A5070489863- Animal health and immunology
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Gastrointestinal disorders and treatments
- Veterinary Oncology Research
- Respiratory viral infections research
- Microbial infections and disease research
- Animal Virus Infections Studies
- Reproductive Physiology in Livestock
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Biotin and Related Studies
- Herpesvirus Infections and Treatments
- T-cell and Retrovirus Studies
- Monoclonal and Polyclonal Antibodies Research
- Influenza Virus Research Studies
- Veterinary Equine Medical Research
- Virology and Viral Diseases
- Infant Nutrition and Health
- Toxoplasma gondii Research Studies
- Viral Infections and Immunology Research
- Thyroid Disorders and Treatments
- Sarcoma Diagnosis and Treatment
- Parasitic Infections and Diagnostics
- Immune Response and Inflammation
University of Tennessee at Knoxville
2024
University of Saskatchewan
2011-2023
Saskatchewan Disease Control Laboratory
2020-2022
Auburn University
2014-2019
Kansas State University
2019
University of Guelph
2015
Agriculture and Agri-Food Canada
2015
Western University of Health Sciences
1989-2014
Diagnostic Services Manitoba
2005
University of Nebraska–Lincoln
2002
Samples of lung, liver, kidney, pancreas, spleen, and lymph node from pigs with postweaning multisystemic wasting syndrome California (USA) samples mesenteric nodes similarly diseased Brittany (France) were examined by light microscopy, in situ hybridization (ISH), and/or virus isolation. Whole genomic probes for porcine circovirus (PCV) chicken anemia (CAV) used ISH. Tissue homogenate supernatants inoculated onto PK/15 cells isolation, the presence viral antigen particles was verified...
Porcine circovirus (PCV) was initially recognized as a contaminant of continuous pig kidney cell lines and not thought to be pathogenic. Antibodies reactive the culture isolate PCV (PCV PK-15) are prevalent in swine population worldwide. Recently, PK-15-like antigen nucleic acid were demonstrated lesions associated with wasting syndromes pigs North America Europe. Monoclonal antibodies raised circo-viruses isolated from highlighted differences between these circoviruses PK-15 isolate. This...
Neonatal gnotobiotic piglets were inoculated with tissue homogenates and low- high-passage cell culture material to determine if the lesions of newly described porcine postweaning multi-systemic wasting syndrome (PMWS) could be reproduced. For this, 17 3-day-old intranasally pelleted chloroform-treated, filtered extracts from cultures, filter-sterilized lymphoid PMWS-affected piglets, or control materials. Piglets maintained in germ-free isolators for up 5 weeks after infection prior...
Porcine circovirus 2 (PCV2) is a recently identified agent that has been associated with post-weaning multisystemic wasting syndrome (PMWS) in swine populations. In this report, the potential spectrum of disease PCV2 expanded by evidence vertical transmission and reproductive failure. was isolated from litter aborted piglets farm experiencing late-term abortions stillbirths. Severe, diffuse myocarditis present 1 piglet extensive immunohistochemical staining for antigen. Variable amounts...
Postweaning multisystemic wasting syndrome (PMWS) is an emerging disease in swine. Recently, the has been reproduced with inocula containing a newly described porcine circovirus (PCV), designated PCV 2, and parvovirus (PPV). In order to determine if these viruses interact naturally acquired PMWS, affected tissues from field cases were examined by immunohistochemistry (IHC) polymerase chain reaction (PCR) for 2 PPV, as well PCR other recognized circovirus, 1. Porcine was detected or IHC fixed...
Thirty-two monoclonal antibodies directed against epitopes on bovine viral diarrhea virus proteins and glycoproteins were tested for immunohistochemical reactivity with in formalin-fixed paraffin-embedded tissues from 45 cases of virus-associated mucosal disease. Only one antibody, designated 15C5, which reacts the 48-kD glycoprotein virus, detected an epitope preserved these specimens. Monoclonal antibody 15C5 a polyclonal to successfully antigens 44/45 disease did not react 30 uninfected...
This study evaluated how feeding colostrum- or a colostrum-milk mixture for 3 d postnatal affects plasma glucagon-like peptide-2 (GLP-2), serum insulin-like growth factor-1 (IGF-1), and small intestinal histomorphology in calves. Holstein bulls (n = 24) were fed colostrum at 2 h randomly assigned to receive either (COL), whole milk (WM), 1:1 COL:WM (MIX) every 12 from 72 h. A jugular venous catheter was placed 1 sample blood frequently the duration of experiment. Samples collected 1, 2, 3,...
The objective of this study was to evaluate the efficacy immunohistochemical (IHC) staining for diagnosis persistent bovine viral diarrhea virus (BVDV) infection using formalin-fixed, paraffin-embedded skin biopsy specimens. Skin from 41 42 calves shown be persistently infected (PI) with BVDV by repeated isolation more than 3 weeks apart were immunohistochemically positive antigen. Positive IHC most pronounced in keratinocytes and hair follicle epithelium, matrix cells bulb, dermal papilla....
Inclusion bodies with staining affinity and ultrastructural characteristics typical of circoviruses that stained positive for porcine circovirus (PCV)-like virus were demonstrated in association granulomatous lesions multiple tissues three clinically ill 10- to 12-week-old pigs. A syndrome poor growth wasting 5–15% weaned pigs was an intermittent problem on a 450-sow one-site farrow-to-finish swine farm Indiana. Routine diagnostic testing did not demonstrate cause. Gross examination...
A positive score on a modified acid-fast (MAF)-stained smear test of fresh placenta was used to identify group bovine abortion submissions believed be infected with Coxiella burnetii. Immunohistochemical (IHC) testing for and Chlamydia antigens performed 14 MAF smear-positive cases as well 29 smear-negative received during the study period. Nine 1 case were Coxiella-positive via IHC test. No placentas antigen. Various histopathologic features categorized all presence or absence selected risk...