Porcine circovirus (PCV) was initially recognized as a contaminant of continuous pig kidney cell lines and not thought to be pathogenic. Antibodies reactive the culture isolate PCV (PCV PK-15) are prevalent in swine population worldwide. Recently, PK-15-like antigen nucleic acid were demonstrated lesions associated with wasting syndromes pigs North America Europe. Monoclonal antibodies raised circo-viruses isolated from highlighted differences between these circoviruses PK-15 isolate. This...

Chicken anemia virus (CAV) causes cytopathogenic effects in chicken thymocytes and cultured transformed mononuclear cells via apoptosis. Early after infection of cells, the CAV-encoded protein VP3 exhibits a finely granular distribution within nucleus. At later stage infection, forms aggregates. this point, cell becomes apoptotic cellular DNA is fragmented condensed. By immunogold electron microscopy was shown to be associated with structures. In vitro, expression induced apoptosis...

The complete nucleotide sequence (1759 nt) of the ssDNA genome porcine circovirus (PCV) was determined from a cloned dsDNA replicative form isolated PCV-infected cells. Sequence analysis detected no significant nucleic acid or protein similarity with another animal circovirus, chicken anaemia virus (CAV) but, surprisingly, highest obtained between product largest predicted PCV ORF (ORF1; encoding potential 35·7 kDa) and putative encoded by plant banana bunchy top (BBTV). High also other...

Chicken anaemia agent (CAA) was purified using differential centrifugation and successive cycles of equilibrium density gradient sucrose CsCl. The purification method dependent on the use an antigen-detecting ELISA based a CAA-specific monoclonal antibody. Virus particles banded at 1.33 to 1.34 g/ml in CsCl measured 23.5 +/- 0.8 nm diameter. Purified preparations contained one major polypeptide (Mr 50,000) single-stranded, circular DNA (2.3 kb). CAA shares some biochemical characteristics...

ABSTRACT Cell culture isolates of salmon pancreas disease virus (SPDV) farmed Atlantic and sleeping (SDV) rainbow trout were compared. Excluding the poly(A) tracts, genomic nucleotide sequences SPDV SDV RNAs include 11,919 11,900 nucleotides, respectively. Phylogenetic analysis places between New World viruses Venezuelan equine encephalitis Eastern Old Aura Sindbis virus. When compared to each other, show 91.1% sequence identity over their complete genomes, with 95 93.6% amino acid...

The circular, single-stranded DNA genome of a novel circovirus canaries, tentatively named canary (CaCV), was cloned and sequenced. Sequence analysis indicated that the 1952 nucleotides (nt) in size had potential to encode three viral proteins, including putative capsid replication-associated (Rep) proteins. CaCV shared greatest sequence similarity (58.3% nt identity) with newly characterized columbid (CoCV) more distantly related two porcine strains, PCV1 PCV2, beak feather disease virus...

Earlier work identified and biologically characterized antigenically distinct enterovirus-like viruses (ELVs) of chickens. Three these ELVs can now be as astroviruses. Characterization involved the use a hitherto undescribed, degenerate primer-based reverse transcription-polymerase chain reaction (RT-PCR) to amplify astrovirus open reading frame (ORF) 1b-specific cDNA fragments followed by nucleotide sequence determination analysis amplified fragments. ELV-1 was confirmed an isolate avian...

SUMMARY A virus, designated 132 was isolated from the faeces of chickens in chick embryo liver cell cultures. The morphology and morphogenesis virus were indistinguishable that rotaviruses. nucleic acid had nuclease resistance double-stranded RNA, separated by polyacrylamide gel electrophoresis into 11 segments with mol. wt. ranging 2.07 × 106 to 0.20 106. SPF susceptible oral infection which replicated villous epithelial cells small intestine. therefore a rotavirus morphological,...

Circoviruses are small, nonenveloped icosahedral animal viruses characterized by circular single-stranded DNA genomes. Their genomes the smallest possessed viruses. Infections with circoviruses, which can lead to economically important diseases, frequently result in virus-induced damage lymphoid tissue and immunosuppression. Within family Circoviridae, different genera distinguished differences genomic organization. Thus, Chicken anemia virus is genus Gyrovirus, while porcine circoviruses...

Some important biological and physico-chemical characteristics of porcine circovirus are reported. These include a study on the host distribution in nature, levels colostrum-derived antibodies piglets from sero-positive sows susceptibility range cell cultures to infection with this virus. The results haemagglutination studies, resistance pH 3, chloroform heat also reported as comparative buoyant densities sedimentation coefficients chicken anaemia

To evaluate the economic effects of subclinical chicken anemia agent (CAA) infection on broiler performance, clinically normal flocks were grouped into two categories: A) in which none 10 birds sampled at slaughter had antibody to CAA, and B) six or more similarly CAA antibody. Production performance parameters 25 each category compared. No statistically significant differences found between A B major production such as sex, feed manufacturer, type litter, stocking density, age slaughter....

Six isolates of rotavirus were made from turkeys and two chickens. Three these required trypsin treatment for isolation serial passage in cell cultures. The remainder isolated without treatment. Most viruses chick embryo liver cultures the faeces birds aged under 1 week. In six eight instances, was associated with enteric disturbance, characterised by signs such as diarrhoea, poor or abnormal appetite, abnormally fluid gaseous intestinal contents increased mortality. Cross immunofluorescence...

Detection systems for chicken anemia virus (CAV) antigen in paraffin sections were evaluated. Mouse monoclonal antibodies to CAV used conjunction with an avidin-biotin-peroxidase-complex detection system gave best results. Immunoreactivity of was markedly affected by fixation. Fixation neutral buffered formalin 6 hours Use decalcifying fluid containing formic acid eliminated immunoreactivity CAV, whereas use EDTA solution did not. In a sequential study, and lesions first detected bone...

Complementary oligonucleotide primers which flank a 675-bp DNA fragment encompassing part of the putative gene for capsid protein chicken anemia virus (CAV) were used enzymatic amplification CAV by polymerase chain reaction (PCR). Application dot blot hybridization assay using 32P-labeled cloned probe allowed PCR product amplified from as little 0.1 fg target sequence to be detected. When it was amplification, extracted thymus tissue guanidine isothiocyanate-based method proved more...

The purpose of this study was to molecularly characterize circoviruses that infect finches and gulls. Circovirus-specific DNAs were isolated using polymerase chain reaction methods from bursa Fabricius tissues a Gouldian finch (Chloebia gouldiae) herring gull (Larus argentatus) known be circovirus-infected. Nucleotide sequence determination analysis cloned genomic showed these represented novel members the genus Circovirus family Circoviridae, have been tentatively named Finch circovirus...

A total of 151 specimens turkey and chicken faeces intestinal contents were examined for the presence viruses by electron microscopy. Viruses detected in 48 these (32%). The most frequently observed rotaviruses enterovirus-like particles. Rotavirus infection was associated with outbreaks diarrhoea turkeys, but symptomless rotavirus seen broiler chickens. Adeno-viruses infectious bursal disease virus also faeces. best method preparing faecal material examination employed initial purification...