Halophilic archaea/bacteria adapt to different salt concentration, namely extreme, moderate and low. These type of adaptations may occur as a result modification protein structure other changes in cell organelles. Thus proteins play an important role the adaptation halophilic saline conditions. The Halophile database (HProtDB) is systematic attempt document biochemical biophysical properties from which be involved these organisms In this database, various physicochemical such molecular...

Since its emergence in Wuhan, China, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has spread very rapidly around the world, resulting a global pandemic. Though vaccination process started, number of COVID-affected patients is still quite large. Hence, an analysis hotspot mutations different evolving virus strains needs to be carried out. In this regard, multiple sequence alignment 71,038 SARS-CoV-2 genomes 98 countries over period from January 2020 June 2021 performed using...

β -D-Galactosidases (EC 3.2.1.23) hydrolyze the terminal nonreducing -D-galactose residues in -D-galactosides and are ubiquitously present all life forms including extremophiles. Eighteen microbial -galactosidase protein sequences, six each from psychrophilic, mesophilic, thermophilic microbes, were analyzed. Primary structure reveals alanine, glycine, serine, arginine to be higher psychrophilic -galactosidases whereas valine, glutamine, glutamic acid, phenylalanine, threonine, tyrosine...

In this paper, a modified method is used to approximate the solution time-fractional n-dimensional Navier–Stokes equation. The Variational Iteration Transform Method, which implemented in equation whose fractional order derivative described Caputo sense. proposed method's findings are presented and examined using figures. It demonstrated that efficient, dependable, simple apply various science engineering applications.

Abstract Whole genome analysis of SARS-CoV-2 is important to identify its genetic diversity. Moreover, accurate detection required for correct diagnosis. To address these, first we have analysed publicly available 10 664 complete or near-complete genomes 73 countries globally find mutation points in the coding regions as substitution, deletion, insertion and single nucleotide polymorphism (SNP) country wise. In this regard, multiple sequence alignment performed presence reference from NCBI....

Next-generation DNA sequencing (NGS) has made it feasible to sequence large number of microbial genomes and advancements in computational biology have opened enormous opportunities mine genome data for novel genes enzymes or their sources. In the present communication silico mining been carried out find sources nitrilases. The sequences selected were analyzed homology considered designing motifs. manually designed motifs based on amino acid nitrilases used screen 2000 (translated proteomes)....

In silico analysis of amino acid sequences some aromatic and aliphatic microbial nitrilases for certain physiochemical properties specificity to or nitriles has been done. The multiple sequence alignment shown clear differences between in terms position specific presence conserved acid. Statistical most the parameters did not show any distinction two nitrilases. residues besides active site domain triad (Glu, Lys, Cys) were His-129, Asn-168 Arg-174 these replaced by Arg-129, His -168 Lys-174...

A comparative study of amino acid sequence and physicochemical properties indicates the affiliation protein from nitrilase/cyanide hydratase family. This family contains nitrilases that break carbon-nitrogen bonds appear to be involved in reduction organic nitrogen compounds ammonia production. They all have distinct substrate specificity include nitrilase, cyanide hydratases, aliphatic amidases, beta-alanine synthase, a few other proteins with unknown molecular function. These sequences...

ABSTRACT Vaccination is a process that induces specific immune resistance to bacterial or viral infectious disease. Vaccines have prevented several diseases for many years, and are still being investigated. In late eighteenth century, Edward Jenner developed established the principle of vaccination using cross protection conferred by cowpox virus, which non pathogenic in humans. Regarding vaccine against periodontal disease, complexity periodontopathic bacteria might be problem determination...

Nitrilase is one of the nitrile metabolizing enzymes that catalyses conversion nitriles to corresponding acids which has gained importance in green chemistry. being substrate specific yet it acts over a wide range (aliphatic/aromatic) drawn attention due its utility mild hydrolysis. Most nitrilases reported hitherto have been physically extracted characterized from microbial/plant sources. In order identify sequences for two groups motif were designed i.e. aliphatic nitrilase motif’s (MDMAl)...

Proteases belong to the group of hydrolases which tend break chemical bond joining two amino acids together. Chymotrypsin first serine protease be discovered by scientists in our pancreas revolutionized their study both living system and applications industry. Computational tools techniques analyse identify proteases from organisms inhabiting extreme habitats has opened avenues as what contributes sequentially structurally whithstand pH or temperature. Keeping this view sixteen acid sequnces...