A5076485985- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- Malaria Research and Control
- Insect and Arachnid Ecology and Behavior
- Insect symbiosis and bacterial influences
- Animal Behavior and Reproduction
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Plant and animal studies
- Insect Resistance and Genetics
- Vector-borne infectious diseases
Imperial College London
2017-2024
Manipulation of the mosquito gut microbiota can lay foundations for novel methods disease transmission control. Mosquito blood feeding triggers a significant, transient increase microbiota, but little is known about mechanisms by which controls this bacterial growth whilst limiting inflammation epithelium. Here, we investigate epithelial response to changing load upon in malaria vector Anopheles coluzzii. We show that synthesis and integrity peritrophic matrix, physically separates...
Malaria parasites develop as oocysts in the mosquito for several days before they are able to infect a human host. During this time, mosquitoes take bloodmeals replenish their nutrient and energy reserves needed flight reproduction. We hypothesized that these critical oocyst growth experimental infection protocols, typically involving single bloodmeal at time of infection, cause nutritional stress developing oocysts. Therefore, enumerating disregarding differentiation state may lead...
After being ingested by a female Anopheles mosquito during bloodmeal on an infected host, and before they can reach the salivary glands to be transmitted new Plasmodium parasites must establish infection of midgut in form oocysts. To achieve this, first survive series robust innate immune responses that take place prior to, during, immediately after ookinete traversal epithelium. Understanding how may evade these could highlight ways block malaria transmission. We show sporozoite surface...
The yellow fever mosquito, Aedes aegypti, mates in flight as part of ephemeral aggregations termed swarms. Swarms contain many more males than females, and are thought to be subject intense sexual selection.1Yuval B. Mating systems blood-feeding flies.Annu. Rev. Entomol. 2006; 51: 413-440https://doi.org/10.1146/annurev.ento.51.110104.151058Crossref PubMed Scopus (133) Google Scholar,2Cator L.J. Wyer C.A.S. Harrington L.C. Mosquito selection reproductive control programs.Trends Parasitol....
Gene drives for mosquito population modification are novel tools malaria control. Strategies to safely test antimalarial effectors in the field required. Here, we modified Anopheles gambiae zpg locus host a CRISPR/Cas9 integral gene drive allele ( D ) and characterized its behaviour resistance profile. We found that dominantly sterilizes females but can induce efficient at other loci when it itself encounters resistance. combined with multiple previously non-autonomous payload that, as...
Abstract Malaria parasites develop and grow as oocysts in the mosquito for several days before being able to infect another human. During this time, mosquitoes take regular bloodmeals replenish their nutrient energy reserves needed flight reproduction. We hypothesized that supplemental are critical oocyst growth experimental infection protocols, typically involving a single bloodmeal, cause nutritional stress developing oocysts. Therefore, enumerating independently of differentiation state...
mosquitoes are vectors of several viruses major public health importance, and many new control strategies target mating behaviour. Mating in this species occurs swarms characterised by male scramble competition female choice. These have a male-biased operational sex ratio, which is expected to generate intense among males for opportunities. However, it not known what proportion swarming successfully mate with females, how females each able with, extent any variation the success phenotype can...
Abstract Malaria parasites develop as oocysts in the mosquito for several days before they are able to infect a human host. During this time, mosquitoes take regular bloodmeals replenish their nutrient and energy reserves needed flight reproduction. We hypothesized that these critical oocyst growth experimental infection protocols, typically involving single bloodmeal at time of infection, cause nutritional stress developing oocysts. Therefore, enumerating disregarding differentiation state...
Abstract Malaria transmission requires Plasmodium parasites to successfully infect a female Anopheles mosquito, surviving series of robust innate immune responses. Understanding how evade these responses can highlight new ways block malaria transmission. We show that ookinete and sporozoite surface protein PIMMS43 is required for evasion the coluzzii complement-like system sporogonic development in oocyst. Disruption P. berghei triggers complement activation elimination upon mosquito midgut...