymes. The functioning possible mechanisms PXR under conditions nitrosative stress have not been studied, which served as purpose this study. work was performed on Caco-2 cells. Nitrosative (NS) modeled using S-nitrosoglutathione (GSNO) at concentrations 1 μM, 10 50 100 500 μM incubation during 3 h, 24 72 h. amount assessed byWestern blotting. Incubation cells...
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